LOCUS       sequence-86868-e        1630 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: MT End Binding Protein, Excitation: 590, Emission: 
            649.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1630)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mPlum-EB1-8
FEATURES             Location/Qualifiers
     source          1..1630
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     62..78
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     62..78
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             908..1588
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     1309..1328
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1503..1526
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggactc agatctcgag ctccaccgcg gtggcggccg
       61 ctctagaact agtggatcca tggcagtgaa cgtatactca acgtcagtga ccagtgataa
      121 cctaagtcga catgacatgc tggcctggat caatgagtct ctgcagttga atctgacaaa
      181 gatcgaacag ttgtgctcag gggctgcgta ttgtcagttt atggacatgc tgttccctgg
      241 ctccattgcc ttgaagaaag tgaaattcca agctaagcta gaacacgagt acatccagaa
      301 cttcaaaata ttacaagcag gttttaagag aatgggtgtt gacaaaataa ttcctgtgga
      361 caaattagta aaaggaaagt ttcaggacaa ttttgaattc gttcagtggt tcaagaagtt
      421 cttcgatgca aactatgatg gaaaagacta tgaccctgtg gctgctagac aaggtcaaga
      481 aactgcagtg gctccttccc ttgttgctcc agctctgaat aaaccgaaga aacctctcac
      541 ttctagcagt gcagctcccc agaggcccat ctcaacacag agaaccgctg cggctcctaa
      601 ggctggccct ggtgtggtgc gaaagaaccc tggtgtgggc aacggagacg acgaggcagc
      661 tgagttgatg cagcaggtca acgtattgaa acttactgtt gaagacttgg agaaagagag
      721 ggatttctac ttcggaaagc tacggaacat tgaattgatt tgccaggaga acgaggggga
      781 aaacgaccct gtattgcaga ggattgtaga cattctgtat gccacagatg aaggctttgt
      841 gatacctgat gaagggggcc cacaggagga gcaagaagag tatacccggg atccaccggt
      901 ggctaccatg gtgagcaagg gcgaggaggt catcaaggag ttcatgcgct tcaaggagca
      961 catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
     1021 ctacgagggc acccagaccg ccaggctgaa ggtgaccaag ggtggccccc tgcccttcgc
     1081 ctgggacatc ctgtcccctc agatcatgta cggctccaag gcctacgtga agcaccccgc
     1141 cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
     1201 gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga
     1261 gttcatctac aaggtgaagg tgcgcggcac caacttcccc tccgacggcc ccgtaatgca
     1321 gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct
     1381 gaagggcgag atgaagatga ggctgaggct gaaggacggc ggccactacg acgccgaggt
     1441 caagaccacc tacatggcca agaagcccgt gcagctgccc ggcgcctaca agaccgacat
     1501 caagctggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgagcgcgc
     1561 cgagggccgc cactccaccg gcgcctaagc ggccgcgact ctagatcata atcagccata
     1621 ccacattgag
//
