LOCUS       sequence-86870-e        1445 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endosomes, Excitation: 590, Emission: 649.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1445)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 1445)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mPlum-Endo-14
FEATURES             Location/Qualifiers
     source          1..1445
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             33..710
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     434..453
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     628..651
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             756..785
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 cgtcagatcc gctagcgcta ccggtggcta ccatggtgag caagggcgag gaggtcatca
       61 aggagttcat gcgcttcaag gagcacatgg agggctccgt gaacggccac gagttcgaga
      121 tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccagg ctgaaggtga
      181 ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagatc atgtacggct
      241 ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg
      301 agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc
      361 aggactcctc cctgcaggac ggcgagttca tctacaaggt gaaggtgcgc ggcaccaact
      421 tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc
      481 ggatgtaccc cgaggacggc gccctgaagg gcgagatgaa gatgaggctg aggctgaagg
      541 acggcggcca ctacgacgcc gaggtcaaga ccacctacat ggccaagaag cccgtgcagc
      601 tgcccggcgc ctacaagacc gacatcaagc tggacatcac ctcccacaac gaggactaca
      661 ccatcgtgga acagtacgag cgcgccgagg gccgccactc caccggcgcc tccggatcag
      721 atctcgagct caagcttgat atcgaattcc ccatggagca gaagctgatc tccgaggagg
      781 acctgaaccg gaagaaactg gtggttgttg gtgatggagc ctgtggaaag acatgcttgc
      841 tcatagtctt cagcaaggac gagttcccag aggtgtatgt gcccacagtg tttgagaact
      901 atgtggcaga tatcgaggtg gatggaaagc aggtagagtt ggctttgtgg gacacagctg
      961 gccaggagga ctacgaccgc ctgcggccgc tctcctaccc ggacaccgac gtcattctca
     1021 tgtgcttctc ggtggacagc ccggactcgc tggagaacat ccccgagaag tgggtccccg
     1081 aggtgaagca cttctgtccc aatgtgccca tcatcctggt ggccaacaaa aaagacctgc
     1141 gcagcgacga gcatgtccgc acagagctgg cccgcatgaa gcaggaaccc gttcgcacgg
     1201 atgacggccg cgccatggcc gtgcgcatcc aagcctacga ctacctcgag tgctctgcca
     1261 agaccaagga aggcgtgcgc gaggtcttcg agacggccac gcgcgccgcg ctgcagaagc
     1321 gctacggctc ccagaacggc tgcatcaact gctgcaaggt gctatgaggg ccgcgcccgt
     1381 cgcgcctgcc cctgccgggg atccaccgga tctagataac tgatcataat cagccatacc
     1441 acatt
//
