LOCUS       sequence-86874-e        1746 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nuclear - Nucleolus, Excitation: 590, Emission: 649.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1746)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mPlum-Fibrillarin-7
FEATURES             Location/Qualifiers
     source          1..1746
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             35..712
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     436..455
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     630..653
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             complement(1508..1519)
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing 
                     et al., 2004)"
                     /label=TEE
                     /translation="NHKV"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtggc taccatggtg agcaagggcg aggaggtcat
       61 caaggagttc atgcgcttca aggagcacat ggagggctcc gtgaacggcc acgagttcga
      121 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca ggctgaaggt
      181 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcaga tcatgtacgg
      241 ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc
      301 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac
      361 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaaggtgc gcggcaccaa
      421 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga
      481 gcggatgtac cccgaggacg gcgccctgaa gggcgagatg aagatgaggc tgaggctgaa
      541 ggacggcggc cactacgacg ccgaggtcaa gaccacctac atggccaaga agcccgtgca
      601 gctgcccggc gcctacaaga ccgacatcaa gctggacatc acctcccaca acgaggacta
      661 caccatcgtg gaacagtacg agcgcgccga gggccgccac tccaccggcg cctccggact
      721 cagatctcga gccatgaagc caggattcag tccccgtggg ggtggctttg gcggccgagg
      781 gggctttggt gaccgtggtg gtcgtggagg ccgagggggc tttggcgggg gccgaggcca
      841 aggcggaggc tttagaggtc gtggacgagg aggaggtgga ggcggcggcg gcggtggagg
      901 aggaggaaga ggtggtggag gcttccattt tggtggcaac cggggtcgtg gtcggggagg
      961 aaaaagagga aaccagtcgg ggaagaatgt gatggtggag ccgcatcggc atgagggtgt
     1021 cttcatttgt cggggaaagg aagatgcact ggtcaccaag aacctggtcc ctggggaatc
     1081 agtttatgga gagaagagag tctcgatttc ggaaggagat gacaaaattg agtaccgagc
     1141 ctggaacccc ttccgctcca agctagcagc agcaatcctg ggtggtgtgg accagatcca
     1201 catcaaaccg ggggctaagg ttctctacct cggggctgcc tcgggcacca cggtctccca
     1261 tgtctctgac atcgttggtc cggatggtct agtctatgca gtcgagttct cccaccgctc
     1321 tggccgtgac ctcattaact tggccaagaa gaggaccaac atcattcctg tgatcgagga
     1381 tgctcgacac ccacacaaat accgcatgct catcgcaatg gtggatgtga tctttgctga
     1441 tgtggcccag ccagaccaga cccggattgt ggccctgaat gcccacacct tcctgcgtaa
     1501 tggaggacac tttgtgattt ccattaaggc caactgcatt gactccacag cctcagccga
     1561 ggccgtgttt gcctccgaag tgaaaaagat gcaacaggag aacatgaagc cgcaggagca
     1621 gttgaccctt gagccatatg aaagagacca tgccgtggtc gtgggagtgt acaggccacc
     1681 ccccaaggtg aagaactgag gatccaccgg atctagataa ctgatcataa tcagccatac
     1741 cacatt
//
