LOCUS       sequence-86877-e        1140 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Golgi, Excitation: 590, Emission: 649.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1140)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 1140)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mPlum-Golgi-3
FEATURES             Location/Qualifiers
     source          1..1140
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             340..1020
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     741..760
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     935..958
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 tccgccccat gacgcaaatg gcgtagcgtg tacgtggagg tctatataag cagagctgtt
       61 tagtgaaccg tcagatccgc tagcatgagg cttcgggagc cgctcctgag cggcagcgcc
      121 gcgatgccag gcgcgtccct acagcgggcc tgccgcctgc tcgtggccgt ctgcgctctg
      181 caccttggcg tcaccctcgt ttactacctg gctggccgcg acctgagccg cctgccccaa
      241 ctggtcggag tctccacacc gctgcagggc ggctcgaaca gtgccgccgc catcgggcag
      301 tcctccgggg agctccggac cggaggggcc aaggatccaa tggtgagcaa gggcgaggag
      361 gtcatcaagg agttcatgcg cttcaaggag cacatggagg gctccgtgaa cggccacgag
      421 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaggctg
      481 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagatcatg
      541 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc
      601 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc
      661 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa ggtgcgcggc
      721 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
      781 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatgaagat gaggctgagg
      841 ctgaaggacg gcggccacta cgacgccgag gtcaagacca cctacatggc caagaagccc
      901 gtgcagctgc ccggcgccta caagaccgac atcaagctgg acatcacctc ccacaacgag
      961 gactacacca tcgtggaaca gtacgagcgc gccgagggcc gccactccac cggcgcctaa
     1021 gcggccgcga ctctagatca taatcagcca taccacattt gtagaggttt tacttgcttt
     1081 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
//
