LOCUS       sequence-86880-e        1174 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 590, Emission: 649.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1174)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mPlum-H2B-2
FEATURES             Location/Qualifiers
     source          1..1174
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             71..448
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             455..1135
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     856..875
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1050..1073
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 atccgctagc gctaccggac tcagatctcg agctcaagct tcgaattctg cagtcgacgg
       61 taccgccacc atgccagagc cagcgaagtc tgctcccgcc ccgaaaaagg gctccaagaa
      121 ggcggtgact aaggcgcaga agaaaggcgg caagaagcgc aagcgcagcc gcaaggagag
      181 ctattccatc tatgtgtaca aggttctgaa gcaggtccac cctgacaccg gcatttcgtc
      241 caaggccatg ggcatcatga attcgtttgt gaacgacatt ttcgagcgca tcgcaggtga
      301 ggcttcccgc ctggcgcatt acaacaagcg ctcgaccatc acctccaggg agatccagac
      361 ggccgtgcgc ctgctgctgc ctggggagtt ggccaagcac gccgtgtccg agggtactaa
      421 ggccatcacc aagtacacca gcgctaagga tccaatggtg agcaagggcg aggaggtcat
      481 caaggagttc atgcgcttca aggagcacat ggagggctcc gtgaacggcc acgagttcga
      541 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca ggctgaaggt
      601 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcaga tcatgtacgg
      661 ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc
      721 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac
      781 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaaggtgc gcggcaccaa
      841 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga
      901 gcggatgtac cccgaggacg gcgccctgaa gggcgagatg aagatgaggc tgaggctgaa
      961 ggacggcggc cactacgacg ccgaggtcaa gaccacctac atggccaaga agcccgtgca
     1021 gctgcccggc gcctacaaga ccgacatcaa gctggacatc acctcccaca acgaggacta
     1081 caccatcgtg gaacagtacg agcgcgccga gggccgccac tccaccggcg cctaagcggc
     1141 cgcgactcta gatcataatc agccatacca catt
//
