LOCUS       sequence-86881-e        1196 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 590, Emission: 649.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Olenych SG, Claxton NS, Ottenberg GK, Davidson MW
  TITLE     The fluorescent protein color palette.
  JOURNAL   Curr Protoc Cell Biol. 2007 Sep;Chapter 21:Unit 21.5. doi: 
            10.1002/0471143030.cb2105s36.
  PUBMED    18228502
REFERENCE   2  (bases 1 to 1196)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Protoc
            Cell Biol."; date: "2007-09"; volume: "Chapter 21:Unit 21.5. doi"; 
            pages: " 10.1002/0471143030.cb2105s36"
COMMENT     Sequence Label: mPlum-H2B-6
FEATURES             Location/Qualifiers
     source          1..1196
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             78..455
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             474..1154
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     875..894
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1069..1092
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggactca gatctcgagc tcaagcttcg aattctgcag
       61 tcgacggtac cgccaccatg ccagagccag cgaagtctgc tcccgccccg aaaaagggct
      121 ccaagaaggc ggtgactaag gcgcagaaga aaggcggcaa gaagcgcaag cgcagccgca
      181 aggagagcta ttccatctat gtgtacaagg ttctgaagca ggtccaccct gacaccggca
      241 tttcgtccaa ggccatgggc atcatgaatt cgtttgtgaa cgacattttc gagcgcatcg
      301 caggtgaggc ttcccgcctg gcgcattaca acaagcgctc gaccatcacc tccagggaga
      361 tccagacggc cgtgcgcctg ctgctgcctg gggagttggc caagcacgcc gtgtccgagg
      421 gtactaaggc catcaccaag tacaccagcg ctaaggatcc accggtggct accatggtga
      481 gcaagggcga ggaggtcatc aaggagttca tgcgcttcaa ggagcacatg gagggctccg
      541 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc
      601 agaccgccag gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt
      661 cccctcagat catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact
      721 acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg
      781 gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg
      841 tgaaggtgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg
      901 gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatga
      961 agatgaggct gaggctgaag gacggcggcc actacgacgc cgaggtcaag accacctaca
     1021 tggccaagaa gcccgtgcag ctgcccggcg cctacaagac cgacatcaag ctggacatca
     1081 cctcccacaa cgaggactac accatcgtgg aacagtacga gcgcgccgag ggccgccact
     1141 ccaccggcgc ctaagcggcc gcgactctag atcataatca gccataccac attgag
//
