LOCUS       sequence-86883-e        1221 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 488, Emission: 507.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1221)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 1221)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mPlum-H3-23
FEATURES             Location/Qualifiers
     source          1..1221
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    436..488
                     /label=MCS
                     /note="multiple cloning site"
     CDS             502..1182
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     903..922
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1097..1120
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 cgtcaagatc cgctagccgc caccatggct cgtactaaac agacagctcg gaaatccacc
       61 ggcggtaaag cgccacgcaa gcagctggct accaaggctg ctcgcaagag cgcgccggct
      121 accggcggcg tgaaaaagcc tcaccgttac cgcccgggca ctgtggctct gcgcgagatc
      181 cgccgctacc aaaagtcgac cgagttgctg attcggaagc tgccgttcca gcgcctggtg
      241 cgagaaatcg cccaagactt caagaccgat cttcgcttcc agagctctgc ggtgatggcg
      301 ctgcaggagg cttgtgaggc ctacttggta gggctctttg aggacacaaa cctttgcgcc
      361 atccatgcta agcgagtgac tattatgccc aaagacatcc agctcgctcg ccgcattcgc
      421 ggagaaagag cggatctcga gctcaagctt cgaattctgc agtcgacggt accgcgggcc
      481 cgggatccac cggtggctac catggtgagc aagggcgagg aggtcatcaa ggagttcatg
      541 cgcttcaagg agcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
      601 ggcgagggcc gcccctacga gggcacccag accgccaggc tgaaggtgac caagggtggc
      661 cccctgccct tcgcctggga catcctgtcc cctcagatca tgtacggctc caaggcctac
      721 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag
      781 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
      841 ctgcaggacg gcgagttcat ctacaaggtg aaggtgcgcg gcaccaactt cccctccgac
      901 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc
      961 gaggacggcg ccctgaaggg cgagatgaag atgaggctga ggctgaagga cggcggccac
     1021 tacgacgccg aggtcaagac cacctacatg gccaagaagc ccgtgcagct gcccggcgcc
     1081 tacaagaccg acatcaagct ggacatcacc tcccacaacg aggactacac catcgtggaa
     1141 cagtacgagc gcgccgaggg ccgccactcc accggcgcct aagcggccgc gactctagat
     1201 cataatcagc cataccacat t
//
