LOCUS       sequence-86884-e        1124 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 488, Emission: 507.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1124)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 1124)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mPlum-H4-23
FEATURES             Location/Qualifiers
     source          1..1124
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    339..391
                     /label=MCS
                     /note="multiple cloning site"
     CDS             405..1085
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     806..825
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1000..1023
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 gaccgtcaga tccgctagcc gccaccatgt ctggccgcgg caaaggcggg aagggtcttg
       61 gcaaaggcgg cgctaagcgc caccgtaaag tactgcgcga caatatccag ggcatcacca
      121 agccggccat ccggcgcctt gctcgccgcg gcggcgtgaa gcgcatctcc ggcctcatct
      181 acgaggagac tcgcggggtg ctgaaggtgt tcctggagaa cgtgatccgg gacgccgtga
      241 cctatacaga gcacgccaag cgcaagacgg tcaccgccat ggatgtggtc tacgcgctca
      301 agcgccaggg ccgcaccctc tacggtttcg gtggggatct cgagctcaag cttcgaattc
      361 tgcagtcgac ggtaccgcgg gcccgggatc caccggtggc taccatggtg agcaagggcg
      421 aggaggtcat caaggagttc atgcgcttca aggagcacat ggagggctcc gtgaacggcc
      481 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca
      541 ggctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcaga
      601 tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc
      661 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg
      721 tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaaggtgc
      781 gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg
      841 cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatg aagatgaggc
      901 tgaggctgaa ggacggcggc cactacgacg ccgaggtcaa gaccacctac atggccaaga
      961 agcccgtgca gctgcccggc gcctacaaga ccgacatcaa gctggacatc acctcccaca
     1021 acgaggacta caccatcgtg gaacagtacg agcgcgccga gggccgccac tccaccggcg
     1081 cctaagcggc cgcgactcta gatcataatc agccatacca catt
//
