LOCUS       sequence-86885-e        2083 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Note- This plasmid contains Keratin 18. Localization: Cytokeratin, 
            Excitation: 590, Emission: 649.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2083)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mPlum-Keratin-17
FEATURES             Location/Qualifiers
     source          1..2083
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1364..2044
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     1765..1784
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1959..1982
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 gaacgtcaga tccgctagca ccatgagctt caccactcgc tccaccttct ccaccaacta
       61 ccggtccctg ggctctgtcc aggcgcccag ctacggcgcc cggccggtca gcagcgcggc
      121 cagcgtctat gcaggcgctg ggggctctgg ttcccggatc tccgtgtccc gctccaccag
      181 cttcaggggc ggcatggggt ccgggggcct ggccaccggg atagccgggg gtctggcagg
      241 aatgggaggc atccagaacg agaaggagac catgcaaagc ctgaacgacc gcctggcctc
      301 ttacctggac agagtgagga gcctggagac cgagaaccgg aggctggaga gcaaaatccg
      361 ggagcacttg gagaagaagg gaccccaggt cagagactgg agccattact tcaagatcat
      421 cgaggacctg agggctcaga tcttcgcaaa tactgtggac aatgcccgca tcgttctgca
      481 gattgacaat gcccgtcttg ctgctgatga ctttagagtc aagtatgaga cagagctggc
      541 catgcgccag tctgtggaga acgacatcca tgggctccgc aaggtcattg atgacaccaa
      601 tatcacacga ctgcagctgg agacagagat cgaggctctc aaggaggagc tgctcttcat
      661 gaagaagaac cacgaagagg aagtaaaagg cctacaagcc cagattgcca gctctgggtt
      721 gaccgtggag gtagatgccc ccaaatctca ggacctcgcc aagatcatgg cagacatccg
      781 ggcccaatat gacgagctgg ctcggaagaa ccgagaggag ctagacaagt actggtctca
      841 gcagattgag gagagcacca cagtgctcac cacacagtct gctgaggttg gagctgctga
      901 gacgacgctc acagagctga gacgtacagt ccagtccttg gagatcgacc tggactccat
      961 gagaaatctg aaggccagct tggagaacag cctgagggag gtggaggccc gctacgccct
     1021 acagatggag cagctcaacg ggatcctgct gcaccttgag tcagagctgg cacagacccg
     1081 ggcagaggga cagcgccagg cccaggagta tgaggccctg ctgaacatca aggtcaagct
     1141 ggaggctgag atcgccacct accgccgcct gctggaagat ggcgaggact ttaatcttgg
     1201 tgatgccttg gacagcagca actccatgca aaccatccaa aagaccacca cccgccggat
     1261 agtggatggc aaagtggtgt ctgagaccaa tgacaccaaa gttctgaggc atcgaattct
     1321 gcagtcgacg gtaccgcggg cccgggatcc accggtggct accatggtga gcaagggcga
     1381 ggaggtcatc aaggagttca tgcgcttcaa ggagcacatg gagggctccg tgaacggcca
     1441 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccag
     1501 gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagat
     1561 catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct
     1621 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt
     1681 gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg tgaaggtgcg
     1741 cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc
     1801 ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatga agatgaggct
     1861 gaggctgaag gacggcggcc actacgacgc cgaggtcaag accacctaca tggccaagaa
     1921 gcccgtgcag ctgcccggcg cctacaagac cgacatcaag ctggacatca cctcccacaa
     1981 cgaggactac accatcgtgg aacagtacga gcgcgccgag ggccgccact ccaccggcgc
     2041 ctaagcggcc gcgactctag atcataatca gccataccac att
//
