LOCUS       sequence-86886-e        2526 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nuclear Envelope, Excitation: 590, Emission: 649.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2526)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mPlum-LaminB1-10
FEATURES             Location/Qualifiers
     source          1..2526
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             35..712
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     436..455
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     630..653
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtggc taccatggtg agcaagggcg aggaggtcat
       61 caaggagttc atgcgcttca aggagcacat ggagggctcc gtgaacggcc acgagttcga
      121 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca ggctgaaggt
      181 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcaga tcatgtacgg
      241 ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc
      301 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac
      361 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaaggtgc gcggcaccaa
      421 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga
      481 gcggatgtac cccgaggacg gcgccctgaa gggcgagatg aagatgaggc tgaggctgaa
      541 ggacggcggc cactacgacg ccgaggtcaa gaccacctac atggccaaga agcccgtgca
      601 gctgcccggc gcctacaaga ccgacatcaa gctggacatc acctcccaca acgaggacta
      661 caccatcgtg gaacagtacg agcgcgccga gggccgccac tccaccggcg cctccggact
      721 cagatctcga gctcaagctt cgatggcgac tgcgaccccc gtgccgccgc ggatgggcag
      781 ccgcgctggc ggccccacca cgccgctgag ccccacgcgc ctgtcgcggc tccaggagaa
      841 ggaggagctg cgcgagctca atgaccggct ggcggtgtac atcgacaagg tgcgcagcct
      901 ggagacggag aacagcgcgc tgcagctgca ggtgacggag cgcgaggagg tgcgcggccg
      961 tgagctcacc ggcctcaagg cgctctacga gaccgagctg gccgacgcgc gacgcgcgct
     1021 cgacgacacg gcccgcgagc gcgccaagct gcagatcgag ctgggcaagt gcaaggcgga
     1081 acacgaccag ctgctcctca actatgctaa gaaggaatct gatcttaatg gcgcccagat
     1141 caagcttcga gaatatgaag cagcactcaa ttcgaaagat gcagctcttg ctactgcact
     1201 tggtgacaaa aaaagtttag agggagattt ggaggatctg aaggatcaga ttgcccagtt
     1261 ggaagcctcc ttagctgcag ccaaaaaaca gttagcagat gaaactttac ttaaagtaga
     1321 tttggagaat cgttgtcaga gccttactga ggacttggag tttcgcaaaa gcatgtatga
     1381 agaggagatt aacgagacca gaaggaagca tgaaacgcgc ttggtagagg tggattctgg
     1441 gcgtcaaatt gagtatgagt acaagctggc gcaagccctt catgagatga gagagcaaca
     1501 tgatgcccaa gtgaggctgt ataaggagga gctggagcag acttaccatg ccaaacttga
     1561 gaatgccaga ctgtcatcag agatgaatac ttctactgtc aacagtgcca gggaagaact
     1621 gatggaaagc cgcatgagaa ttgagagcct ttcatcccag ctttctaatc tacagaaaga
     1681 gtctagagca tgtttggaaa ggattcaaga attagaggac ttgcttgcta aagaaaaaga
     1741 caactctcgt cgcatgctga cagacaaaga gagagagatg gcggaaataa gggatcaaat
     1801 gcagcaacag ctgaatgact atgaacagct tcttgatgta aagttagccc tggacatgga
     1861 aatcagtgct tacaggaaac tcttagaagg cgaagaagag aggttgaagc tgtctccaag
     1921 cccttcttcc cgtgtgacag tatcccgagc atcctcaagt cgtagtgtac gtacaactag
     1981 aggaaagcgg aagagggttg atgtggaaga atcagaggcg agtagtagtg ttagcatctc
     2041 tcattccgcc tcagccactg gaaatgtttg catcgaagaa attgatgttg atgggaaatt
     2101 tatccgcttg aagaacactt ctgaacagga tcaaccaatg ggaggctggg agatgatcag
     2161 aaaaattgga gacacatcag tcagttataa atatacctca agatatgtgc tgaaggcagg
     2221 ccagactgtt acaatttggg ctgcaaacgc tggtgtcaca gccagccccc caactgacct
     2281 catctggaag aaccagaact cgtggggcac tggcgaagat gtgaaggtta tattgaaaaa
     2341 ttctcaggga gaggaggttg ctcaaagaag tacagtcttt aaaacaacca tacctgaaga
     2401 agaggaggag gaggaagaag cagctggagt ggttgttgag gaagaacttt tccaccagca
     2461 gggaacccca agagcatcca atagaagctg tgcaattatg tagggatcca ccggatctag
     2521 ataact
//
