LOCUS       sequence-86887-e        2038 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Lysosome Membrane, Excitation: 590, Emission: 649.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2038)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mPlum-Lysosomes-20
FEATURES             Location/Qualifiers
     source          1..2038
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      39..48
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      43..52
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(45..107,108..1265)
                     /codon_start=1
                     /product="rat lysosome-associated membrane glycoprotein 1"
                     /label=LAMP1
                     /translation="MAAPGARRPLLLLLLAGLAHSAPALFEVKDNNGTACIMASFSASF
                     LTTYEAGHVSKVSNMTLPASAEVLKNSSSCGEKNASEPTLAITFGEGYLLKLTFTKNTT
                     RYSVQHMYFTYNLSDTQFFPNASSKGPDTVDSTTDIKADINKTYRCVSDIRVYMKNVTI
                     VLWDATIQAYLPSSNFSKEETRCPQDQPSPTTGPPSPSPPLVPTNPSVSKYNVTGDNGT
                     CLLASMALQLNITYMKKDNTTVTRAFNINPSDKYSGTCGAQLVTLKVGNKSRVLELQFG
                     MNATSSLFFLQGVQLNMTLPDAIEPTFSTSNYSLKALQASVGNSYKCNSEEHIFVSKAL
                     ALNVFSVQVQAFRVESDRFGSVEECVQDGNNMLIPIAVGGALAGLVLIVLIAYLIGRKR
                     SHAGYQTI"
     CDS             1326..2006
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     1727..1746
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1921..1944
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 gtcagatccg ctagcgctac cggactcaga tctcgagcgc caccatggcg gccccgggcg
       61 cccggcggcc gctgctcctg ttgctgctgg caggccttgc acacagcgcc ccagcactgt
      121 tcgaggtgaa agacaacaac ggcacagcgt gtataatggc cagcttctct gcctcctttc
      181 tgaccaccta tgaggctgga catgtttcta aggtctcgaa tatgaccctg ccagcctctg
      241 cagaagtcct gaagaatagc agctcttgtg gtgaaaagaa tgcttctgag cccaccctcg
      301 caatcacctt tggagaagga tatttactga aactcacctt cacaaaaaac acaacacgtt
      361 acagtgtcca gcacatgtat ttcacatata acctgtcaga cacacaattc tttcccaatg
      421 ccagctccaa agggcccgac actgtggatt ccacaactga catcaaggca gacatcaaca
      481 aaacataccg atgtgtcagc gacatcaggg tctacatgaa gaatgtgacc attgtgctct
      541 gggacgctac tatccaggcc tacctgccga gtagcaactt cagcaaggaa gagacacgct
      601 gcccacagga tcaaccttcc ccaactactg ggccacccag cccctcacca ccacttgtgc
      661 ccacaaaccc cagtgtgtcc aagtacaatg tgactggtga caatggaacc tgcctgctgg
      721 cctctatggc actgcaactc aacatcacct acatgaagaa ggacaacacg actgtgacca
      781 gagcattcaa catcaaccca agtgacaaat atagtgggac ttgcggtgcc cagttggtga
      841 ccctgaaggt ggggaacaag agcagagtcc tggagctgca gtttgggatg aatgccactt
      901 ctagcctgtt tttcctgcaa ggagttcagt tgaacatgac tcttcctgat gccatagagc
      961 ccacgttcag cacctccaac tattccctga aagctcttca ggccagtgtc ggcaactcat
     1021 acaagtgcaa cagtgaggag cacatctttg tcagcaaggc gctcgccctc aatgtcttca
     1081 gcgtgcaagt ccaggctttc agggtagaaa gtgacaggtt tgggtctgtg gaagagtgtg
     1141 tacaggacgg taacaacatg ctgatcccca ttgctgtggg cggggccctg gcagggctgg
     1201 tcctcatcgt cctcatcgcc tacctcatcg gcaggaagag gagtcacgcg ggctatcaga
     1261 ccatctcgga attcggctcc accggctcca ccggctccac cggcgcggat ccaccggtgg
     1321 ctaccatggt gagcaagggc gaggaggtca tcaaggagtt catgcgcttc aaggagcaca
     1381 tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct
     1441 acgagggcac ccagaccgcc aggctgaagg tgaccaaggg tggccccctg cccttcgcct
     1501 gggacatcct gtcccctcag atcatgtacg gctccaaggc ctacgtgaag caccccgccg
     1561 acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga
     1621 acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt
     1681 tcatctacaa ggtgaaggtg cgcggcacca acttcccctc cgacggcccc gtaatgcaga
     1741 agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga
     1801 agggcgagat gaagatgagg ctgaggctga aggacggcgg ccactacgac gccgaggtca
     1861 agaccaccta catggccaag aagcccgtgc agctgcccgg cgcctacaag accgacatca
     1921 agctggacat cacctcccac aacgaggact acaccatcgt ggaacagtac gagcgcgccg
     1981 agggccgcca ctccaccggc gcctaagcgg ccgcgactct agatcataat cagccata
//
