LOCUS       sequence-86889-e        1104 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mannosidase/Golgi, Excitation: 590, Emission: 649.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1104)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 1104)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mPlum-MannII-N-10
FEATURES             Location/Qualifiers
     source          1..1104
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             385..1065
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     786..805
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     980..1003
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 agatccgcta gcgccaccat gaagttaagt cgccagttca ccgtgtttgg cagcgcgatc
       61 ttctgcgtcg taatcttctc actctacctg atgctggaca ggggtcactt ggactaccct
      121 cggggcccgc gccaggaggg ctcctttccg cagggccagc tttcaatatt gcaagaaaag
      181 attgaccatt tggagcgttt gctcgctgag aacaacgaga tcatctcaaa tatcagagac
      241 tcagtcatca acctgagcga gtctgtggag gacggcccgc gggggtcacc aggcaacgcc
      301 agccaaggct ccatccacct ccactcgcca cagttggccc tgcaggctga ccccagctcc
      361 ggatcggatc caccggtggc taccatggtg agcaagggcg aggaggtcat caaggagttc
      421 atgcgcttca aggagcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
      481 gagggcgagg gccgccccta cgagggcacc cagaccgcca ggctgaaggt gaccaagggt
      541 ggccccctgc ccttcgcctg ggacatcctg tcccctcaga tcatgtacgg ctccaaggcc
      601 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
      661 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
      721 tccctgcagg acggcgagtt catctacaag gtgaaggtgc gcggcaccaa cttcccctcc
      781 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
      841 cccgaggacg gcgccctgaa gggcgagatg aagatgaggc tgaggctgaa ggacggcggc
      901 cactacgacg ccgaggtcaa gaccacctac atggccaaga agcccgtgca gctgcccggc
      961 gcctacaaga ccgacatcaa gctggacatc acctcccaca acgaggacta caccatcgtg
     1021 gaacagtacg agcgcgccga gggccgccac tccaccggcg cctaagcggc cgcgactcta
     1081 gatcataatc agccatacca catt
//
