LOCUS       sequence-86891-e        1842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: 590, Emission: 649.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1842)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mPlum-MAPTau-N-10
FEATURES             Location/Qualifiers
     source          1..1842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      34..43
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1120..1129
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1126..1806
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     1527..1546
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1721..1744
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 aatccgctag cgctaccgga ctcagatctc gaggccacca tggctgagcc ccgccaggag
       61 ttcgaagtga tggaagatca cgctgggacg tacgggttgg gggacaggaa agatcagggg
      121 ggctacacca tgcaccaaga ccaagagggt gacacggacg ctggcctgaa agctgaagaa
      181 gcaggcattg gagacacccc cagcctggaa gacgaagctg ctggtcacgt gacccaagct
      241 cgcatggtca gtaaaagcaa agacgggact ggaagcgatg acaaaaaagc caagggggct
      301 gatggtaaaa cgaagatcgc cacaccgcgg ggagcagccc ctccaggcca gaagggccag
      361 gccaacgcca ccaggattcc agcaaaaacc ccgcccgctc caaagacacc acccagctct
      421 ggtgaacctc caaaatcagg ggatcgcagc ggctacagca gccccggctc cccaggcact
      481 cccggcagcc gctcccgcac cccgtccctt ccaaccccac ccacccggga gcccaagaag
      541 gtggcagtgg tccgtactcc acccaagtcg ccgtcttccg ccaagagccg cctgcagaca
      601 gcccccgtgc ccatgccaga cctgaagaat gtcaagtcca agatcggctc cactgagaac
      661 ctgaagcacc agccgggagg cgggaaggtg caaatagtct acaaaccagt tgacctgagc
      721 aaggtgacct ccaagtgtgg ctcattaggc aacatccatc ataaaccagg aggtggccag
      781 gtggaagtaa aatctgagaa gcttgacttc aaggacagag tccagtcgaa gattgggtcc
      841 ctggacaata tcacccacgt ccctggcgga ggaaataaaa agattgaaac ccacaagctg
      901 accttccgcg agaacgccaa agccaagaca gaccacgggg cggagatcgt gtacaagtcg
      961 ccagtggtgt ctggggacac gtctccacgg catctcagca atgtctcctc caccggcagc
     1021 atcgacatgg tagactcgcc ccagctcgcc acgctagctg acgaggtgtc tgcctccctg
     1081 gccaagcagg gtttgagctc cggaggggat ccaccggtcg ccaccatggt gagcaagggc
     1141 gaggaggtca tcaaggagtt catgcgcttc aaggagcaca tggagggctc cgtgaacggc
     1201 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
     1261 aggctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
     1321 atcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag
     1381 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
     1441 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaaggtg
     1501 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
     1561 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat gaagatgagg
     1621 ctgaggctga aggacggcgg ccactacgac gccgaggtca agaccaccta catggccaag
     1681 aagcccgtgc agctgcccgg cgcctacaag accgacatca agctggacat cacctcccac
     1741 aacgaggact acaccatcgt ggaacagtac gagcgcgccg agggccgcca ctccaccggc
     1801 gcctaagcgg ccgcgactct agatcataat cagccatacc aa
//
