LOCUS       sequence-86893-e         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mitochondria, Excitation: 590, Emission: 649.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Day RN, Davidson MW
  TITLE     The fluorescent protein palette: tools for cellular imaging.
  JOURNAL   Chem Soc Rev. 2009 Oct;38(10):2887-921. doi: 10.1039/b901966a. Epub 
            2009 Aug 4.
  PUBMED    19771335
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1039/b901966a"; 
            journalName: "Chem Soc Rev"; date: "2009-10"; volume: "38"; issue: 
            "10"; pages: "2887-921"
COMMENT     Sequence Label: mPlum-Mito-3
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             17..91
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             113..793
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     514..533
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     708..731
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 cgtcagatcc gctagcatgt ccgtcctgac gccgctgctg ctgcggggct tgacaggctc
       61 ggcccggcgg ctcccagtgc cgcgcgccaa gatccattcg ttgggggatc caatggtgag
      121 caagggcgag gaggtcatca aggagttcat gcgcttcaag gagcacatgg agggctccgt
      181 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
      241 gaccgccagg ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc
      301 ccctcagatc atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta
      361 cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
      421 cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt
      481 gaaggtgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg
      541 ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatgaa
      601 gatgaggctg aggctgaagg acggcggcca ctacgacgcc gaggtcaaga ccacctacat
      661 ggccaagaag cccgtgcagc tgcccggcgc ctacaagacc gacatcaagc tggacatcac
      721 ctcccacaac gaggactaca ccatcgtgga acagtacgag cgcgccgagg gccgccactc
      781 caccggcgcc taagcggccg cgactctaga tcataatcag ccataccaca tttgtag
//
