LOCUS       sequence-86894-e        6724 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 590, Emission: 649.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6724)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 6724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mPlum-MyosinIIA-C-18
FEATURES             Location/Qualifiers
     source          1..6724
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      32..41
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             38..715
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     439..458
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     633..656
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 gtgaccgctc gagccgctag cgctaccggt cgccaccatg gtgagcaagg gcgaggaggt
       61 catcaaggag ttcatgcgct tcaaggagca catggagggc tccgtgaacg gccacgagtt
      121 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaggctgaa
      181 ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agatcatgta
      241 cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt
      301 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt
      361 gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagg tgcgcggcac
      421 caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc
      481 cgagcggatg taccccgagg acggcgccct gaagggcgag atgaagatga ggctgaggct
      541 gaaggacggc ggccactacg acgccgaggt caagaccacc tacatggcca agaagcccgt
      601 gcagctgccc ggcgcctaca agaccgacat caagctggac atcacctccc acaacgagga
      661 ctacaccatc gtggaacagt acgagcgcgc cgagggccgc cactccaccg gcgcctccgg
      721 actcagatct ggcagcggtg gaggcagcgc atccggcgga agcggaagcg ctcagcaggc
      781 tgcagacaag tacctctatg tggataaaaa cttcatcaat aacccgctgg cccaagctga
      841 ctgggctgcc aagaagttgg tatgggtgcc ttccagcaag aatggctttg aaccagccag
      901 cctcaaggag gaggtgggag aagaggccat tgtagagctg gtagagaatg ggaagaaggt
      961 gaaggtgaac aaggacgaca tccagaagat gaacccaccc aagttctcca aggtggagga
     1021 catggcagag ctcacgtgcc tcaacgaagc ttcggtgctg cacaacctca aggagcgata
     1081 ctactcaggg cttatctaca cctattcagg cctgttctgt gtggtcatca acccttataa
     1141 gaacctgccc atctactcag aggagatcgt ggagatgtac aagggcaaga agaggcacga
     1201 gatgccaccc cacatctacg ccatcacaga tactgcctac cggagcatga tgcaggaccg
     1261 ggaagatcag tccatcctgt gcacggggga gtctggagca gggaagacag agaacaccaa
     1321 gaaagtcatc cagtacctgg cacatgtggc ctcctcacac aagagcaaga aggaccaggg
     1381 ggagttggag cggcagctgc tacaggccaa ccctatccta gaggcctttg gaaacgccaa
     1441 gacggtgaag aatgacaact cctctcgatt cggtaaattc attcgtatca actttgatgt
     1501 caatggctac attgttggtg ccaacattga gacttatctt ctggagaaat ctcgtgctat
     1561 ccgccaagcc aaagaggagc ggaccttcca catcttctac tacctgctgt ctggggccgg
     1621 agaacacctg aagactgatc tcctgttgga gccatacaac aaataccgct tcctgtccaa
     1681 cgggcacgtc accatccctg ggcagcagga caaggacatg ttccaggaga caatggaggc
     1741 catgagaatt atgggtatcc cagaggatga gcagatgggc ttgctgcggg tcatctctgg
     1801 ggtccttcag cttggcaaca ttgccttcaa gaaggagcgg aacactgacc aggcgtccat
     1861 gccggacaac acagctgctc aaaaggtgtc ccacctcctg gggatcaatg tgaccgactt
     1921 caccagaggc atcctcaccc cacgcatcaa ggtgggcaga gactatgtgc agaaggcgca
     1981 gactaaagag caggctgact ttgccattga ggccttggcc aaggctacct atgagcggat
     2041 gttccgctgg ctggtgcttc gcatcaacaa agctctggac aagaccaaga ggcagggcgc
     2101 ctcatttatc gggatactgg acatcgctgg ctttgaaatc tttgatctga actccttcga
     2161 gcagctgtgc atcaactaca ccaacgagaa gctgcagcag ctgttcaacc acaccatgtt
     2221 catcctggag caggaggagt accagcgaga gggcatcgag tggaacttca tcgacttcgg
     2281 cctggacctg cagccctgca tcgacctcat tgagaagccg gcgggtcccc caggcatcct
     2341 ggccctgcta gatgaggagt gctggtttcc taaggccact gacaagagct tcgtggagaa
     2401 ggtggtgcag gagcagggca cccaccccaa gttccagaag cccaagcaac tgaaggacaa
     2461 ggctgatttc tgcattatcc actatgccgg caaggtggac tataaagctg acgagtggct
     2521 gatgaagaac atggacccct tgaacgacaa catcgccacg ctgcttcacc agtcctcaga
     2581 caagtttgtc tctgagctgt ggaaggatgt ggatcggatc attggcttgg accaagtggc
     2641 tggaatgtcc gagacagcac tacctggtgc cttcaagacc cggaagggca tgttccgtac
     2701 tgtcggacag ctgtacaagg agcagctggc caagctcatg gccacgttga ggaataccaa
     2761 ccccaacttc gtgcgctgca tcattcccaa ccatgagaag aaggccggca aactggaccc
     2821 gcacttggtg ctggaccagc tgcgctgcaa tggcgtcctt gagggcatcc ggatctgccg
     2881 ccagggcttt cccaacaggg tggtcttcca ggagttccgg cagaggtatg agatcctcac
     2941 ccccaactcc atcccgaagg gcttcatgga tggcaagcaa gcgtgtgtgc tcatgatcaa
     3001 agccttggag cttgacagca acctgtaccg catcggccag agcaaagtgt tcttccgggc
     3061 aggagtgctc gcccacctgg aggaagagcg ggacctgaag atcaccgatg tcatcattgg
     3121 cttccaggcc tgctgcaggg gctacctggc caggaaggcc tttgccaaga ggcagcaaca
     3181 gctgaccgcc atgaaggtcc tacagaggaa ctgtgctgcg tacctcaggc tgcgcaactg
     3241 gcagtggtgg aggctcttca ccaaggtcaa gcccctgttg aactcaataa gacatgagga
     3301 tgagctgtta gccaaggagg cggaactgac aaaggttcga gagaaacatc tggctgcaga
     3361 gaacaggctg acagagatgg agacgatgca gtctcagctc atggcagaga agctgcagct
     3421 tcaggagcag ctgcaggcgg agacagagct gtgtgccgag gctgaggagc tccgggcccg
     3481 tctgacagcg aagaagcagg agctggagga gatatgccat gacctggagg ccagggtgga
     3541 ggaggaggag gagcgctgcc agtacctgca ggccgagaag aagaagatgc agcagaacat
     3601 ccaggaactt gaggagcagt tggaggagga ggagagcgcc cggcagaagc tgcagcttga
     3661 gaaggtgacc accgaggcca agctgaagaa actggaggag gaccagatca tcatggagga
     3721 ccagaactgc aaactggcca aggagaagaa actgctggaa gacagagtag ctgagttcac
     3781 taccaacctc atggaagagg aggagaagtc caagagcctg gccaagctca agaacaagca
     3841 cgaggcaatg atcaccgacc tggaagagcg cctccgtagg gaggagaagc agaggcagga
     3901 gttggagaag acccgtcgca agctggaggg agactccaca gacctcagtg accagattgc
     3961 tgagctccag gcgcagatag cagagctcaa gatgcagctg gccaagaagg aggaggagtt
     4021 gcaggctgcc ttggccagag tggaagaaga agctgctcag aagaatatgg ccctgaagaa
     4081 gatccgagaa ctggaaactc agatatctga gctccaggag gacctggagt cggagcgagc
     4141 ctccaggaat aaagccgaga agcagaaacg ggatctggga gaggagctgg aggcgctgaa
     4201 gacagagctg gaggacacgc tggactccac ggctgcccag caggagctga ggtcgaagcg
     4261 tgagcaggag gtgagcatcc tgaagaagac tctggaggac gaggccaaga cccatgaggc
     4321 ccagatccag gagatgaggc agaagcactc acaggctgtg gaggagctgg cagatcagtt
     4381 ggagcagacg aagcgggtaa aagctaccct tgagaaggcg aagcagaccc tggagaatga
     4441 gcggggagag ctggccaatg aggtgaaggc cctgctgcaa ggcaagggcg actcagagca
     4501 caagcgcaag aaggtggagg cgcagctgca agaactgcag gtcaagttca gcgagggaga
     4561 gcgcgtgcga accgaactgg ccgacaaggt caccaagctg caggttgaac tggacagtgt
     4621 gaccggtctc cttagccagt ctgacagcaa gtccagcaag cttacgaagg acttctctgc
     4681 gctggagtcc cagcttcagg acacacagga gttgctccag gaggagaacc ggcagaagct
     4741 gagcctgagc accaagctca agcagatgga ggatgagaaa aactccttca gggagcagct
     4801 ggaggaggag gaggaggcca agcgcaactt ggagaagcag atcgccacgc tccatgccca
     4861 ggtgaccgac atgaagaaga agatggagga cggtgtaggg tgcctggaga ctgcagagga
     4921 ggcgaagcgg aggcttcaga aggacttgga aggcctgagc cagcggcttg aggagaaggt
     4981 ggctgcctac gataagctgg agaagaccaa gacacggctg cagcaggagc tggacgacct
     5041 gctggttgac ctggaccacc agcggcagag cgtctccaac ctggaaaaga agcagaagaa
     5101 gttcgaccag ctcctagccg aggagaagac catctcggcc aagtatgcag aggagcgtga
     5161 ccgagctgag gctgaggccc gtgagaagga gacaaaggcg ctatcactgg cccgggcgct
     5221 tgaggaggcc atggagcaga aggcagagct ggagcggctc aacaagcagt tccgcacgga
     5281 gatggaggac ctcatgagct ccaaggatga cgtgggcaag agtgtccacg agctggagaa
     5341 gtccaagcgg gccttggagc agcaggtgga ggagatgaag acccagctgg aggagctgga
     5401 ggatgagctg caggccacgg aggatgccaa gctccgcctg gaggtgaacc tgcaggccat
     5461 gaaggcccag tttgagcggg atctgcaggg ccgggatgaa cagagcgagg agaagaagaa
     5521 gcagctggtc agacaggtgc gggagatgga ggcggagctg gaggatgaga ggaagcagcg
     5581 ctccatggcc atggccgcac gcaagaaact ggagatggat ctgaaggacc tggaggcaca
     5641 cattgacaca gccaataaga accgggaaga ggccatcaaa cagctgcgga agcttcaggc
     5701 ccagatgaag gactgcatgc gggagctgga cgacacgcgc gcctcccggg aggagatcct
     5761 ggcgcaggcc aaggagaatg agaagaagct gaagagcatg gaggccgaga tgattcagct
     5821 gcaggaggaa ctggcagctg ctgagcgtgc taagcgtcag gcccaacagg aacgggacga
     5881 gctggctgat gagatcgcca acagcagtgg caaaggggcc ctagcattag aggagaagcg
     5941 gcgactggag gcccgcattg ccctgctgga ggaggagctg gaggaggaac agggcaacac
     6001 ggagctgatc aacgatcggc tgaagaaggc caacctgcag atcgaccaaa taaacaccga
     6061 cctgaacctg gaacgcagcc acgcacagaa gaatgagaat gcgcgacagc agctggaacg
     6121 ccagaacaag gagctcaagg ccaagctgca ggaaatggag agtgctgtca agtccaaata
     6181 caaggcctcc atcgcggcct tggaggccaa aattgcacag ctggaggaac agctggacaa
     6241 cgagaccaag gagcgccagg cagcctccaa gcaggtgcgc cggacggaga agaagctgaa
     6301 ggacgtgctg ctgcaggtgg aggacgagcg gaggaacgcg gaacagttca aggaccaggc
     6361 tgacaaggcg tccacccgcc tgaagcagct taaacggcag ctagaggagg ctgaagagga
     6421 ggcccagcgg gccaatgcct cacgccggaa gctgcagcgt gagctggaag atgccacaga
     6481 gaccgctgat gctatgaacc gcgaggtcag ctccctgaag aacaaactga ggcgtgggga
     6541 cctgccattt gtcgtgactc gccgaattgt tcggaaaggc actggcgact gctcagacga
     6601 ggaggtcgac ggtaaagcag atggggccga tgccaaggca gctgaatagg aattctgcag
     6661 tcgacggtac cgcgggcccg ggatccaccg gatctagata actgatcata atcagccata
     6721 ccac
//
