LOCUS       sequence-86899-e         978 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 590, Emission: 649.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 978)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mPlum-Paxillin-22
FEATURES             Location/Qualifiers
     source          1..978
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    193..245
                     /label=MCS
                     /note="multiple cloning site"
     CDS             259..939
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     660..679
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     854..877
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 cacgagcgcc gcggctcgct gtgctctggc tgccagaagc ccatcacggg acgctgcatc
       61 actgccatgg gcaagaaatt tcaccccgaa cactttgtct gtgccttctg cctcaagcag
      121 ctcaacaaag gaaccttcaa agaacagaac gacaagccct actgtcagaa ctgctttctc
      181 aaactcttct gtctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg
      241 gatccaccgg tggctaccat ggtgagcaag ggcgaggagg tcatcaagga gttcatgcgc
      301 ttcaaggagc acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc
      361 gagggccgcc cctacgaggg cacccagacc gccaggctga aggtgaccaa gggtggcccc
      421 ctgcccttcg cctgggacat cctgtcccct cagatcatgt acggctccaa ggcctacgtg
      481 aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg
      541 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg
      601 caggacggcg agttcatcta caaggtgaag gtgcgcggca ccaacttccc ctccgacggc
      661 cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag
      721 gacggcgccc tgaagggcga gatgaagatg aggctgaggc tgaaggacgg cggccactac
      781 gacgccgagg tcaagaccac ctacatggcc aagaagcccg tgcagctgcc cggcgcctac
      841 aagaccgaca tcaagctgga catcacctcc cacaacgagg actacaccat cgtggaacag
      901 tacgagcgcg ccgagggccg ccactccacc ggcgcctaag cggccgcgac tctagatcat
      961 aatcagccat accacatt
//
