LOCUS       sequence-86900-e        1676 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus, Excitation: 590, Emission: 649.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1676)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mPlum-PCNA-19-SV40NLS-4
FEATURES             Location/Qualifiers
     source          1..1676
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             28..48
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             52..72
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             76..96
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             109..786
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     510..529
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     704..727
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 gacgtcagat ccgctagccc catggatcca aaaaagaaga gaaaggtaga tccaaaaaag
       61 aagagaaagg tagatccaaa aaagaagaga aaggtaccgg tggctaccat ggtgagcaag
      121 ggcgaggagg tcatcaagga gttcatgcgc ttcaaggagc acatggaggg ctccgtgaac
      181 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
      241 gccaggctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct
      301 cagatcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg
      361 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc
      421 gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag
      481 gtgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg
      541 gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatgaagatg
      601 aggctgaggc tgaaggacgg cggccactac gacgccgagg tcaagaccac ctacatggcc
      661 aagaagcccg tgcagctgcc cggcgcctac aagaccgaca tcaagctgga catcacctcc
      721 cacaacgagg actacaccat cgtggaacag tacgagcgcg ccgagggccg ccactccacc
      781 ggcgcctccg gagaagggca agggcaaggg caagggccgg gccgcggcta cgcgtatcga
      841 tccatgttcg aggcgcgcct ggtccagggc tccatcctca agaaggtgtt ggaggcactc
      901 aaggacctca tcaacgaggc ctgctgggat attagctcca gcggtgtaaa cctgcagagc
      961 atggactcgt cccacgtctc tttggtgcag ctcaccctgc ggtctgaggg cttcgacacc
     1021 taccgctgcg accgcaacct ggccatgggc gtgaacctca ccagtatgtc caaaatacta
     1081 aaatgcgccg gcaatgaaga tatcattaca ctaagggccg aagataacgc ggataccttg
     1141 gcgctagtat ttgaagcacc aaaccaggag aaagtttcag actatgaaat gaagttgatg
     1201 gatttagatg ttgaacaact tggaattcca gaacaggagt acagctgtgt agtaaagatg
     1261 ccttctggtg aatttgcacg tatatgccga gatctcagcc atattggaga tgctgttgta
     1321 atttcctgtg caaaagacgg agtgaaattt tctgcaagtg gagaacttgg aaatggaaac
     1381 attaaattgt cacagacaag taatgtcgat aaagaggagg aagctgttac catagagatg
     1441 aatgaaccag ttcaactaac ttttgcactg aggtacctga acttctttac aaaagccact
     1501 ccactctctt caacggtgac actcagtatg tctgcagatg taccccttgt tgtagagtat
     1561 aaaattgcgg atatgggaca cttaaaatac tacttggctc ccaagatcga ggatgaagaa
     1621 ggatcttagg gatccaccgg atctagataa ctgatcataa tcagccatac cacatt
//
