LOCUS       sequence-86901-e        2601 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Centrosomes, Excitation: 590, Emission: 649.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2601)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mPlum-PLK1-N-16
FEATURES             Location/Qualifiers
     source          1..2601
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1876..1885
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1882..2562
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     2283..2302
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     2477..2500
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc caccatgagt gctgcagtga ctgcagggaa gctggcacgg
       61 gcaccggccg accctgggaa agccggggtc cccggagttg cagctcccgg agctccggcg
      121 gcggctccac cggcgaaaga gatcccggag gtcctagtgg acccacgcag ccggcggcgc
      181 tatgtgcggg gccgcttttt gggcaagggc ggctttgcca agtgcttcga gatctcggac
      241 gcggacacca aggaggtgtt cgcgggcaag attgtgccta agtctctgct gctcaagccg
      301 caccagaggg agaagatgtc catggaaata tccattcacc gcagcctcgc ccaccagcac
      361 gtcgtaggat tccacggctt tttcgaggac aacgacttcg tgttcgtggt gttggagctc
      421 tgccgccgga ggtctctcct ggagctgcac aagaggagga aagccctgac tgagcctgag
      481 gcccgatact acctacggca aattgtgctt ggctgccagt acctgcaccg aaaccgagtt
      541 attcatcgag acctcaagct gggcaacctt ttcctgaatg aagatctgga ggtgaaaata
      601 ggggattttg gactggcaac caaagtcgaa tatgacgggg agaggaagaa gaccctgtgt
      661 gggactccta attacatagc tcccgaggtg ctgagcaaga aagggcacag tttcgaggtg
      721 gatgtgtggt ccattgggtg tatcatgtat accttgttag tgggcaaacc accttttgag
      781 acttcttgcc taaaagagac ctacctccgg atcaagaaga atgaatacag tattcccaag
      841 cacatcaacc ccgtggccgc ctccctcatc cagaagatgc ttcagacaga tcccactgcc
      901 cgcccaacca ttaacgagct gcttaatgac gagttcttta cttctggcta tatccctgcc
      961 cgtctcccca tcacctgcct gaccattcca ccaaggtttt cgattgctcc cagcagcctg
     1021 gaccccagca accggaagcc cctcacagtc ctcaataaag gcttggagaa ccccctgcct
     1081 gagcgtcccc gggaaaaaga agaaccagtg gttcgagaga caggtgaggt ggtcgactgc
     1141 cacctcagtg acatgctgca gcagctgcac agtgtcaatg cctccaagcc ctcggagcgt
     1201 gggctggtca ggcaagagga ggctgaggat cctgcctgca tccccatctt ctgggtcagc
     1261 aagtgggtgg actattcgga caagtacggc cttgggtatc agctctgtga taacagcgtg
     1321 ggggtgctct tcaatgactc aacacgcctc atcctctaca atgatggtga cagcctgcag
     1381 tacatagagc gtgacggcac tgagtcctac ctcaccgtga gttcccatcc caactccttg
     1441 atgaagaaga tcaccctcct taaatatttc cgcaattaca tgagcgagca cttgctgaag
     1501 gcaggtgcca acatcacgcc gcgcgaaggt gatgagctcg cccggctgcc ctacctacgg
     1561 acctggttcc gcacccgcag cgccatcatc ctgcacctca gcaacggcag cgtgcagatc
     1621 aacttcttcc aggatcacac caagctcatc ttgtgcccac tgatggcagc cgtgacctac
     1681 atcgacgaga agcgggactt ccgcacatac cgcctgagtc tcctggagga gtacggctgc
     1741 tgcaaggagc tggccagccg gctccgctac gcccgcacta tggtggacaa gctgctgagc
     1801 tcacgctcgg ccagcaaccg tctcaaggcc tccggtagcg gcagcgcatc cggcggaagc
     1861 ggaagcggac cggtcgccac catggtgagc aagggcgagg aggtcatcaa ggagttcatg
     1921 cgcttcaagg agcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
     1981 ggcgagggcc gcccctacga gggcacccag accgccaggc tgaaggtgac caagggtggc
     2041 cccctgccct tcgcctggga catcctgtcc cctcagatca tgtacggctc caaggcctac
     2101 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag
     2161 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
     2221 ctgcaggacg gcgagttcat ctacaaggtg aaggtgcgcg gcaccaactt cccctccgac
     2281 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc
     2341 gaggacggcg ccctgaaggg cgagatgaag atgaggctga ggctgaagga cggcggccac
     2401 tacgacgccg aggtcaagac cacctacatg gccaagaagc ccgtgcagct gcccggcgcc
     2461 tacaagaccg acatcaagct ggacatcacc tcccacaacg aggactacac catcgtggaa
     2521 cagtacgagc gcgccgaggg ccgccactcc accggcgcct aagcggccgc gactctagat
     2581 cataatcagc cataccacat t
//
