LOCUS       sequence-86902-e        1428 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Ras GTPase, Excitation: 590, Emission: 649.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1428)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 1428)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mPlum-Rab5a-7
FEATURES             Location/Qualifiers
     source          1..1428
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             35..712
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     436..455
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     630..653
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtggc taccatggtg agcaagggcg aggaggtcat
       61 caaggagttc atgcgcttca aggagcacat ggagggctcc gtgaacggcc acgagttcga
      121 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca ggctgaaggt
      181 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcaga tcatgtacgg
      241 ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc
      301 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac
      361 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaaggtgc gcggcaccaa
      421 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga
      481 gcggatgtac cccgaggacg gcgccctgaa gggcgagatg aagatgaggc tgaggctgaa
      541 ggacggcggc cactacgacg ccgaggtcaa gaccacctac atggccaaga agcccgtgca
      601 gctgcccggc gcctacaaga ccgacatcaa gctggacatc acctcccaca acgaggacta
      661 caccatcgtg gaacagtacg agcgcgccga gggccgccac tccaccggcg cctccggact
      721 cagatctcga gccatggcta gtcgaggcgc aacaagaccc aacgggccaa atactggaaa
      781 taaaatatgc cagttcaaac tagtacttct gggagagtct gctgttggca aatcaagcct
      841 agtgcttcgt tttgtgaagg gccaatttca tgaatttcaa gagagtacca taggggctgc
      901 ttttctaacc caaactgtgt gtcttgatga tacaacagta aagtttgaaa tatgggatac
      961 agctggtcaa gaacgatacc atagcttagc accaatgtac tacagaggag cacaagcagc
     1021 catagttgta tatgatatca caaatgagga gtcctttgcc agagccaaaa actgggttaa
     1081 agaacttcag aggcaagcca gtcctaacat tgtaatagct ttatcaggaa acaaggctga
     1141 tcttgcaaat aaaagagctg tcgatttcca ggaagcacag tcctatgcag atgacaacag
     1201 tttattattc atggagacat cagctaaaac atcgatgaat gtaaatgaaa tattcatggc
     1261 aatagctaaa aagttgccaa agaacgaacc acagaatcca ggagcaaatt ctgccagagg
     1321 aagaggagta gaccttactg aacccacgca gccaaccagg aatcagtgtt gtagtaacta
     1381 aggatccacc ggatctagat aactgatcat aatcagccat accacatt
//
