LOCUS       sequence-86911-e        1892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin/Focal Adhesions, Excitation: 590, Emission: 649.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1892)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mPlum-VASP-5
FEATURES             Location/Qualifiers
     source          1..1892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             33..710
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     434..453
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     628..651
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 cgtcagatcc gctagcgcta ccggtggcta ccatggtgag caagggcgag gaggtcatca
       61 aggagttcat gcgcttcaag gagcacatgg agggctccgt gaacggccac gagttcgaga
      121 tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccagg ctgaaggtga
      181 ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagatc atgtacggct
      241 ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg
      301 agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc
      361 aggactcctc cctgcaggac ggcgagttca tctacaaggt gaaggtgcgc ggcaccaact
      421 tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc
      481 ggatgtaccc cgaggacggc gccctgaagg gcgagatgaa gatgaggctg aggctgaagg
      541 acggcggcca ctacgacgcc gaggtcaaga ccacctacat ggccaagaag cccgtgcagc
      601 tgcccggcgc ctacaagacc gacatcaagc tggacatcac ctcccacaac gaggactaca
      661 ccatcgtgga acagtacgag cgcgccgagg gccgccactc caccggcgcc tccggactca
      721 gatctatgag cgagacggtc atctgttcca gccgggctac tgtgatgctt tatgatgaca
      781 gcaacaagcg atggctccct gctggcactg gtccccaggc cttcagccgc gtccagatat
      841 accacaaccc cactgctaac tccttccgag ttgttggccg caagatgcag ccggatcagc
      901 aggtggttat caactgtgcc atcattcggg gtgtcaagta caatcaggcc actcccatct
      961 tccatcagtg gcgagatgcc cgccaggtct ggggcctcaa cttcggcagc aaggaggacg
     1021 ccatacagtt tgcaacaggc atggccaacg ccctagaggc cttggaagga ggtgggcctc
     1081 ccccagcccc agcaccccct gcctggtctg cccagaatgg tccctcccca gaggagctgg
     1141 aacaacagaa aaggcagccg gagcatatgg agcgccgggt ctccaatgca ggaggcccac
     1201 ctgctccccc agctgggggc cctcctccac ctccaggacc tccccctcct ccaggtcccc
     1261 ccccaccccc aggtctgccc tcctcagggg tatctggggc aggtcatgga gcaggggcag
     1321 ccccaccccc tgcaccccca ctccctacaa cacagggccc caatagtggg ggttccgggg
     1381 ccccaggcct ggctgctgcc attgctggag ccaaactcag gaaagtgagc aagcaggagg
     1441 aggcctctgg ggggcccctg gcccccaaag ctgagaacag tcgaagcact ggtggggggc
     1501 ttatggaaga gatgaacgcc atgctggccc ggagaagaaa agccacacag gttggggaga
     1561 agccccccaa agacgagtca gccagtgagg agtcagaggc ccgactccct gcccagagtg
     1621 aacctgtgag aagaccctgg gagaagaaca gcacaacctt gccaaggatg aagtcgtcct
     1681 cttctgtgac tacctccgag gcccacccct cgacgccctg ctccagtgat gactccgact
     1741 tggagagggt gaagcaggag cttctggaag aggtgcggaa ggagctacag aaaatgaaag
     1801 aggaaatcat cgaagtcttt gtccaggagc tgaggaagcg gggttctcct tgagcggccg
     1861 cgactctaga tcataatcag ccataccaca tt
//
