LOCUS       sequence-86913-e        2190 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Intermediate Filaments, Excitation: 590, Emission: 
            649.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2190)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mPlum-Vimentin-7
FEATURES             Location/Qualifiers
     source          1..2190
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1471..2151
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     1872..1891
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     2066..2089
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 gatccgctag cgctaccgga ctcagatctc gagctcaagc ttcgaattcc catgtccacc
       61 aggtccgtgt cctcgtcctc ctaccgcagg atgttcggcg gcccgggcac cgcgagccgg
      121 ccgagctcca gccggagcta cgtgactacg tccacccgca cctacagcct gggcagcgcg
      181 ctgcgcccca gcaccagccg cagcctctac gcctcgtccc cgggcggcgt gtatgccacg
      241 cgctcctctg ccgtgcgcct gcggagcagc gtgcccgggg tgcggctcct gcaggactcg
      301 gtggacttct cgctggccga cgccatcaac accgagttca agaacacccg caccaacgag
      361 aaggtggagc tgcaggagct gaatgaccgc ttcgccaact acatcgacaa ggtgcgcttc
      421 ctggagcagc agaataagat cctgctggcc gagctcgagc agctcaaggg ccaaggcaag
      481 tcgcgcctgg gggacctcta cgaggaggag atgcgggagc tgcgccggca ggtggaccag
      541 ctaaccaacg acaaagcccg cgtcgaggtg gagcgcgaca acctggccga ggacatcatg
      601 cgcctccggg agaaattgca ggaggagatg cttcagagag aggaagccga aaacaccctg
      661 caatctttca gacaggatgt tgacaatgcg tctctggcac gtcttgacct tgaacgcaaa
      721 gtggaatctt tgcaagaaga gattgccttt ttgaagaaac tccacgaaga ggaaatccag
      781 gagctgcagg ctcagattca ggaacagcat gtccaaatcg atgtggatgt ttccaagcct
      841 gacctcacgg ctgccctgcg tgacgtacgt cagcaatatg aaagtgtggc tgccaagaac
      901 ctgcaggagg cagaagaatg gtacaaatcc aagtttgctg acctctctga ggctgccaac
      961 cggaacaatg acgccctgcg ccaggcaaag caggagtcca ctgagtaccg gagacaggtg
     1021 cagtccctca cctgtgaagt ggatgccctt aaaggaacca atgagtccct ggaacgccag
     1081 atgcgtgaaa tggaagagaa ctttgccgtt gaagctgcta actaccaaga cactattggc
     1141 cgcctgcagg atgagattca gaatatgaag gaggaaatgg ctcgtcacct tcgtgaatac
     1201 caagacctgc tcaatgttaa gatggccctt gacattgaga ttgccaccta caggaagctg
     1261 ctggaaggcg aggagagcag gatttctctg cctcttccaa acttttcctc cctgaacctg
     1321 agggaaacta atctggattc actccctctg gttgataccc actcaaaaag gacacttctg
     1381 attaagacgg ttgaaactag agatggacag gttatcaacg aaacttctca gcatcacgat
     1441 gaccttgaag gggatccacc ggtggctacc atggtgagca agggcgagga ggtcatcaag
     1501 gagttcatgc gcttcaagga gcacatggag ggctccgtga acggccacga gttcgagatc
     1561 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaggct gaaggtgacc
     1621 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagatcat gtacggctcc
     1681 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag
     1741 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
     1801 gactcctccc tgcaggacgg cgagttcatc tacaaggtga aggtgcgcgg caccaacttc
     1861 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg
     1921 atgtaccccg aggacggcgc cctgaagggc gagatgaaga tgaggctgag gctgaaggac
     1981 ggcggccact acgacgccga ggtcaagacc acctacatgg ccaagaagcc cgtgcagctg
     2041 cccggcgcct acaagaccga catcaagctg gacatcacct cccacaacga ggactacacc
     2101 atcgtggaac agtacgagcg cgccgagggc cgccactcca ccggcgccta agcggccgcg
     2161 actctagatc ataatcagcc ataccacatt
//
