LOCUS       sequence-86916-e        2485 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 590, Emission: 649.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2485)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mPlum-Zyxin-6
FEATURES             Location/Qualifiers
     source          1..2485
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      42..51
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1778..2458
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     2179..2198
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     2373..2396
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 tagcgctacc ggactcagat ctcgagctca agcttcgaat tccatggcgg ccccccgccc
       61 gtctcccgcg atccccgttt cggtctcggc tccggctttt tacgccccgc agaagaagtt
      121 cggccctgtg gtggccccaa agcccaaagt gaatcccttc cggcccaggg acagcgagcc
      181 tcccccggca cccggggccc agcgcgcaca gatgggccgg gtgggcgata ttcccccgcc
      241 gcccccggaa gactttcccc tgcctccacc tccccttgct ggggatggcg acgatgcaga
      301 gggtgctctg ggaggtgcct tcccgccgcc ccctcccccg atcgaggaat catttccccc
      361 tgcgcctctg gaggaggaga tcttcccttc cccgccgcct cctccggagg aggagggagg
      421 gcctgaggcc cccataccgc ccccaccaca gcccagggag aaggtgagca gtattgattt
      481 ggagatcgac tctctgtcct cactgctgga tgacatgacc aagaatgatc ctttcaaagc
      541 ccgggtgtca tctggatatg tgcccccacc agtggccact ccattcagtt ccaagtccag
      601 taccaagcct gcagccgggg gcacagcacc cctgcctcct tggaagtccc cttccagctc
      661 ccagcctctg ccccaggttc cggctccggc tcagagccag acacagttcc atgttcagcc
      721 ccagccccag cccaagcctc aggtccaact ccatgtccag tcccagaccc agcctgtgtc
      781 tttggctaac acccagcccc gagggccccc agcctcatct ccggctccag cccctaagtt
      841 ttctccagtg actcctaagt ttactcctgt ggcttccaag ttcagtcctg gagccccagg
      901 tggatctggg tcacaaccaa atcaaaaatt ggggcacccc gaagctcttt ctgctggcac
      961 aggctcccct caacctccca gcttcaccta tgcccagcag agggagaagc cccgagtgca
     1021 ggagaagcag caccccgtgc ccccaccggc tcagaaccaa aaccaggtgc gctcccctgg
     1081 ggccccaggg cccctgactc tgaaggaggt ggaggagctg gagcagctga cccagcagct
     1141 aatgcaggac atggagcatc ctcagaggca gaatgtggct gtcaacgaac tctgcggccg
     1201 atgccatcaa cccctggccc gggcgcagcc agccgtccgc gctctagggc agctgttcca
     1261 catcgcctgc ttcacctgcc accagtgtgc gcagcagctc cagggccagc agttctacag
     1321 tctggagggg gcgccgtact gcgagggctg ttacactgac accctggaga agtgtaacac
     1381 ctgcggggag cccatcactg accgcatgct gagggccacg ggcaaggcct atcacccgca
     1441 ctgcttcacc tgtgtggtct gcgcccgccc cctggagggc acctccttca tcgtggacca
     1501 ggccaaccgg ccccactgtg tccccgacta ccacaagcag tacgccccga ggtgctccgt
     1561 ctgctctgag cccatcatgc ctgagcctgg ccgagatgag actgtgcgag tggtcgccct
     1621 ggacaagaac ttccacatga agtgttacaa gtgtgaggac tgcgggaagc ccctgtcgat
     1681 tgaggcagat gacaatggct gcttccccct ggacggtcac gtgctctgtc ggaagtgcca
     1741 cactgctaga gcccagacgg atccaccggt ggctaccatg gtgagcaagg gcgaggaggt
     1801 catcaaggag ttcatgcgct tcaaggagca catggagggc tccgtgaacg gccacgagtt
     1861 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaggctgaa
     1921 ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agatcatgta
     1981 cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt
     2041 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt
     2101 gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagg tgcgcggcac
     2161 caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc
     2221 cgagcggatg taccccgagg acggcgccct gaagggcgag atgaagatga ggctgaggct
     2281 gaaggacggc ggccactacg acgccgaggt caagaccacc tacatggcca agaagcccgt
     2341 gcagctgccc ggcgcctaca agaccgacat caagctggac atcacctccc acaacgagga
     2401 ctacaccatc gtggaacagt acgagcgcgc cgagggccgc cactccaccg gcgcctaagc
     2461 ggccgcgact ctagatcata atcag
//
