LOCUS       sequence-86941-e        2130 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Centromeres, Excitation: 586, Emission: 632.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Shcherbo D, Murphy CS, Ermakova GV, Solovieva EA, Chepurnykh TV, 
            Shcheglov AS, Verkhusha VV, Pletnev VZ, Hazelwood KL, Roche PM, 
            Lukyanov S, Zaraisky AG, Davidson MW, Chudakov DM
  TITLE     Far-red fluorescent tags for protein imaging in living tissues.
  JOURNAL   Biochem J. 2009 Mar 15;418(3):567-74. doi: 10.1042/BJ20081949.
  PUBMED    19143658
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Biochem J"; date: "2009-03-15- 15"; volume: "418"; issue: "3"; 
            pages: "567-74"
COMMENT     Sequence Label: tdKatushka2-CENPB-N-22
FEATURES             Location/Qualifiers
     source          1..2130
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    531..583
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      591..600
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             597..1301
                     /codon_start=1
                     /product="improved version of the oligomeric far-red 
                     fluorescent protein TurboFP635/Katushka (Luker et al., 
                     2015)"
                     /label=Katushka2S
                     /note="mammalian codon-optimized"
                     /translation="MVGEDSVLITENMHMKLYMEGTVNDHHFKCTSEGEGKPYEGTQTM
                     KIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERITTYEDGG
                     VLTATQDTSLQNGCLIYNVKINGVNFPSNGPVMQKKTLGWEASTEMLYPADSGLRGHAQ
                     MALKLVGGGYLHCSLKTTYRSKKPAKNLKMPGFYFVDRRLERIKEADKETYVEQHEMAV
                     ARYCDLPSKLGHS"
     CDS             597..1301
                     /codon_start=1
                     /product="far-red-shifted derivative of red fluorescent 
                     protein from Entacmaea quadricolor, also known as Katushka 
                     (Shcherbo et al., 2007)"
                     /label=TurboFP635
                     /note="mammalian codon-optimized"
                     /translation="MVGEDSVLITENMHMKLYMEGTVNDHHFKCTSEGEGKPYEGTQTM
                     KIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERITTYEDGG
                     VLTATQDTSLQNGCLIYNVKINGVNFPSNGPVMQKKTLGWEASTEMLYPADSGLRGHAQ
                     MALKLVGGGYLHCSLKTTYRSKKPAKNLKMPGFYFVDRRLERIKEADKETYVEQHEMAV
                     ARYCDLPSKLGHS"
     CDS             1371..2075
                     /codon_start=1
                     /product="improved version of the oligomeric far-red 
                     fluorescent protein TurboFP635/Katushka (Luker et al., 
                     2015)"
                     /label=Katushka2S
                     /note="mammalian codon-optimized"
                     /translation="MVGEDSVLITENMHMKLYMEGTVNDHHFKCTSEGEGKPYEGTQTM
                     KIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERITTYEDGG
                     VLTATQDTSLQNGCLIYNVKINGVNFPSNGPVMQKKTLGWEASTEMLYPADSGLRGHAQ
                     MALKLVGGGYLHCSLKTTYRSKKPAKNLKMPGFYFVDRRLERIKEADKETYVEQHEMAV
                     ARYCDLPSKLGHS"
     CDS             1371..2075
                     /codon_start=1
                     /product="far-red-shifted derivative of red fluorescent 
                     protein from Entacmaea quadricolor, also known as Katushka 
                     (Shcherbo et al., 2007)"
                     /label=TurboFP635
                     /note="mammalian codon-optimized"
                     /translation="MVGEDSVLITENMHMKLYMEGTVNDHHFKCTSEGEGKPYEGTQTM
                     KIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERITTYEDGG
                     VLTATQDTSLQNGCLIYNVKINGVNFPSNGPVMQKKTLGWEASTEMLYPADSGLRGHAQ
                     MALKLVGGGYLHCSLKTTYRSKKPAKNLKMPGFYFVDRRLERIKEADKETYVEQHEMAV
                     ARYCDLPSKLGHS"
ORIGIN
        1 cgacgtcaga tccgctagcc accatgggcc ccaagcggcg gcagctgacg ttccgggaga
       61 agtcgcggat catccaggag gtggaggaga acccggacct acgcaagggc gagatcgcgc
      121 ggcgcttcaa catcccgccg tccacgctga gcacgatcct gaagaacaag cgcgccatcc
      181 tggcgtcgga gcgcaagtac ggggtggcct ccacctgccg caagaccaac aagctgtctc
      241 cctacgacaa gctcgagggc ttgctcatcg cctggttcca gcagatccgc gccgccggcc
      301 tgccggtcaa gggcatcatc ctcaaggaga aggcgctgcg catagccgag gagctgggca
      361 tggacgactt caccgcctcc aacggctggc tggaccgctt ccgccggcgc cacggcgtgg
      421 tgtcctgcag cggcgtggcc cgcgcccgcg cgcgaaacgc tgccccccgc accccggcgg
      481 cgcctgccag tccggccgcg gtgccctcgg agggcagtgg cgggagcact ctcgagctca
      541 agcttcgaat tctgcagtcg acggtaccgc gggcccggga tccaccggtc gccaccatgg
      601 tgggtgagga tagcgtgctg atcaccgaga acatgcacat gaaactgtac atggagggca
      661 ccgtgaacga ccaccacttc aagtgcacat ccgagggcga aggcaagccc tacgagggca
      721 cccagaccat gaagatcaag gtggtcgagg gcggccctct ccccttcgcc ttcgacatcc
      781 tggctaccag cttcatgtac ggcagcaaaa cctttatcaa ccacacccag ggcatccccg
      841 acttctttaa gcagtccttc cctgagggct tcacatggga gaggatcacc acatacgaag
      901 acgggggcgt gctgaccgct acccaggaca ccagcctcca gaacggctgc ctcatctaca
      961 acgtcaagat caacggggtg aacttcccat ccaacggccc tgtgatgcag aagaaaacac
     1021 tcggctggga ggccagcacc gagatgctgt accccgctga cagcggcctg agaggccatg
     1081 cccagatggc cctgaagctc gtgggcgggg gctacctgca ctgctccctc aagaccacat
     1141 acagatccaa gaaacccgct aagaacctca agatgcccgg cttctacttc gtggacagga
     1201 gactggaaag aatcaaggag gccgacaaag agacctacgt cgagcagcac gagatggctg
     1261 tggccaggta ctgcgacctg cctagcaaac tggggcacag cgggcatggc accggcagca
     1321 ccggcagcgg cagctccggt accgcctcct ccgaggacaa caacatggcc atggtgggtg
     1381 aggatagcgt gctgatcacc gagaacatgc acatgaaact gtacatggag ggcaccgtga
     1441 acgaccacca cttcaagtgc acatccgagg gcgaaggcaa gccctacgag ggcacccaga
     1501 ccatgaagat caaggtggtc gagggcggcc ctctcccctt cgccttcgac atcctggcta
     1561 ccagcttcat gtacggcagc aaaaccttta tcaaccacac ccagggcatc cccgacttct
     1621 ttaagcagtc cttccctgag ggcttcacat gggagaggat caccacatac gaagacgggg
     1681 gcgtgctgac cgctacccag gacaccagcc tccagaacgg ctgcctcatc tacaacgtca
     1741 agatcaacgg ggtgaacttc ccatccaacg gccctgtgat gcagaagaaa acactcggct
     1801 gggaggccag caccgagatg ctgtaccccg ctgacagcgg cctgagaggc catgcccaga
     1861 tggccctgaa gctcgtgggc gggggctacc tgcactgctc cctcaagacc acatacagat
     1921 ccaagaaacc cgctaagaac ctcaagatgc ccggcttcta cttcgtggac aggagactgg
     1981 aaagaatcaa ggaggccgac aaagagacct acgtcgagca gcacgagatg gctgtggcca
     2041 ggtactgcga cctgcctagc aaactggggc acagctccgg actcagatct tgagcggccg
     2101 cgactctaga tcataatcag ccataccaca
//
