LOCUS       sequence-86945-e        1774 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 586, Emission: 632.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Shcherbo D, Murphy CS, Ermakova GV, Solovieva EA, Chepurnykh TV, 
            Shcheglov AS, Verkhusha VV, Pletnev VZ, Hazelwood KL, Roche PM, 
            Lukyanov S, Zaraisky AG, Davidson MW, Chudakov DM
  TITLE     Far-red fluorescent tags for protein imaging in living tissues.
  JOURNAL   Biochem J. 2009 Mar 15;418(3):567-74. doi: 10.1042/BJ20081949.
  PUBMED    19143658
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Biochem J"; date: "2009-03-15- 15"; volume: "418"; issue: "3"; 
            pages: "567-74"
COMMENT     Sequence Label: tdKatushka2-CytERM-N-17
FEATURES             Location/Qualifiers
     source          1..1774
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      222..231
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             228..932
                     /codon_start=1
                     /product="improved version of the oligomeric far-red 
                     fluorescent protein TurboFP635/Katushka (Luker et al., 
                     2015)"
                     /label=Katushka2S
                     /note="mammalian codon-optimized"
                     /translation="MVGEDSVLITENMHMKLYMEGTVNDHHFKCTSEGEGKPYEGTQTM
                     KIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERITTYEDGG
                     VLTATQDTSLQNGCLIYNVKINGVNFPSNGPVMQKKTLGWEASTEMLYPADSGLRGHAQ
                     MALKLVGGGYLHCSLKTTYRSKKPAKNLKMPGFYFVDRRLERIKEADKETYVEQHEMAV
                     ARYCDLPSKLGHS"
     CDS             228..932
                     /codon_start=1
                     /product="far-red-shifted derivative of red fluorescent 
                     protein from Entacmaea quadricolor, also known as Katushka 
                     (Shcherbo et al., 2007)"
                     /label=TurboFP635
                     /note="mammalian codon-optimized"
                     /translation="MVGEDSVLITENMHMKLYMEGTVNDHHFKCTSEGEGKPYEGTQTM
                     KIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERITTYEDGG
                     VLTATQDTSLQNGCLIYNVKINGVNFPSNGPVMQKKTLGWEASTEMLYPADSGLRGHAQ
                     MALKLVGGGYLHCSLKTTYRSKKPAKNLKMPGFYFVDRRLERIKEADKETYVEQHEMAV
                     ARYCDLPSKLGHS"
     CDS             1002..1706
                     /codon_start=1
                     /product="improved version of the oligomeric far-red 
                     fluorescent protein TurboFP635/Katushka (Luker et al., 
                     2015)"
                     /label=Katushka2S
                     /note="mammalian codon-optimized"
                     /translation="MVGEDSVLITENMHMKLYMEGTVNDHHFKCTSEGEGKPYEGTQTM
                     KIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERITTYEDGG
                     VLTATQDTSLQNGCLIYNVKINGVNFPSNGPVMQKKTLGWEASTEMLYPADSGLRGHAQ
                     MALKLVGGGYLHCSLKTTYRSKKPAKNLKMPGFYFVDRRLERIKEADKETYVEQHEMAV
                     ARYCDLPSKLGHS"
     CDS             1002..1706
                     /codon_start=1
                     /product="far-red-shifted derivative of red fluorescent 
                     protein from Entacmaea quadricolor, also known as Katushka 
                     (Shcherbo et al., 2007)"
                     /label=TurboFP635
                     /note="mammalian codon-optimized"
                     /translation="MVGEDSVLITENMHMKLYMEGTVNDHHFKCTSEGEGKPYEGTQTM
                     KIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERITTYEDGG
                     VLTATQDTSLQNGCLIYNVKINGVNFPSNGPVMQKKTLGWEASTEMLYPADSGLRGHAQ
                     MALKLVGGGYLHCSLKTTYRSKKPAKNLKMPGFYFVDRRLERIKEADKETYVEQHEMAV
                     ARYCDLPSKLGHS"
ORIGIN
        1 gccatctagt gacgtcagat ccgctagcgc taccggactc agatctggta ccccttgcag
       61 cttccagttg tcaggaagag aaggctccca tggatcctgt ggtggtgctg gggctctgtc
      121 tctcctgttt gcttctcctt tcactctgga aacagagcta tgggggaggg aagcttcgaa
      181 ttctgcagtc gacggtaccg cgggcccggg atccaccggt cgccaccatg gtgggtgagg
      241 atagcgtgct gatcaccgag aacatgcaca tgaaactgta catggagggc accgtgaacg
      301 accaccactt caagtgcaca tccgagggcg aaggcaagcc ctacgagggc acccagacca
      361 tgaagatcaa ggtggtcgag ggcggccctc tccccttcgc cttcgacatc ctggctacca
      421 gcttcatgta cggcagcaaa acctttatca accacaccca gggcatcccc gacttcttta
      481 agcagtcctt ccctgagggc ttcacatggg agaggatcac cacatacgaa gacgggggcg
      541 tgctgaccgc tacccaggac accagcctcc agaacggctg cctcatctac aacgtcaaga
      601 tcaacggggt gaacttccca tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg
      661 aggccagcac cgagatgctg taccccgctg acagcggcct gagaggccat gcccagatgg
      721 ccctgaagct cgtgggcggg ggctacctgc actgctccct caagaccaca tacagatcca
      781 agaaacccgc taagaacctc aagatgcccg gcttctactt cgtggacagg agactggaaa
      841 gaatcaagga ggccgacaaa gagacctacg tcgagcagca cgagatggct gtggccaggt
      901 actgcgacct gcctagcaaa ctggggcaca gcgggcatgg caccggcagc accggcagcg
      961 gcagctccgg taccgcctcc tccgaggaca acaacatggc catggtgggt gaggatagcg
     1021 tgctgatcac cgagaacatg cacatgaaac tgtacatgga gggcaccgtg aacgaccacc
     1081 acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag accatgaaga
     1141 tcaaggtggt cgagggcggc cctctcccct tcgccttcga catcctggct accagcttca
     1201 tgtacggcag caaaaccttt atcaaccaca cccagggcat ccccgacttc tttaagcagt
     1261 ccttccctga gggcttcaca tgggagagga tcaccacata cgaagacggg ggcgtgctga
     1321 ccgctaccca ggacaccagc ctccagaacg gctgcctcat ctacaacgtc aagatcaacg
     1381 gggtgaactt cccatccaac ggccctgtga tgcagaagaa aacactcggc tgggaggcca
     1441 gcaccgagat gctgtacccc gctgacagcg gcctgagagg ccatgcccag atggccctga
     1501 agctcgtggg cgggggctac ctgcactgct ccctcaagac cacatacaga tccaagaaac
     1561 ccgctaagaa cctcaagatg cccggcttct acttcgtgga caggagactg gaaagaatca
     1621 aggaggccga caaagagacc tacgtcgagc agcacgagat ggctgtggcc aggtactgcg
     1681 acctgcctag caaactgggg cacagctccg gactcagatc ttgagcggcc gcgactctag
     1741 atcataatca gccataccac attgtagagt tgcc
//
