LOCUS       sequence-86948-e        2547 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleoli, Excitation: 586, Emission: 632.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Shcherbo D, Murphy CS, Ermakova GV, Solovieva EA, Chepurnykh TV, 
            Shcheglov AS, Verkhusha VV, Pletnev VZ, Hazelwood KL, Roche PM, 
            Lukyanov S, Zaraisky AG, Davidson MW, Chudakov DM
  TITLE     Far-red fluorescent tags for protein imaging in living tissues.
  JOURNAL   Biochem J. 2009 Mar 15;418(3):567-74. doi: 10.1042/BJ20081949.
  PUBMED    19143658
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Biochem J"; date: "2009-03-15- 15"; volume: "418"; issue: "3"; 
            pages: "567-74"
COMMENT     Sequence Label: tdKatushka2-Fibrillarin-7
FEATURES             Location/Qualifiers
     source          1..2547
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..739
                     /codon_start=1
                     /product="improved version of the oligomeric far-red 
                     fluorescent protein TurboFP635/Katushka (Luker et al., 
                     2015)"
                     /label=Katushka2S
                     /note="mammalian codon-optimized"
                     /translation="MVGEDSVLITENMHMKLYMEGTVNDHHFKCTSEGEGKPYEGTQTM
                     KIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERITTYEDGG
                     VLTATQDTSLQNGCLIYNVKINGVNFPSNGPVMQKKTLGWEASTEMLYPADSGLRGHAQ
                     MALKLVGGGYLHCSLKTTYRSKKPAKNLKMPGFYFVDRRLERIKEADKETYVEQHEMAV
                     ARYCDLPSKLGHS"
     CDS             35..739
                     /codon_start=1
                     /product="far-red-shifted derivative of red fluorescent 
                     protein from Entacmaea quadricolor, also known as Katushka 
                     (Shcherbo et al., 2007)"
                     /label=TurboFP635
                     /note="mammalian codon-optimized"
                     /translation="MVGEDSVLITENMHMKLYMEGTVNDHHFKCTSEGEGKPYEGTQTM
                     KIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERITTYEDGG
                     VLTATQDTSLQNGCLIYNVKINGVNFPSNGPVMQKKTLGWEASTEMLYPADSGLRGHAQ
                     MALKLVGGGYLHCSLKTTYRSKKPAKNLKMPGFYFVDRRLERIKEADKETYVEQHEMAV
                     ARYCDLPSKLGHS"
     CDS             809..1513
                     /codon_start=1
                     /product="improved version of the oligomeric far-red 
                     fluorescent protein TurboFP635/Katushka (Luker et al., 
                     2015)"
                     /label=Katushka2S
                     /note="mammalian codon-optimized"
                     /translation="MVGEDSVLITENMHMKLYMEGTVNDHHFKCTSEGEGKPYEGTQTM
                     KIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERITTYEDGG
                     VLTATQDTSLQNGCLIYNVKINGVNFPSNGPVMQKKTLGWEASTEMLYPADSGLRGHAQ
                     MALKLVGGGYLHCSLKTTYRSKKPAKNLKMPGFYFVDRRLERIKEADKETYVEQHEMAV
                     ARYCDLPSKLGHS"
     CDS             809..1513
                     /codon_start=1
                     /product="far-red-shifted derivative of red fluorescent 
                     protein from Entacmaea quadricolor, also known as Katushka 
                     (Shcherbo et al., 2007)"
                     /label=TurboFP635
                     /note="mammalian codon-optimized"
                     /translation="MVGEDSVLITENMHMKLYMEGTVNDHHFKCTSEGEGKPYEGTQTM
                     KIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERITTYEDGG
                     VLTATQDTSLQNGCLIYNVKINGVNFPSNGPVMQKKTLGWEASTEMLYPADSGLRGHAQ
                     MALKLVGGGYLHCSLKTTYRSKKPAKNLKMPGFYFVDRRLERIKEADKETYVEQHEMAV
                     ARYCDLPSKLGHS"
     CDS             complement(2309..2320)
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing 
                     et al., 2004)"
                     /label=TEE
                     /translation="NHKV"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg ggtgaggata gcgtgctgat
       61 caccgagaac atgcacatga aactgtacat ggagggcacc gtgaacgacc accacttcaa
      121 gtgcacatcc gagggcgaag gcaagcccta cgagggcacc cagaccatga agatcaaggt
      181 ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg gctaccagct tcatgtacgg
      241 cagcaaaacc tttatcaacc acacccaggg catccccgac ttctttaagc agtccttccc
      301 tgagggcttc acatgggaga ggatcaccac atacgaagac gggggcgtgc tgaccgctac
      361 ccaggacacc agcctccaga acggctgcct catctacaac gtcaagatca acggggtgaa
      421 cttcccatcc aacggccctg tgatgcagaa gaaaacactc ggctgggagg ccagcaccga
      481 gatgctgtac cccgctgaca gcggcctgag aggccatgcc cagatggccc tgaagctcgt
      541 gggcgggggc tacctgcact gctccctcaa gaccacatac agatccaaga aacccgctaa
      601 gaacctcaag atgcccggct tctacttcgt ggacaggaga ctggaaagaa tcaaggaggc
      661 cgacaaagag acctacgtcg agcagcacga gatggctgtg gccaggtact gcgacctgcc
      721 tagcaaactg gggcacagcg ggcatggcac cggcagcacc ggcagcggca gctccggtac
      781 cgcctcctcc gaggacaaca acatggccat ggtgggtgag gatagcgtgc tgatcaccga
      841 gaacatgcac atgaaactgt acatggaggg caccgtgaac gaccaccact tcaagtgcac
      901 atccgagggc gaaggcaagc cctacgaggg cacccagacc atgaagatca aggtggtcga
      961 gggcggccct ctccccttcg ccttcgacat cctggctacc agcttcatgt acggcagcaa
     1021 aacctttatc aaccacaccc agggcatccc cgacttcttt aagcagtcct tccctgaggg
     1081 cttcacatgg gagaggatca ccacatacga agacgggggc gtgctgaccg ctacccagga
     1141 caccagcctc cagaacggct gcctcatcta caacgtcaag atcaacgggg tgaacttccc
     1201 atccaacggc cctgtgatgc agaagaaaac actcggctgg gaggccagca ccgagatgct
     1261 gtaccccgct gacagcggcc tgagaggcca tgcccagatg gccctgaagc tcgtgggcgg
     1321 gggctacctg cactgctccc tcaagaccac atacagatcc aagaaacccg ctaagaacct
     1381 caagatgccc ggcttctact tcgtggacag gagactggaa agaatcaagg aggccgacaa
     1441 agagacctac gtcgagcagc acgagatggc tgtggccagg tactgcgacc tgcctagcaa
     1501 actggggcac agctccggac tcagatctcg agccatgaag ccaggattca gtccccgtgg
     1561 gggtggcttt ggcggccgag ggggctttgg tgaccgtggt ggtcgtggag gccgaggggg
     1621 ctttggcggg ggccgaggcc aaggcggagg ctttagaggt cgtggacgag gaggaggtgg
     1681 aggcggcggc ggcggtggag gaggaggaag aggtggtgga ggcttccatt ttggtggcaa
     1741 ccggggtcgt ggtcggggag gaaaaagagg aaaccagtcg gggaagaatg tgatggtgga
     1801 gccgcatcgg catgagggtg tcttcatttg tcggggaaag gaagatgcac tggtcaccaa
     1861 gaacctggtc cctggggaat cagtttatgg agagaagaga gtctcgattt cggaaggaga
     1921 tgacaaaatt gagtaccgag cctggaaccc cttccgctcc aagcttgcag cagcaatcct
     1981 gggtggtgtg gaccagatcc acatcaaacc gggggctaag gttctctacc tcggggctgc
     2041 ctcgggcacc acggtctccc atgtctctga catcgttggt ccggatggtc tagtctatgc
     2101 agtcgagttc tcccaccgct ctggccgtga cctcattaac ttggccaaga agaggaccaa
     2161 catcattcct gtgatcgagg atgctcgaca cccacacaaa taccgcatgc tcatcgcaat
     2221 ggtggatgtg atctttgctg atgtggccca gccagaccag acccggattg tggccctgaa
     2281 tgcccacacc ttcctgcgta atggaggaca ctttgtgatt tccattaagg ccaactgcat
     2341 tgactccaca gcctcagccg aggccgtgtt tgcctccgaa gtgaaaaaga tgcaacagga
     2401 gaacatgaag ccgcaggagc agttgaccct tgagccatat gaaagagacc atgccgtggt
     2461 cgtgggagtg tacaggccac cccccaaggt gaagaactga ggatccaccg gatctagata
     2521 actgatcata atcagccata ccacatg
//
