LOCUS       sequence-86961-e        1755 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Golgi/ER Transport, Excitation: 586, Emission: 632.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Shcherbo D, Murphy CS, Ermakova GV, Solovieva EA, Chepurnykh TV, 
            Shcheglov AS, Verkhusha VV, Pletnev VZ, Hazelwood KL, Roche PM, 
            Lukyanov S, Zaraisky AG, Davidson MW, Chudakov DM
  TITLE     Far-red fluorescent tags for protein imaging in living tissues.
  JOURNAL   Biochem J. 2009 Mar 15;418(3):567-74. doi: 10.1042/BJ20081949.
  PUBMED    19143658
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Biochem J"; date: "2009-03-15- 15"; volume: "418"; issue: "3"; 
            pages: "567-74"
COMMENT     Sequence Label: tdKatushka2-VSVG-16
FEATURES             Location/Qualifiers
     source          1..1755
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      214..223
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             220..924
                     /codon_start=1
                     /product="improved version of the oligomeric far-red 
                     fluorescent protein TurboFP635/Katushka (Luker et al., 
                     2015)"
                     /label=Katushka2S
                     /note="mammalian codon-optimized"
                     /translation="MVGEDSVLITENMHMKLYMEGTVNDHHFKCTSEGEGKPYEGTQTM
                     KIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERITTYEDGG
                     VLTATQDTSLQNGCLIYNVKINGVNFPSNGPVMQKKTLGWEASTEMLYPADSGLRGHAQ
                     MALKLVGGGYLHCSLKTTYRSKKPAKNLKMPGFYFVDRRLERIKEADKETYVEQHEMAV
                     ARYCDLPSKLGHS"
     CDS             220..924
                     /codon_start=1
                     /product="far-red-shifted derivative of red fluorescent 
                     protein from Entacmaea quadricolor, also known as Katushka 
                     (Shcherbo et al., 2007)"
                     /label=TurboFP635
                     /note="mammalian codon-optimized"
                     /translation="MVGEDSVLITENMHMKLYMEGTVNDHHFKCTSEGEGKPYEGTQTM
                     KIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERITTYEDGG
                     VLTATQDTSLQNGCLIYNVKINGVNFPSNGPVMQKKTLGWEASTEMLYPADSGLRGHAQ
                     MALKLVGGGYLHCSLKTTYRSKKPAKNLKMPGFYFVDRRLERIKEADKETYVEQHEMAV
                     ARYCDLPSKLGHS"
     CDS             994..1698
                     /codon_start=1
                     /product="improved version of the oligomeric far-red 
                     fluorescent protein TurboFP635/Katushka (Luker et al., 
                     2015)"
                     /label=Katushka2S
                     /note="mammalian codon-optimized"
                     /translation="MVGEDSVLITENMHMKLYMEGTVNDHHFKCTSEGEGKPYEGTQTM
                     KIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERITTYEDGG
                     VLTATQDTSLQNGCLIYNVKINGVNFPSNGPVMQKKTLGWEASTEMLYPADSGLRGHAQ
                     MALKLVGGGYLHCSLKTTYRSKKPAKNLKMPGFYFVDRRLERIKEADKETYVEQHEMAV
                     ARYCDLPSKLGHS"
     CDS             994..1698
                     /codon_start=1
                     /product="far-red-shifted derivative of red fluorescent 
                     protein from Entacmaea quadricolor, also known as Katushka 
                     (Shcherbo et al., 2007)"
                     /label=TurboFP635
                     /note="mammalian codon-optimized"
                     /translation="MVGEDSVLITENMHMKLYMEGTVNDHHFKCTSEGEGKPYEGTQTM
                     KIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERITTYEDGG
                     VLTATQDTSLQNGCLIYNVKINGVNFPSNGPVMQKKTLGWEASTEMLYPADSGLRGHAQ
                     MALKLVGGGYLHCSLKTTYRSKKPAKNLKMPGFYFVDRRLERIKEADKETYVEQHEMAV
                     ARYCDLPSKLGHS"
ORIGIN
        1 ggttggttca gtagttggaa gagctctatt gcctcttttt tctttatcat agggttaatc
       61 attggactat tcttggttct ccgagttggt atttatcttt gcattaaatt aaagcacacc
      121 aagaaaagac agatttatac agacatagag atgaaccgac ttggaaacag aattctgcag
      181 tcgacggtac cgcgggcccg ggatccaccg gtcgccacca tggtgggtga ggatagcgtg
      241 ctgatcaccg agaacatgca catgaaactg tacatggagg gcaccgtgaa cgaccaccac
      301 ttcaagtgca catccgaggg cgaaggcaag ccctacgagg gcacccagac catgaagatc
      361 aaggtggtcg agggcggccc tctccccttc gccttcgaca tcctggctac cagcttcatg
      421 tacggcagca aaacctttat caaccacacc cagggcatcc ccgacttctt taagcagtcc
      481 ttccctgagg gcttcacatg ggagaggatc accacatacg aagacggggg cgtgctgacc
      541 gctacccagg acaccagcct ccagaacggc tgcctcatct acaacgtcaa gatcaacggg
      601 gtgaacttcc catccaacgg ccctgtgatg cagaagaaaa cactcggctg ggaggccagc
      661 accgagatgc tgtaccccgc tgacagcggc ctgagaggcc atgcccagat ggccctgaag
      721 ctcgtgggcg ggggctacct gcactgctcc ctcaagacca catacagatc caagaaaccc
      781 gctaagaacc tcaagatgcc cggcttctac ttcgtggaca ggagactgga aagaatcaag
      841 gaggccgaca aagagaccta cgtcgagcag cacgagatgg ctgtggccag gtactgcgac
      901 ctgcctagca aactggggca cagcgggcat ggcaccggca gcaccggcag cggcagctcc
      961 ggtaccgcct cctccgagga caacaacatg gccatggtgg gtgaggatag cgtgctgatc
     1021 accgagaaca tgcacatgaa actgtacatg gagggcaccg tgaacgacca ccacttcaag
     1081 tgcacatccg agggcgaagg caagccctac gagggcaccc agaccatgaa gatcaaggtg
     1141 gtcgagggcg gccctctccc cttcgccttc gacatcctgg ctaccagctt catgtacggc
     1201 agcaaaacct ttatcaacca cacccagggc atccccgact tctttaagca gtccttccct
     1261 gagggcttca catgggagag gatcaccaca tacgaagacg ggggcgtgct gaccgctacc
     1321 caggacacca gcctccagaa cggctgcctc atctacaacg tcaagatcaa cggggtgaac
     1381 ttcccatcca acggccctgt gatgcagaag aaaacactcg gctgggaggc cagcaccgag
     1441 atgctgtacc ccgctgacag cggcctgaga ggccatgccc agatggccct gaagctcgtg
     1501 ggcgggggct acctgcactg ctccctcaag accacataca gatccaagaa acccgctaag
     1561 aacctcaaga tgcccggctt ctacttcgtg gacaggagac tggaaagaat caaggaggcc
     1621 gacaaagaga cctacgtcga gcagcacgag atggctgtgg ccaggtactg cgacctgcct
     1681 agcaaactgg ggcacagctc cggactcaga tcttgagcgg ccgcgactct agatcataat
     1741 cagccatacc acatt
//
