LOCUS       sequence-86965-e         993 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 588, Emission: 635.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 993)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKate-CytERM-N-17
FEATURES             Location/Qualifiers
     source          1..993
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      223..232
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             229..942
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
ORIGIN
        1 gccatgctag tgacgtcaga tccgctagcg ctaccggact cagatctggt accccttgca
       61 gcttccagtt gtcaggaaga gaaggctccc atggatcctg tggtggtgct ggggctctgt
      121 ctctcctgtt tgcttctcct ttcactctgg aaacagagct atgggggagg gaagcttcga
      181 attctgcagt cgacggtacc gcgggcccgg gatccaccgg tcgccaccat ggtgtctaag
      241 ggcgaagagc tgattaagga gaacatgcac atgaagctgt acatggaggg caccgtgaac
      301 aaccaccact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc
      361 atgagaatca aggtggtcga gggcggccct ctccccttcg ccttcgacat cctggctacc
      421 agcttcatgt acggcagcaa aaccttcatc aaccacaccc agggcatccc cgacttcttt
      481 aagcagtcct tccctgaggg cttcacatgg gagagagtca ccacatacga agacgggggc
      541 gtgctgaccg ctacccagga caccagcctc caggacggct gcctcatcta caacgtcaag
      601 atcagagggg tgaacttccc atccaacggc cctgtgatgc agaagaaaac actcggctgg
      661 gaggcctcca ccgagatgct gtaccccgct gacggcggcc tggaaggcag aagcgacatg
      721 gccctgaagc tcgtgggcgg gggccacctg atctgcaact tgaagaccac atacagatcc
      781 aagaaacccg ctaagaacct caagatgccc ggcgtctact atgtggacag aagactggaa
      841 agaatcaagg aggccgacaa agagacctac gtcgagcagc acgaggtggc tgtggccaga
      901 tactgcgacc tccctagcaa actggggcac aaacttaatt gagcggccgc gactctagat
      961 cataatcagc cataccacat tgtagggtag ccc
//
