LOCUS       sequence-86966-e        1388 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 588, Emission: 635.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1388)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 1388)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKate-H1-10
FEATURES             Location/Qualifiers
     source          1..1388
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      630..639
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             636..1349
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
ORIGIN
        1 cgtcagatcc gctagccgcc accatgaccg agaactccac ctccgccccg gcggcgaagc
       61 ccaaacgggc caaggcttcc aagaagtcca cggaccaccc caagtattca gacatgatcg
      121 tggctgctat ccaggcagag aagaaccgtg ccggctcctc gcgccagtcc atccaaaagt
      181 atatcaagag ccactacaag gtgggtgaga acgccgactc ccagatcaag ttgtccatca
      241 agcgcctagt gaccaccggt gttctcaagc aaaccaaagg ggtgggcgcc tcggggtcct
      301 tcaggctggc caagggcgat gagcccaaaa ggtcggtggc tttcaagaag accaagaagg
      361 aagtcaagaa agtggccact ccaaagaagg cagccaagcc caagaaggct gcctccaaag
      421 ccccaagcaa gaaacccaaa gccacccctg tcaagaaggc caagaagaag ccggctgcca
      481 cgcccaagaa agccaaaaag cccaaggttg tcaaagtcaa accagtcaag gcctccaaac
      541 ccaagaaggc caaaaccgtg aagcccaaag ccaagtcgag tgccaagagg gggagcaaga
      601 agaagtcggg aagcacggat ccaccggtcg ccaccatggt gtctaagggc gaagagctga
      661 ttaaggagaa catgcacatg aagctgtaca tggagggcac cgtgaacaac caccacttca
      721 agtgcacatc cgagggcgaa ggcaagccct acgagggcac ccagaccatg agaatcaagg
      781 tggtcgaggg cggccctctc cccttcgcct tcgacatcct ggctaccagc ttcatgtacg
      841 gcagcaaaac cttcatcaac cacacccagg gcatccccga cttctttaag cagtccttcc
      901 ctgagggctt cacatgggag agagtcacca catacgaaga cgggggcgtg ctgaccgcta
      961 cccaggacac cagcctccag gacggctgcc tcatctacaa cgtcaagatc agaggggtga
     1021 acttcccatc caacggccct gtgatgcaga agaaaacact cggctgggag gcctccaccg
     1081 agatgctgta ccccgctgac ggcggcctgg aaggcagaag cgacatggcc ctgaagctcg
     1141 tgggcggggg ccacctgatc tgcaacttga agaccacata cagatccaag aaacccgcta
     1201 agaacctcaa gatgcccggc gtctactatg tggacagaag actggaaaga atcaaggagg
     1261 ccgacaaaga gacctacgtc gagcagcacg aggtggctgt ggccagatac tgcgacctcc
     1321 ctagcaaact ggggcacaaa cttaattgag cggccgcgac tctagatcat aatcagccat
     1381 accacatt
//
