LOCUS       sequence-86967-e        1195 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 588, Emission: 635.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1195)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 1195)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKate-H2A-10
FEATURES             Location/Qualifiers
     source          1..1195
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      437..446
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             443..1156
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
ORIGIN
        1 gtcagatccg ctagccgcca ccatgtcggg acgtggcaag cagggcggca aagctcgcgc
       61 caaggccaag acccgctctt ctcgggccgg gcttcagttt cccgtaggcc gagtgcatcg
      121 cctgctccgc aaaggcaact atgcggagcg ggtcggtgct ggagcgccgg tgtacctggc
      181 ggcggtgctg gagtacctga ccgccgagat cctggagctg gctggcaacg cggcccgcga
      241 caacaagaag actcgcatca tcccgcgtca cctccagctg gccatccgca acgatgagga
      301 gctcaacaag cttctgggca aagtcaccat cgcacagggt ggcgtcctgc ccaacatcca
      361 ggccgtgcta ctgcccaaga agaccgagag ccaccacaag gcgaagggca agtcgggaag
      421 cacggatcca ccggtcgcca ccatggtgtc taagggcgaa gagctgatta aggagaacat
      481 gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga
      541 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggtgg tcgagggcgg
      601 ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca gcaaaacctt
      661 catcaaccac acccagggca tccccgactt ctttaagcag tccttccctg agggcttcac
      721 atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag
      781 cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact tcccatccaa
      841 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc tccaccgaga tgctgtaccc
      901 cgctgacggc ggcctggaag gcagaagcga catggccctg aagctcgtgg gcgggggcca
      961 cctgatctgc aacttgaaga ccacatacag atccaagaaa cccgctaaga acctcaagat
     1021 gcccggcgtc tactatgtgg acagaagact ggaaagaatc aaggaggccg acaaagagac
     1081 ctacgtcgag cagcacgagg tggctgtggc cagatactgc gacctcccta gcaaactggg
     1141 gcacaaactt aattgagcgg ccgcgactct agatcataat cagccatacc acatt
//
