LOCUS       sequence-86969-e        1257 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 588, Emission: 635.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1257)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 1257)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKate-H3-23
FEATURES             Location/Qualifiers
     source          1..1257
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      22..31
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    439..491
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      499..508
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             505..1218
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
ORIGIN
        1 gtgacgtcag atccgctagc cgccaccatg gctcgtacta aacagacagc tcggaaatcc
       61 accggcggta aagcgccacg caagcagctg gctaccaagg ctgctcgcaa gagcgcgccg
      121 gctaccggcg gcgtgaaaaa gcctcaccgt taccgcccgg gcactgtggc tctgcgcgag
      181 atccgccgct accaaaagtc gaccgagttg ctgattcgga agctgccgtt ccagcgcctg
      241 gtgcgagaaa tcgcccaaga cttcaagacc gatcttcgct tccagagctc tgcggtgatg
      301 gcgctgcagg aggcttgtga ggcctacttg gtagggctct ttgaggacac aaacctttgc
      361 gccatccatg ctaagcgagt gactattatg cccaaagaca tccagctcgc tcgccgcatt
      421 cgcggagaaa gagcggatct cgagctcaag cttcgaattc tgcagtcgac ggtaccgcgg
      481 gcccgggatc caccggtcgc caccatggtg tctaagggcg aagagctgat taaggagaac
      541 atgcacatga agctgtacat ggagggcacc gtgaacaacc accacttcaa gtgcacatcc
      601 gagggcgaag gcaagcccta cgagggcacc cagaccatga gaatcaaggt ggtcgagggc
      661 ggccctctcc ccttcgcctt cgacatcctg gctaccagct tcatgtacgg cagcaaaacc
      721 ttcatcaacc acacccaggg catccccgac ttctttaagc agtccttccc tgagggcttc
      781 acatgggaga gagtcaccac atacgaagac gggggcgtgc tgaccgctac ccaggacacc
      841 agcctccagg acggctgcct catctacaac gtcaagatca gaggggtgaa cttcccatcc
      901 aacggccctg tgatgcagaa gaaaacactc ggctgggagg cctccaccga gatgctgtac
      961 cccgctgacg gcggcctgga aggcagaagc gacatggccc tgaagctcgt gggcgggggc
     1021 cacctgatct gcaacttgaa gaccacatac agatccaaga aacccgctaa gaacctcaag
     1081 atgcccggcg tctactatgt ggacagaaga ctggaaagaa tcaaggaggc cgacaaagag
     1141 acctacgtcg agcagcacga ggtggctgtg gccagatact gcgacctccc tagcaaactg
     1201 gggcacaaac ttaattgagc ggccgcgact ctagatcata atcagccata ccacatg
//
