LOCUS       sequence-8697-ex        6153 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6153)
  AUTHORS   Budhu A, Chen Y, Kim JW, Forgues M, Valerie K, Harris CC, Wang XW
  TITLE     Induction of a unique gene expression profile in primary human 
            hepatocytes by hepatitis C virus core, NS3 and NS5A proteins.
  JOURNAL   Carcinogenesis. 2007 Jul . 28(7):1552-60.
  PUBMED    17404395
REFERENCE   2  (bases 1 to 6153)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Carcinogenesis. 2007 Jul . 28(7):1552-60."
COMMENT     Sequence Label: pCMV-Tag1-NS3
FEATURES             Location/Qualifiers
     source          1..6153
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        67..370
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        371..574
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     525..545
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        620..638
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             699..722
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(2654..2673)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2655..2673)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    2947..3068
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2984..3003)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3038..3057
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3075..3530)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3212..3233)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3424..3443
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3557..3661
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3663..4020
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3663..3854
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3686..3706)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3871..4006
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4055..4849
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4055..4849
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4109..4128)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4719..4738
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5037..5056)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5081..5128
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5457..6045
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5946..5965
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
       61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      541 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      601 gcgattacgc caagctcgaa attaaccctc actaaaggga acaaaagctg gagctccacc
      661 gcggtggcgg ccgctctagc ccgggcggat ccaccatgga ttacaaggat gacgacgata
      721 agatcttggc gcccatcacg gcctactccc aacaaacgcg gggcgtactt ggttgcatca
      781 tcactagcct cacaggccgg gacaagaacc aggtcgaagg ggaggttcaa gtggtttcta
      841 ccgcaacaca atctttcctg gcgacctgca tcaacggcgt gtgctggact gtctaccatg
      901 gcgctggctc gaagacccta gccggtccaa aaggtccaat cacccaaatg tacaccaatg
      961 tagacctgga cctcgtcggc tggcaggcgc cccccggggc gcgctccatg acaccatgca
     1021 gctgtggcag ctcggacctt tacttggtca cgagacatgc tgatgtcatt ccggtgcgcc
     1081 ggcgaggcga cagcagggga agtctactct cccccaggcc cgtctcctac ctgaaaggct
     1141 cctcgggtgg tccattgctt tgcccttcgg ggcacgtcgt gggcgtcttc cgggctgctg
     1201 tgtgcacccg gggggtcgcg aaggcggtgg acttcatacc cgttgagtct atggaaacta
     1261 ccatgcggtc tccggtcttc acagacaact caaccccccc ggctgtaccg cagacattcc
     1321 aagtggcaca tctgcacgct cctactggca gcggcaagag caccaaagtg ccggctgcgt
     1381 atgcagccca agggtacaag gtgctcgtcc tgaacccgtc cgttgccgcc accttagggt
     1441 ttggggcgta tatgtccaag gcacacggta tcgaccctaa catcagaact ggggtaagga
     1501 ccattaccac gggcggctcc attacgtact ccacctatgg caagttcctt gccgacggtg
     1561 gctgttctgg gggcgcctat gacatcataa tatgtgatga gtgccactca actgactcga
     1621 ctaccatctt gggcatcggc acagtcctgg accaagcgga gacggctgga gcgcggctcg
     1681 tcgtgctcgc caccgctaca cctccgggat cggttaccgt gccacacccc aatatcgagg
     1741 aaataggcct gtccaacaat ggagagatcc ccttctatgg caaagccatc cccattgagg
     1801 ccatcaaggg ggggaggcat ctcattttct gccattccaa gaagaaatgt gacgagctcg
     1861 ccgcaaagct gacaggcctc ggactgaacg ctgtagcata ttaccggggc cttgatgtgt
     1921 ccgtcatacc gcctatcgga gacgtcgttg tcgtggcaac agacgctcta atgacgggtt
     1981 tcaccggcga ttttgactca gtgatcgact gcaatacatg tgtcacccag acagtcgact
     2041 tcagcttgga tcccaccttc accattgaga cgacgaccgt gccccaagac gcggtgtcgc
     2101 gctcgcaacg gcgaggtaga actggcaggg gtaggagtgg catctacagg tttgtgactc
     2161 caggagaacg gccctcgggc atgttcgatt cttcggtcct gtgtgagtgc tatgacgcgg
     2221 gctgtgcttg gtatgagctc acgcccgctg agacctcggt taggttgcgg gcttacctaa
     2281 atacaccagg gttgcccgtc tgccaggacc atctggagtt ctgggagagc gtcttcacag
     2341 gcctcaccca catagatgcc cacttcctgt cccagactaa acaggcagga gacaactttc
     2401 cttacctggt ggcatatcaa gctacagtgt gcgccagggc tcaagctcca cctccatcgt
     2461 gggaccaaat gtggaagtgt ctcatacggc tgaaacctac actgcacggg ccaacacccc
     2521 tgctgtatag gctaggagcc gtccaaaatg aggtcatcct cacacacccc ataactaaat
     2581 acatcatggc atgcatgtcg gctgacctgg aggtcgtcac ttagtagggt accaggtaag
     2641 tgtacccaat tcgccctata gtgagtcgta ttacaattca ctcgatcgcc cttcccaaca
     2701 gttgcgcagc ctgaatggcg aatggagatc caatttttaa gtgtataatg tgttaaacta
     2761 ctgattctaa ttgtttgtgt attttagatt cacagtccca aggctcattt caggcccctc
     2821 agtcctcaca gtctgttcat gatcataatc agccatacca catttgtaga ggttttactt
     2881 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     2941 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     3001 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     3061 gtatcttaac gcgtaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
     3121 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
     3181 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
     3241 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
     3301 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
     3361 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
     3421 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
     3481 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac
     3541 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     3601 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagaa
     3661 tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     3721 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     3781 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     3841 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     3901 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     3961 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     4021 gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc
     4081 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
     4141 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
     4201 caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg
     4261 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
     4321 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
     4381 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
     4441 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
     4501 agccggtctt gtcgatcagg atgatctgga cgaagaacat caggggctcg cgccagccga
     4561 actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg
     4621 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
     4681 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
     4741 tgaagaactt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
     4801 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg
     4861 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc
     4921 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     4981 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ctagggggag gctaactgaa
     5041 acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat
     5101 aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac
     5161 tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc
     5221 ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca
     5281 ggccctgcca tagcctcagg ttactcatat atactttaga ttgatttaaa acttcatttt
     5341 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     5401 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     5461 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     5521 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     5581 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     5641 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     5701 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     5761 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     5821 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     5881 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     5941 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     6001 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     6061 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     6121 tcccctgatt ctgtggataa ccgtattacc gcc
//
