LOCUS       sequence-86970-e        1158 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 488, Emission: 635.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1158)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 1158)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKate-H4-23
FEATURES             Location/Qualifiers
     source          1..1158
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    337..389
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      397..406
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             403..1116
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
ORIGIN
        1 acgtcagatc cgctagccgc caccatgtct ggccgcggca aaggcgggaa gggtcttggc
       61 aaaggcggcg ctaagcgcca ccgtaaagta ctgcgcgaca atatccaggg catcaccaag
      121 ccggccatcc ggcgccttgc tcgccgcggc ggcgtgaagc gcatctccgg cctcatctac
      181 gaggagactc gcggggtgct gaaggtgttc ctggagaacg tgatccggga cgccgtgacc
      241 tatacagagc acgccaagcg caagacggtc accgccatgg atgtggtcta cgcgctcaag
      301 cgccagggcc gcaccctcta cggtttcggt ggggatctcg agctcaagct tcgaattctg
      361 cagtcgacgg taccgcgggc ccgggatcca ccggtcgcca ccatggtgtc taagggcgaa
      421 gagctgatta aggagaacat gcacatgaag ctgtacatgg agggcaccgt gaacaaccac
      481 cacttcaagt gcacatccga gggcgaaggc aagccctacg agggcaccca gaccatgaga
      541 atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg acatcctggc taccagcttc
      601 atgtacggca gcaaaacctt catcaaccac acccagggca tccccgactt ctttaagcag
      661 tccttccctg agggcttcac atgggagaga gtcaccacat acgaagacgg gggcgtgctg
      721 accgctaccc aggacaccag cctccaggac ggctgcctca tctacaacgt caagatcaga
      781 ggggtgaact tcccatccaa cggccctgtg atgcagaaga aaacactcgg ctgggaggcc
      841 tccaccgaga tgctgtaccc cgctgacggc ggcctggaag gcagaagcga catggccctg
      901 aagctcgtgg gcgggggcca cctgatctgc aacttgaaga ccacatacag atccaagaaa
      961 cccgctaaga acctcaagat gcccggcgtc tactatgtgg acagaagact ggaaagaatc
     1021 aaggaggccg acaaagagac ctacgtcgag cagcacgagg tggctgtggc cagatactgc
     1081 gacctcccta gcaaactggg gcacaaactt aattgagcgg ccgcgactct agatcataat
     1141 cagccatacc acattgag
//
