LOCUS       sequence-86988-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 588, Emission: 635.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Shcherbo D, Murphy CS, Ermakova GV, Solovieva EA, Chepurnykh TV, 
            Shcheglov AS, Verkhusha VV, Pletnev VZ, Hazelwood KL, Roche PM, 
            Lukyanov S, Zaraisky AG, Davidson MW, Chudakov DM
  TITLE     Far-red fluorescent tags for protein imaging in living tissues.
  JOURNAL   Biochem J. 2009 Mar 15;418(3):567-74. doi: 10.1042/BJ20081949.
  PUBMED    19143658
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Biochem J"; date: "2009-03-15- 15"; volume: "418"; issue: "3"; 
            pages: "567-74"
COMMENT     Sequence Label: mKate1.31-CytERM-N-17
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      71..80
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      209..218
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             215..913
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
ORIGIN
        1 acagagctat gggggaggga agcttcgaat tctgcagtcg acggtaccgc gggcccggga
       61 tccaccggtc gccaccatgg atcctgtggt ggtgctgggg ctctgtctct cctgtttgct
      121 tctcctttca ctctggaaac agagctatgg gggagggaag cttcgaattc tgcagtcgac
      181 ggtaccgcgg gcccgggatc caccggtcgc caccatggtg agcgagctga ttaaggagaa
      241 catgcacatg aagctgtaca tggagggcac cgtgaacaac caccacttca agtgcacatc
      301 cgagggcgaa ggcaagccct acgagggcac ccagaccatg agaatcaagg cggtcgaggg
      361 cggccctctc cccttcgcct tcgacatcct ggctaccagc ttcatgtacg gcagcaaaac
      421 cttcatcaac cacacccagg gcatccccga cttctttaag cagtccttcc ccgagggctt
      481 cacatgggag agagtcacca catacgaaga cgggggcgtg ctgaccgcta cccaggacac
      541 cagcctccag gacggctgcc tcatctacaa cgtcaagatc agaggggtga acttcccatc
      601 caacggccct gtgatgcaga agaaaacact cggctgggag gcctccaccg agaccctgta
      661 ccccgctgac ggcggcctgg aaggcagagc cgacatggcc ctgaagctcg tgggcggggg
      721 ccacctgatc tgcaacttga agaccacata cagatccaag aaacccgcta agaacctcaa
      781 gatgcccggc gtctactatg tggacagaag actggaaaga atcaaggagg ccgacaaaga
      841 gacctacgtc gagcagcacg aggtggctgt ggccagatac tgcgacctcc ctagcaaact
      901 ggggcacaga tgagcggccg cgactctaga tcataatcag ccataccaca tttgtagagg
      961 ttttacttgc tttaaaaaac ctcccacacc tccc
//
