LOCUS       sequence-8699-ex        3324 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Wang W, Budhu A, Forgues M, Wang XW
  TITLE     Temporal and spatial control of nucleophosmin by the Ran-Crm1 
            complex in centrosome duplication.
  JOURNAL   Nat Cell Biol. 2005 Aug . 7(8):823-30.
  PUBMED    16041368
REFERENCE   2  (bases 1 to 3324)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2005 Aug . 7(8):823-30."
COMMENT     Sequence Label: Flag-hCRM1
FEATURES             Location/Qualifiers
     source          1..3324
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..69
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 atggactaca aagaccatga cggtgattat aaagatcatg acatcgatta caaggatgac
       61 gatgacaagc ttgcggccgc gaattcatcg atagatctga tatcggtacc accagcaatt
      121 atgacaatgt tagcagacca tgcagctcgt cagctgcttg atttcagcca aaaactggat
      181 atcaacttat tagataatgt ggtgaattgc ttataccatg gagaaggagc ccagcaaaga
      241 atggctcaag aagtactgac acatttaaag gagcatcctg atgcttggac aagagtcgac
      301 acaattttgg aattttctca gaatatgaat acgaaatact atggactaca aattttggaa
      361 aatgtgataa aaacaaggtg gaagattctt ccaaggaacc agtgcgaagg aataaaaaaa
      421 tacgttgttg gcctcattat caagacgtca tctgacccaa cttgtgtaga gaaagaaaag
      481 gtgtatatcg gaaaattaaa tatgatcctt gttcagatac tgaaacaaga atggcccaaa
      541 cattggccaa cttttatcag tgatattgtt ggagcaagta ggaccagcga aagtctctgt
      601 caaaataata tggtgattct taaactcttg agtgaagaag tatttgattt ctctagtgga
      661 cagataaccc aagtcaaatc taagcattta aaagacagca tgtgcaatga attctcacag
      721 atatttcaac tgtgtcagtt tgtaatggaa aattctcaaa atgctccact tgtacatgca
      781 accttggaaa cattgctcag atttctgaac tggattcccc tgggatatat ttttgagacc
      841 aaattaatca gcacattgat ttataagttc ctgaatgttc caatgtttcg aaatgtctct
      901 ctgaagtgcc tcactgagat tgctggtgtg agtgtaagcc aatatgaaga acaatttgta
      961 acactattta ctctgacaat gatgcaacta aagcagatgc ttcctttaaa taccaatatt
     1021 cgacttgcgt actcaaatgg aaaagatgat gaacagaact tcattcaaaa tctcagtttg
     1081 tttctctgca cctttcttaa ggaacatgat caacttatag aaaaaagatt aaatctcagg
     1141 gaaactctta tggaggccct tcattatatg ttgttggtat ctgaagtaga agaaactgaa
     1201 atctttaaaa tttgtcttga atactggaat catttggctg ctgaactcta tagagagagt
     1261 ccattctcta catctgcctc tccgttgctt tctggaagtc aacattttga tgttcctccc
     1321 aggagacagc tatatttgcc catgttattc aaggtccgtt tattaatggt tagtcgaatg
     1381 gctaaaccag aggaagtatt ggttgtagag aatgatcaag gagaagttgt gagagaattc
     1441 atgaaggata cagattccat aaatttgtat aagaatatga gggaaacatt ggtttatctt
     1501 actcatctgg attatgtaga tacagaaaga ataatgacag agaagcttca caatcaagtg
     1561 aatggtacag agtggtcatg gaaaaatttg aatacattgt gttgggcaat aggctccatt
     1621 agtggagcaa tgcatgaaga ggacgaaaaa cgatttcttg ttactgttat aaaggatcta
     1681 ttaggattat gtgaacagaa aagaggcaaa gataataaag ctattattgc atcaaatatc
     1741 atgtacatag taggtcaata cccacgtttt ttgagagctc actggaaatt tctgaagact
     1801 gtagttaaca agctgttcga attcatgcat gagacccatg atggagtcca ggatatggct
     1861 tgtgatactt tcattaaaat agcccaaaaa tgccgcaggc atttcgttca ggttcaggtt
     1921 ggagaagtga tgccatttat tgatgaaatt ttgaacaaca ttaacactat tatttgtgat
     1981 cttcagcctc aacaggttca tacgttttat gaagctgtgg ggtacatgat tggtgcacaa
     2041 acagatcaaa cagtacaaga acacttgata gaaaagtaca tgttactccc taatcaagtg
     2101 tgggatagta taatccagca ggcaaccaaa aatgtggata tactgaaaga tcctgaaaca
     2161 gtcaagcagc ttggtagcat tttgaaaaca aatgtgagag cctgcaaagc tgttggacac
     2221 ccctttgtaa ttcagcttgg aagaatttat ttagatatgc ttaatgtata caagtgcctc
     2281 agtgaaaata tttctgcagc tatccaagct aatggtgaaa tggttacaaa gcaaccattg
     2341 attagaagta tgcgaactgt aaaaagggaa actttaaagt taatatctgg ttgggtgagc
     2401 cgatccaatg atccacagat ggtcgctgaa aattttgttc cccctctgtt ggatgcagtt
     2461 ctcattgatt atcagagaaa tgtcccagct gctagagaac cagaagtgct tagtactatg
     2521 gccataattg tcaacaagtt agggggacat ataacagctg aaatacctca aatatttgat
     2581 gctgtttttg aatgcacatt gaatatgata aataaggact ttgaagaata tcctgaacat
     2641 agaacgaact ttttcttact acttcaggct gtcaattctc attgtttccc agcattcctt
     2701 gctattccac ctacacagtt taaacttgtt ttggattcca tcatttgggc tttcaaacat
     2761 actatgagga atgtcgcaga tacgggctta cagatacttt ttacactctt acaaaatgtt
     2821 gcacaagaag aagctgcagc tcagagtttt tatcaaactt atttttgtga tattctccag
     2881 catatctttt ctgttgtgac agacacttca catactgctg gtttaacaat gcatgcatca
     2941 attcttgcat atatgtttaa tttggttgaa gaaggaaaaa taagtacatc attaaatcct
     3001 ggaaatccag ttaacaacca aatctttctt caggaatatg tggctaatct ccttaagtcg
     3061 gccttccctc acctacaaga tgctcaagta aagctctttg tgacagggct tttcagctta
     3121 aatcaagata ttcctgcttt caaggaacat ttaagagatt tcctagttca aataaaggaa
     3181 tttgcaggtg aagacacttc tgatttgttt ttggaagaga gagaaatagc cctacggcag
     3241 gctgatgaag agaaacataa acgtcaaatg tctgtccctg gcatctttaa tccacatgag
     3301 attccagaag aaatgtgtga ttaa
//
