LOCUS       sequence-87009-e        3552 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus, Excitation: 580, Emission: 608.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Shemiakina II, Ermakova GV, Cranfill PJ, Baird MA, Evans RA, 
            Souslova EA, Staroverov DB, Gorokhovatsky AY, Putintseva EV, 
            Gorodnicheva TV, Chepurnykh TV, Strukova L, Lukyanov S, Zaraisky AG,
            Davidson MW, Chudakov DM, Shcherbo D
  TITLE     A monomeric red fluorescent protein with low cytotoxicity.
  JOURNAL   Nat Commun. 2012;3:1204. doi: 10.1038/ncomms2208.
  PUBMED    23149748
REFERENCE   2  (bases 1 to 3552)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012"; volume: "3:1204. doi"; pages: " 
            10.1038/ncomms2208"
COMMENT     Sequence Label: FusionRed-CAF1-C-10
FEATURES             Location/Qualifiers
     source          1..3552
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..730
                     /codon_start=1
                     /product="monomeric red fluorescent protein optimized for 
                     use as a fusion tag (Shemiakina et al., 2012)"
                     /label=FusionRed
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMPMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFMYGSRTFIKHPPGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKVRGVNFPANGPVMQKKTLGWEASTETMYPADGGLEGACDMAL
                     KLVGGGHLICNLETTYRSKKPATNLKMPGVYNVDHRLERIKEADDETYVEQHEVAVARY
                     STGGAGDGGK"
     protein_bind    2561..2572
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
ORIGIN
        1 accgtcagat ccgctagcgc taccggtcgc caccatggtg agcgagctga ttaaggagaa
       61 catgcccatg aagctgtaca tggagggcac cgtgaacaac caccacttca agtgcacatc
      121 cgagggcgaa ggcaagccct acgagggcac ccagaccatg agaatcaagg tcgtcgaggg
      181 cggccctctc cccttcgcct tcgacatcct ggctaccagc ttcatgtacg gcagcagaac
      241 cttcatcaag caccctccgg gcatccccga cttctttaag cagtccttcc ctgagggctt
      301 cacatgggag agagtcacca catacgaaga cgggggcgtg ctgaccgcta cccaggacac
      361 cagcctccag gacggctgcc tcatctacaa cgtcaaggtt agaggggtga acttcccagc
      421 caacggccct gtgatgcaga agaaaacact cggctgggag gcctccaccg agacgatgta
      481 ccccgctgac ggcggcctgg aaggcgcatg tgacatggcc ctgaagctcg tgggcggggg
      541 ccacctgatc tgcaaccttg agaccacata cagatccaag aaacccgcta cgaacctcaa
      601 gatgcccggc gtctacaacg tggaccacag actggaaaga atcaaggagg ccgacgatga
      661 gacctacgtc gagcagcacg aggtggctgt ggccagatac tctactggtg gcgctggtga
      721 tggaggtaaa ggtggaggag gttccggact cagatctggc agcggtggag gcttggagga
      781 gccggaggcc gcgacgcgga cagccgcggc cgtggattgc aaagacagac ccgggttccc
      841 agtgaagagg ttaatacaag cccgcttgcc cttcaaacgc ctgaaccttg ttcccaagga
      901 gaaagttgag gaagacacat caccaaaagc agctgtggaa agcaaagtcc cggatctaca
      961 attgtctttg ggcacttttg aaagccagtg tcacacaggc tctcacgtag gcttgagtac
     1021 aaagttggtc ggtggccagg gccccattga tagcttcttg agggcaacaa tcaagccagt
     1081 accgagtgtg gtcattatcg acctgacgga gaactgcagt gacattccag acagccctga
     1141 gggccacagt gaactaagtc ctgacactgc tggtgtggtc accacagtgg aaggagctgc
     1201 caagcagcag gagcactctg cagcagagct gtgtctccta gagacccctt cagacatcac
     1261 ttgtcacatg gaagaggagc ctgggagtcc aggagaccca aagaggacgg gtgactgtca
     1321 ggctggttca ctacagagct gtccggagct gaccccaggt tctaggacat gccccaccaa
     1381 ggagttatca agctggagca aggcagggga tctcctattt atagagaaag tgcctgtggt
     1441 ggtgctagag gacatcctgg ccactaagcc ctccattgcc tctcttccca tgatgtccct
     1501 ggataggagt gtcacttcag agagtgaaat actggagtcc tgccccgagg atgactccat
     1561 actgagccac tcctccacca actcctcttc tccaaccagc tctcctgagg gaccatccac
     1621 acccccagaa catcggggtg ggagaagctc cccctccacc ccagcctgca gagttgccaa
     1681 gaactttgtc aaaggctcca cagagaaggg caggagcaaa ctgcacagag atagagaaca
     1741 gcagagggaa gagaaggaaa agctgaggga agagatacga cgagccaagg aggaggcacg
     1801 gaagaagaag gaggaggaga aggagctgaa ggaaaaggag cggagggaaa agcgggagaa
     1861 agatgagaag gagaaggcgg agaagcagcg gctaaaggag gaaaaacgca aggagcggca
     1921 ggaagctctg gaggctaaac tggaggagaa gaggaagaag gaggaagaga agcggttacg
     1981 ggaggaagag aagcggttac gggaagaaga gaagcgcatt aaggcagaaa aggcagagat
     2041 cactcgattc ttccagaaac cgaagactcc gcaggctccc aagaccctgg ccggttcctg
     2101 tggtaagttt gccccctttg aaatcaaaga acacatggtg ctggctccac ggtgccgagc
     2161 tgccttggat caagacctgt gcgatcagct ggaccagctc ctgcagcagc agagtgtggc
     2221 aagcaccttc ctcagtgacc taaaaagccg gctgcccttg agatcggggc ccacccgggt
     2281 ctgtggccat gacacagaca tcatgaacag ggatgtggtc attgtggaga gcagcaaggt
     2341 ggacggtgtg tctgaaagga agaagtttgg ccgcatgaag cttctgcagt tctcagagaa
     2401 ccaccggcca gcatactggg ggacgtggaa caagaagaca gccatcatcc gcccccggaa
     2461 cccctgggcc caggacaaag accttcttga ctatgaggta gacagtgacg atgagtggga
     2521 agaggaggaa cctggggagt ctttgtccca cagtgagggg gatgaggatg acgatgtggg
     2581 ggaagatgag gatgaggacg atggcttctt tgtgccccat gggtacttat ctgaagatga
     2641 aggcgtgact gaggagtgtg cagaccctga gaaccacaaa gtacaccaga agctgaaggc
     2701 caaggagtgg gatgagctcc tagccaaggg caagcgcttt cgtgtgctgc agcctgtgca
     2761 cgtgggatgt gtatgggcat cagaggcagc caactgcacg agttccgact tgaagctgct
     2821 gcagcagttc actgcatgcc tgctggatgt ggcatcccct gatgagccag aacccggggc
     2881 ctccaggagg gaaaagaggg accagcatat cctggcccag ttgcttccgc tgctacatgg
     2941 caatgtgaat gggagcaagg tgatcatcca tgaattccag gagcaatgcc gcaggggcct
     3001 gctgaccctt ccaagtccca ccccacacct gcagatgccc aacttggagg atgctgttgc
     3061 tgtgccatcc aaggccaggc tgaagcgcct tatctctgag aactctgcct atgagaagcg
     3121 gcccaacttc cgtatgtgct ggtatgtgca cccagaggtg ctcaagagct ttggccagga
     3181 gtgcctgcca gtgccctgcc agtggaccta catcaccacc atgccctcag cacccaggga
     3241 agacagtggc agtgcttcta ctgaggggcc tggccagagc acacctatgc tactcaagcg
     3301 gaagccagcg gccaccatgt gcatcacgca gtttatgaag aagcggcgct atgatggaca
     3361 ggttgggtct ggggacatgg atggcttcca ggcagacaca gaagaggatg aagaggatga
     3421 cacagactgt atgatcatag atgtgccaga tgttgggagt gacgtgtcag aagctcccat
     3481 acctgctccc acactttgca agtgaggatc caccggatct agataactga tcataatcag
     3541 ccataccaca tt
//
