LOCUS       sequence-87010-e        1384 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane, Excitation: 580, Emission: 608.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Shemiakina II, Ermakova GV, Cranfill PJ, Baird MA, Evans RA, 
            Souslova EA, Staroverov DB, Gorokhovatsky AY, Putintseva EV, 
            Gorodnicheva TV, Chepurnykh TV, Strukova L, Lukyanov S, Zaraisky AG,
            Davidson MW, Chudakov DM, Shcherbo D
  TITLE     A monomeric red fluorescent protein with low cytotoxicity.
  JOURNAL   Nat Commun. 2012;3:1204. doi: 10.1038/ncomms2208.
  PUBMED    23149748
REFERENCE   2  (bases 1 to 1384)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012"; volume: "3:1204. doi"; pages: " 
            10.1038/ncomms2208"
COMMENT     Sequence Label: FusionRed-Caveolin-C-10
FEATURES             Location/Qualifiers
     source          1..1384
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..730
                     /codon_start=1
                     /product="monomeric red fluorescent protein optimized for 
                     use as a fusion tag (Shemiakina et al., 2012)"
                     /label=FusionRed
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMPMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFMYGSRTFIKHPPGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKVRGVNFPANGPVMQKKTLGWEASTETMYPADGGLEGACDMAL
                     KLVGGGHLICNLETTYRSKKPATNLKMPGVYNVDHRLERIKEADDETYVEQHEVAVARY
                     STGGAGDGGK"
ORIGIN
        1 aacgtcagat ccgctagcgc taccggtcgc caccatggtg agcgagctga ttaaggagaa
       61 catgcccatg aagctgtaca tggagggcac cgtgaacaac caccacttca agtgcacatc
      121 cgagggcgaa ggcaagccct acgagggcac ccagaccatg agaatcaagg tcgtcgaggg
      181 cggccctctc cccttcgcct tcgacatcct ggctaccagc ttcatgtacg gcagcagaac
      241 cttcatcaag caccctccgg gcatccccga cttctttaag cagtccttcc ctgagggctt
      301 cacatgggag agagtcacca catacgaaga cgggggcgtg ctgaccgcta cccaggacac
      361 cagcctccag gacggctgcc tcatctacaa cgtcaaggtt agaggggtga acttcccagc
      421 caacggccct gtgatgcaga agaaaacact cggctgggag gcctccaccg agacgatgta
      481 ccccgctgac ggcggcctgg aaggcgcatg tgacatggcc ctgaagctcg tgggcggggg
      541 ccacctgatc tgcaaccttg agaccacata cagatccaag aaacccgcta cgaacctcaa
      601 gatgcccggc gtctacaacg tggaccacag actggaaaga atcaaggagg ccgacgatga
      661 gacctacgtc gagcagcacg aggtggctgt ggccagatac tctactggtg gcgctggtga
      721 tggaggtaaa ggtggaggag gttccggact cagatctggc agcagtggag gttctggggg
      781 caaatacgta gactcggagg gacatctcta caccgttccc atccgggaac agggcaacat
      841 ctacaagccc aacaacaagg ccatggcaga cgagctgagc gagaagcaag tgtacgacgc
      901 gcacaccaag gagatcgacc tggtcaaccg cgaccctaaa cacctcaacg atgacgtggt
      961 caagattgac tttgaagatg tgattgcaga accagaaggg acacacagtt ttgacggcat
     1021 ttggaaggcc agcttcacca ccttcactgt gacgaaatac tggttttacc gcttgctgtc
     1081 tgccctcttt ggcatcccga tggcactcat ctggggcatt tacttcgcca ttctctcttt
     1141 cctgcacatc tgggcagttg taccatgcat taagagcttc ctgattgaga ttcagtgcat
     1201 cagccgtgtc tattccatct acgtccacac cgtctgtgac ccactctttg aagctgttgg
     1261 gaaaatattc agcaatgtcc gcatcaactt gcagaaagaa atataagaat tctgcagtcg
     1321 acggtaccgc gggcccggga tccaccggat ctagataact gatcataatc agccatacca
     1381 catt
//
