LOCUS       sequence-87011-e        2372 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane, Excitation: 580, Emission: 608.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Shemiakina II, Ermakova GV, Cranfill PJ, Baird MA, Evans RA, 
            Souslova EA, Staroverov DB, Gorokhovatsky AY, Putintseva EV, 
            Gorodnicheva TV, Chepurnykh TV, Strukova L, Lukyanov S, Zaraisky AG,
            Davidson MW, Chudakov DM, Shcherbo D
  TITLE     A monomeric red fluorescent protein with low cytotoxicity.
  JOURNAL   Nat Commun. 2012;3:1204. doi: 10.1038/ncomms2208.
  PUBMED    23149748
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012"; volume: "3:1204. doi"; pages: " 
            10.1038/ncomms2208"
COMMENT     Sequence Label: FusionRed-CD36-C-10
FEATURES             Location/Qualifiers
     source          1..2372
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        44..94
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     46..66
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      133..142
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             139..834
                     /codon_start=1
                     /product="monomeric red fluorescent protein optimized for 
                     use as a fusion tag (Shemiakina et al., 2012)"
                     /label=FusionRed
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMPMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFMYGSRTFIKHPPGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKVRGVNFPANGPVMQKKTLGWEASTETMYPADGGLEGACDMAL
                     KLVGGGHLICNLETTYRSKKPATNLKMPGVYNVDHRLERIKEADDETYVEQHEVAVARY
                     STGGAGDGGK"
ORIGIN
        1 caaatcaacg gacttccaaa atgtcgtaac aactccgccc catgacgcaa atgggcggta
       61 ggcgtgtacg gtggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      121 gcgctaccgg tcgccaccat ggtgagcgag ctgattaagg agaacatgcc catgaagctg
      181 tacatggagg gcaccgtgaa caaccaccac ttcaagtgca catccgaggg cgaaggcaag
      241 ccctacgagg gcacccagac catgagaatc aaggtcgtcg agggcggccc tctccccttc
      301 gccttcgaca tcctggctac cagcttcatg tacggcagca gaaccttcat caagcaccct
      361 ccgggcatcc ccgacttctt taagcagtcc ttccctgagg gcttcacatg ggagagagtc
      421 accacatacg aagacggggg cgtgctgacc gctacccagg acaccagcct ccaggacggc
      481 tgcctcatct acaacgtcaa ggttagaggg gtgaacttcc cagccaacgg ccctgtgatg
      541 cagaagaaaa cactcggctg ggaggcctcc accgagacga tgtaccccgc tgacggcggc
      601 ctggaaggcg catgtgacat ggccctgaag ctcgtgggcg ggggccacct gatctgcaac
      661 cttgagacca catacagatc caagaaaccc gctacgaacc tcaagatgcc cggcgtctac
      721 aacgtggacc acagactgga aagaatcaag gaggccgacg atgagaccta cgtcgagcag
      781 cacgaggtgg ctgtggccag atactctact ggtggcgctg gtgatggagg taaaggtgga
      841 ggaggttccg gactcagatc tggcagcagt ggaggtggct gtgaccggaa ctgtgggctc
      901 atcgctgggg ctgtcattgg tgctgtcctg gctgtgtttg gaggtattct aatgccagtt
      961 ggagacctgc ttatccagaa gacaattaaa aagcaagttg tcctcgaaga aggtacaatt
     1021 gcttttaaaa attgggttaa aacaggcaca gaagtttaca gacagttttg gatctttgat
     1081 gtgcaaaatc cacaggaagt gatgatgaac agcagcaaca ttcaagttaa gcaaagaggt
     1141 ccttatacgt acagagttcg ttttctagcc aaggaaaatg taacccagga cgctgaggac
     1201 aacacagtct ctttcctgca gcccaatggt gccatcttcg aaccttcact atcagttgga
     1261 acagaggctg acaacttcac agttctcaat ctggctgtgg cagctgcatc ccatatctat
     1321 caaaatcaat ttgttcaaat gatcctcaat tcacttatta acaagtcaaa atcttctatg
     1381 ttccaagtca gaactttgag agaactgtta tggggctata gggatccatt tttgagtttg
     1441 gttccgtacc ctgttactac cacagttggt ctgttttatc cttacaacaa tactgcagat
     1501 ggagtttata aagttttcaa tggaaaagat aacataagta aagttgccat aatcgacaca
     1561 tataaaggta aaaggaatct gtcctattgg gaaagtcact gcgacatgat taatggtaca
     1621 gatgcagcct catttccacc ttttgttgag aaaagccagg tattgcagtt cttttcttct
     1681 gatatttgca ggtcaatcta tgctgtattt gaatccgacg ttaatctgaa aggaatccct
     1741 gtgtatagat ttgttcttcc atccaaggcc tttgcctctc cagttgaaaa cccagacaac
     1801 tattgtttct gcacagaaaa aattatctca aaaaattgta catcatatgg tgtgctagac
     1861 atcagcaaat gcaaagaagg gagacctgtg tacatttcac ttcctcattt tctgtatgca
     1921 agtcctgatg tttcagaacc tattgatgga ttaaacccaa atgaagaaga acataggaca
     1981 tacttggata ttgaacctat aactggattc actttacaat ttgcaaaacg gctgcaggtc
     2041 aacctattgg tcaagccatc agaaaaaatt caagtattaa agaatctgaa gaggaactat
     2101 attgtgccta ttctttggct taatgagact gggaccattg gtgatgagaa ggcaaacatg
     2161 ttcagaagtc aagtaactgg aaaaataaac ctccttggcc tgatagaaat gatcttactc
     2221 agtgttggtg tggtgatgtt tgttgctttt atgatttcat attgtgcatg cagatcgaaa
     2281 acaataaaat aagaattctg cagtcgacgg taccgcgggc ccgggatcca ccggatctaa
     2341 gataactgat cataatcagc cataccacat tt
//
