LOCUS       sequence-87014-e        2411 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane/Vesicles, Excitation: 580, Emission: 608.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Shemiakina II, Ermakova GV, Cranfill PJ, Baird MA, Evans RA, 
            Souslova EA, Staroverov DB, Gorokhovatsky AY, Putintseva EV, 
            Gorodnicheva TV, Chepurnykh TV, Strukova L, Lukyanov S, Zaraisky AG,
            Davidson MW, Chudakov DM, Shcherbo D
  TITLE     A monomeric red fluorescent protein with low cytotoxicity.
  JOURNAL   Nat Commun. 2012;3:1204. doi: 10.1038/ncomms2208.
  PUBMED    23149748
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012"; volume: "3:1204. doi"; pages: " 
            10.1038/ncomms2208"
COMMENT     Sequence Label: FusionRed-C-Src-7
FEATURES             Location/Qualifiers
     source          1..2411
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             53..94
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     regulatory      1667..1676
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1673..2371
                     /codon_start=1
                     /product="monomeric red fluorescent protein optimized for 
                     use as a fusion tag (Shemiakina et al., 2012)"
                     /label=FusionRed
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMPMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFMYGSRTFIKHPPGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKVRGVNFPANGPVMQKKTLGWEASTETMYPADGGLEGACDMAL
                     KLVGGGHLICNLETTYRSKKPATNLKMPGVYNVDHRLERIKEADDETYVEQHEVAVARY
                     STGGAGDGGK"
ORIGIN
        1 tcagatccgc tagcgctacc ggactcagat ctcgagctca agcttcgaat tcatggggag
       61 cagcaagagc aagcccaagg accccagcca gcgccggcgc agcctggagc cacccgacag
      121 cacccaccac gggggattcc cagcctcgca gacccccaac aagacagcag cccccgacac
      181 gcaccgcacc cccagccgct cctttgggac cgtggccacc gagcccaagc tcttcggggg
      241 cttcaacact tctgacaccg tcacgtcgcc gcagcgtgcc ggggcactgg ctggcggcgt
      301 caccactttc gtggctctct atgactacga gtcccggact gaaacggact tgtccttcaa
      361 gaaaggagaa cgcctgcaga ttgtcaacaa cacggaaggt gactggtggc tggctcattc
      421 cctcactaca ggacagacgg gctacatccc cagtaactat gtcgcgccct cagactccat
      481 ccaggctgaa gagtggtact ttgggaagat cactcgtcgg gagtccgagc ggctgctgct
      541 caaccccgaa aacccccggg gaaccttctt ggtccgggag agcgagacga caaaaggtgc
      601 ctattgcctc tccgtttctg actttgacaa cgccaagggg ctcaatgtga agcactacaa
      661 gatccgcaag ctggacagcg gcggcttcta catcacctca cgcacacagt tcagcagcct
      721 gcagcagctg gtggcctact actccaaaca tgctgatggc ttgtgccacc gcctgaccaa
      781 cgtctgcccc acgtccaagc cccagaccca gggactcgcc aaggacgcgt gggaaatccc
      841 ccgggagtcg ctgcggctgg aggtgaagct ggggcagggc tgctttggag aggtctggat
      901 ggggacctgg aacggcacca ccagagtggc cataaagact ctgaagcccg gcaccatgtc
      961 cccggaggcc ttcctgcagg aagcccaagt gatgaagaag ctccggcatg agaagctggt
     1021 tcagctgtac gcagtggtgt cggaagagcc catctacatc gtcactgagt acatgagcaa
     1081 ggggagcctc ctggatttcc tgaagggaga gatgggcaag tacctgcggc tgccacagct
     1141 cgtcgatatg gctgctcaga ttgcatccgg catggcctat gtggagagga tgaactacgt
     1201 gcaccgagac ctgcgggcgg ccaacatcct ggtgggggag aacctggtgt gcaaggtggc
     1261 tgactttggg ctggcacgcc tcatcgagga caacgagtac acagcacggc aaggtgccaa
     1321 gttccccatc aagtggacag cccccgaggc agccctctat ggccggttca ccatcaagtc
     1381 ggatgtctgg tccttcggca tcctgctgac tgagctgacc accaagggcc gggtgccata
     1441 cccagggatg gtcaacaggg aggtgctgga ccaggtggag aggggctacc gcatgccctg
     1501 cccgcccgag tgccccgagt cgctgcatga cctcatgtgc cagtgctggc ggaaggaccc
     1561 tgaggagcgg cccacttttg agtacctgca ggccttcctg gaggactact tcacctcgac
     1621 agagccccag taccagcctg gagagaacct acgggatcca ccggtcgcca ccatggtgag
     1681 cgagctgatt aaggagaaca tgcccatgaa gctgtacatg gagggcaccg tgaacaacca
     1741 ccacttcaag tgcacatccg agggcgaagg caagccctac gagggcaccc agaccatgag
     1801 aatcaaggtc gtcgagggcg gccctctccc cttcgccttc gacatcctgg ctaccagctt
     1861 catgtacggc agcagaacct tcatcaagca ccctccgggc atccccgact tctttaagca
     1921 gtccttccct gagggcttca catgggagag agtcaccaca tacgaagacg ggggcgtgct
     1981 gaccgctacc caggacacca gcctccagga cggctgcctc atctacaacg tcaaggttag
     2041 aggggtgaac ttcccagcca acggccctgt gatgcagaag aaaacactcg gctgggaggc
     2101 ctccaccgag acgatgtacc ccgctgacgg cggcctggaa ggcgcatgtg acatggccct
     2161 gaagctcgtg ggcgggggcc acctgatctg caaccttgag accacataca gatccaagaa
     2221 acccgctacg aacctcaaga tgcccggcgt ctacaacgtg gaccacagac tggaaagaat
     2281 caaggaggcc gacgatgaga cctacgtcga gcagcacgag gtggctgtgg ccagatactc
     2341 tactggtggc gctggtgatg gaggtaaatg agcggccgcg actctagatc ataatcagcc
     2401 ataccacatt g
//
