LOCUS       sequence-87024-e        2272 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 580, Emission: 608.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Shemiakina II, Ermakova GV, Cranfill PJ, Baird MA, Evans RA, 
            Souslova EA, Staroverov DB, Gorokhovatsky AY, Putintseva EV, 
            Gorodnicheva TV, Chepurnykh TV, Strukova L, Lukyanov S, Zaraisky AG,
            Davidson MW, Chudakov DM, Shcherbo D
  TITLE     A monomeric red fluorescent protein with low cytotoxicity.
  JOURNAL   Nat Commun. 2012;3:1204. doi: 10.1038/ncomms2208.
  PUBMED    23149748
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012"; volume: "3:1204. doi"; pages: " 
            10.1038/ncomms2208"
COMMENT     Sequence Label: FusionRed-Fascin-C-10
FEATURES             Location/Qualifiers
     source          1..2272
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             24..719
                     /codon_start=1
                     /product="monomeric red fluorescent protein optimized for 
                     use as a fusion tag (Shemiakina et al., 2012)"
                     /label=FusionRed
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMPMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFMYGSRTFIKHPPGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKVRGVNFPANGPVMQKKTLGWEASTETMYPADGGLEGACDMAL
                     KLVGGGHLICNLETTYRSKKPATNLKMPGVYNVDHRLERIKEADDETYVEQHEVAVARY
                     STGGAGDGGK"
ORIGIN
        1 cgctagcgct accggtcgcc accatggtga gcgagctgat taaggagaac atgcccatga
       61 agctgtacat ggagggcacc gtgaacaacc accacttcaa gtgcacatcc gagggcgaag
      121 gcaagcccta cgagggcacc cagaccatga gaatcaaggt cgtcgagggc ggccctctcc
      181 ccttcgcctt cgacatcctg gctaccagct tcatgtacgg cagcagaacc ttcatcaagc
      241 accctccggg catccccgac ttctttaagc agtccttccc tgagggcttc acatgggaga
      301 gagtcaccac atacgaagac gggggcgtgc tgaccgctac ccaggacacc agcctccagg
      361 acggctgcct catctacaac gtcaaggtta gaggggtgaa cttcccagcc aacggccctg
      421 tgatgcagaa gaaaacactc ggctgggagg cctccaccga gacgatgtac cccgctgacg
      481 gcggcctgga aggcgcatgt gacatggccc tgaagctcgt gggcgggggc cacctgatct
      541 gcaaccttga gaccacatac agatccaaga aacccgctac gaacctcaag atgcccggcg
      601 tctacaacgt ggaccacaga ctggaaagaa tcaaggaggc cgacgatgag acctacgtcg
      661 agcagcacga ggtggctgtg gccagatact ctactggtgg cgctggtgat ggaggtaaag
      721 gtggaggagg ttccggagct ggtagtggtg gaagcgcagg aaccgccaac ggcacagccg
      781 aggcggtgca gatccagttc ggcctcatca actgcggcaa caagtacctg acggccgagg
      841 cgttcgggtt caaggtgaac gcgtccgcca gcagcctgaa gaagaagcag atctggacgc
      901 tggagcagcc ccctgacgag gcgggcagcg cggccgtgtg cctgcgcagc cacctgggcc
      961 gctacctggc ggcggacaag gacggcaacg tgacctgcga gcgcgaggtg cccggtcccg
     1021 actgccgttt cctcatcgtg gcgcacgacg acggtcgctg gtcgctgcag tccgaggcgc
     1081 accggcgcta cttcggcggc accgaggacc gcctgtcctg cttcgcgcag acggtgtccc
     1141 ccgccgagaa gtggagcgtg cacatcgcca tgcaccctca ggtcaacatc tacagcgtca
     1201 cccgtaagcg ctacgcgcac ctgagcgcgc ggccggccga cgagatcgcc gtggaccgcg
     1261 acgtgccctg gggcgtcgac tcgctcatca ccctcgcctt ccaggaccag cgctacagcg
     1321 tgcagaccgc cgaccaccgc ttcctgcgcc acgacgggcg cctggtggcg cgccccgagc
     1381 cggccactgg ctacacgctg gagttccgct ccggcaaggt ggccttccgc gactgcgagg
     1441 gccgttacct ggcgccgtcg gggcccagcg gcacgctcaa ggcgggcaag gccaccaagg
     1501 tgggcaagga cgagctcttt gctctggagc agagctgcgc ccaggtcgtg ctgcaggcgg
     1561 ccaacgagag gaacgtgtcc acgcgccagg gtatggacct gtctgccaat caggacgagg
     1621 agaccgacca ggagaccttc cagctggaga tcgaccgcga caccaaaaag tgtgccttcc
     1681 gtacccacac gggcaagtac tggacgctga cggccaccgg gggcgtgcag tccaccgcct
     1741 ccagcaagaa tgccagctgc tactttgaca tcgagtggcg tgaccggcgc atcacactga
     1801 gggcgtccaa tggcaagttt gtgacctcca agaagaatgg gcagctggcc gcctcggtgg
     1861 agacggcagg ggactcagag ctcttcctca tgaagctcat caaccgcccc atcatcgtgt
     1921 tccgcgggga gcatggcttc atcggctgcc gcaaggtcac gggcaccctg gacgccaacc
     1981 gctccagcta tgacgtcttc cagctggagt tcaacgatgg cgcctacaac atcaaagact
     2041 ccacaggcaa atactggacg gtgggcagtg actccgtggt caccagcagc ggcgacactc
     2101 ctgtggactt cttcttcgag ttctgcgact ataacaaggt ggccatcaag gtgggcgggc
     2161 gctacctgaa gggcgaccac gcaggcgtcc tgaaggcctc ggcggaaacc gtggaccccg
     2221 cctcgctctg ggagtactag ggatccaccg gatctagata actgatcata at
//
