LOCUS       sequence-87037-e        2698 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 580, Emission: 608.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Shemiakina II, Ermakova GV, Cranfill PJ, Baird MA, Evans RA, 
            Souslova EA, Staroverov DB, Gorokhovatsky AY, Putintseva EV, 
            Gorodnicheva TV, Chepurnykh TV, Strukova L, Lukyanov S, Zaraisky AG,
            Davidson MW, Chudakov DM, Shcherbo D
  TITLE     A monomeric red fluorescent protein with low cytotoxicity.
  JOURNAL   Nat Commun. 2012;3:1204. doi: 10.1038/ncomms2208.
  PUBMED    23149748
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012"; volume: "3:1204. doi"; pages: " 
            10.1038/ncomms2208"
COMMENT     Sequence Label: FusionRed-PlastinC-10
FEATURES             Location/Qualifiers
     source          1..2698
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             34..729
                     /codon_start=1
                     /product="monomeric red fluorescent protein optimized for 
                     use as a fusion tag (Shemiakina et al., 2012)"
                     /label=FusionRed
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMPMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFMYGSRTFIKHPPGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKVRGVNFPANGPVMQKKTLGWEASTETMYPADGGLEGACDMAL
                     KLVGGGHLICNLETTYRSKKPATNLKMPGVYNVDHRLERIKEADDETYVEQHEVAVARY
                     STGGAGDGGK"
     RBS             2103..2111
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatggtga gcgagctgat taaggagaac
       61 atgcccatga agctgtacat ggagggcacc gtgaacaacc accacttcaa gtgcacatcc
      121 gagggcgaag gcaagcccta cgagggcacc cagaccatga gaatcaaggt cgtcgagggc
      181 ggccctctcc ccttcgcctt cgacatcctg gctaccagct tcatgtacgg cagcagaacc
      241 ttcatcaagc accctccggg catccccgac ttctttaagc agtccttccc tgagggcttc
      301 acatgggaga gagtcaccac atacgaagac gggggcgtgc tgaccgctac ccaggacacc
      361 agcctccagg acggctgcct catctacaac gtcaaggtta gaggggtgaa cttcccagcc
      421 aacggccctg tgatgcagaa gaaaacactc ggctgggagg cctccaccga gacgatgtac
      481 cccgctgacg gcggcctgga aggcgcatgt gacatggccc tgaagctcgt gggcgggggc
      541 cacctgatct gcaaccttga gaccacatac agatccaaga aacccgctac gaacctcaag
      601 atgcccggcg tctacaacgt ggaccacaga ctggaaagaa tcaaggaggc cgacgatgag
      661 acctacgtcg agcagcacga ggtggctgtg gccagatact ctactggtgg cgctggtgat
      721 ggaggtaaag gtggaggagg ttccggaagc ggctccggga gcggaagcgg ggaaaacagt
      781 actactacca tttctcggga ggagcttgaa gaactacaag aggcatttaa taaaatagat
      841 attgacaata gtgggtatgt cagtgactat gaacttcaag acctgtttaa ggaagcaagc
      901 cttcctctgc ctggctacaa ggtgcgcgag attgtggaga aaattctatc agttgctgac
      961 agcaacaaag atggcaaaat cagttttgaa gagtttgtgt cactaatgca agaattaaaa
     1021 agcaaagata tcagcaaaac attccgaaaa ataattaaca agagggaagg gattactgct
     1081 attggaggaa cttcaactat ttccagtgag ggcacacagc attcttattc agaggaagaa
     1141 aaagtggctt ttgttaactg gataaacaaa gccctggaga atgaccctga ctgtaagcat
     1201 cttataccca tgaatcccaa tgatgatagt cttttcaagt cacttgcaga tggcatcctt
     1261 ctttgcaaaa tgatcaactt atctgaacca gatacaattg atgaaagagc catcaataag
     1321 aaaaagctca cgccattcac tatttctgaa aatttaaacc tagctctgaa ttctgcctca
     1381 gccattggtt gtacagtggt caacattggt gcatcagatc tcaaagaagg aaaacctcac
     1441 ttggtcttgg gacttctctg gcagatcatc aaagttggcc tttttgctga tattgagatt
     1501 tccaggaatg aagctctgat tgcattgtta aatgaaggtg aggaactaga ggagctgatg
     1561 aagctttctc ccgaggaatt actgctgcga tgggtgaact accatctgac caatgcagga
     1621 tggcatacca tcagcaactt cagccaagac attaaggact cgagagccta ttttcatctg
     1681 cttaatcaga ttgcccctaa aggtggggaa gatggacctg ccattgccat tgacctttca
     1741 ggaattaatg agacaaatga cctgaagcgt gctggactca tgcttcaaga agcagataaa
     1801 ctgggctgca aacagtttgt tactcctgca gatgtggttt caggcaatcc taaacttaat
     1861 ttagcttttg tagctaattt gtttaacaca tacccgtgcc tgcacaagcc gaataataat
     1921 gacatcgata tgaatttact ggaaggagag agcaaggaag agagaacatt tcggaactgg
     1981 atgaattcct tgggagtcaa cccatacatt aatcatttgt acagtgacct tgcagatgct
     2041 ttagtgatct ttcagctcta tgagatgatc cgagtgccag tcaactggag ccatgtcaac
     2101 aaacctcctt atcctgccct tggagggaac atgaagaaga ttgaaaactg taactatgca
     2161 gtggaacttg ggaagaacaa ggccaaattc tccttggttg gcattgctgg gcaggaccta
     2221 aatgaaggga attcaacact taccctggca ttggtatggc agctgatgag aaggtacaca
     2281 ttgaatgtgt tatcggatct tggagagggt gaaaaagtaa atgatgaaat tataattaaa
     2341 tgggtcaatc agactcttaa aagtgcaaac aaaaagactt ctatttccag cttcaaggat
     2401 aaatctataa gcacaagttt acctgtccta gatttaatag atgccattgc accaaatgca
     2461 gttcgtcaag aaatgatcag gagagaaaac ttatctgatg aggacaagct gaacaatgct
     2521 aaatacgcca tttcagttgc tcgaaagatc ggtgcccgga tatatgcatt acctgatgac
     2581 ctcgtagaag tgaaaccaaa gatggttatg acggtgtttg catgcttaat gggaaaagga
     2641 ctgaacagaa taaaataagg atccaccgga tctagataac tgatcataat cagccata
//
