LOCUS       sequence-87038-e        1395 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Peroxisomes, Excitation: 580, Emission: 608.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Shemiakina II, Ermakova GV, Cranfill PJ, Baird MA, Evans RA, 
            Souslova EA, Staroverov DB, Gorokhovatsky AY, Putintseva EV, 
            Gorodnicheva TV, Chepurnykh TV, Strukova L, Lukyanov S, Zaraisky AG,
            Davidson MW, Chudakov DM, Shcherbo D
  TITLE     A monomeric red fluorescent protein with low cytotoxicity.
  JOURNAL   Nat Commun. 2012;3:1204. doi: 10.1038/ncomms2208.
  PUBMED    23149748
REFERENCE   2  (bases 1 to 1395)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012"; volume: "3:1204. doi"; pages: " 
            10.1038/ncomms2208"
COMMENT     Sequence Label: FusionRed-PMP-N-10
FEATURES             Location/Qualifiers
     source          1..1395
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      652..661
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             658..1356
                     /codon_start=1
                     /product="monomeric red fluorescent protein optimized for 
                     use as a fusion tag (Shemiakina et al., 2012)"
                     /label=FusionRed
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMPMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFMYGSRTFIKHPPGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKVRGVNFPANGPVMQKKTLGWEASTETMYPADGGLEGACDMAL
                     KLVGGGHLICNLETTYRSKKPATNLKMPGVYNVDHRLERIKEADDETYVEQHEVAVARY
                     STGGAGDGGK"
ORIGIN
        1 ggtcagaatc cggctagcgc taccggactc agatctgcca ccatggcgcc ggccgcgtcc
       61 aggctgcggg ccgaagccgg gctcggggcg ctgccgcggc gggcgctcgc ccagtacttg
      121 ctcttcctgc ggctctaccc ggtgctcacc aaggcggcca ccagtggcat tttgtcagca
      181 cttgggaact tcctggccca gatgattgag aagaagcgga aaaaagaaaa ctctagaagt
      241 ctggatgtcg gtgggcctct gagatatgcc gtttacgggt tcttcttcac agggccgctg
      301 agtcacttct tctacttctt catggaacat tggatccctc ctgaggtccc cctggcaggg
      361 ctcaggaggc ttctcctgga ccgcctcgtc tttgcaccgg ccttcctcat gttgttcttc
      421 ctcatcatga actttctgga ggggaaagac gcctcagcct tcgccgccaa gatgaggggg
      481 ggcttctggc cggcgctgag gatgaactgg cgggtgtgga cgccactaca gttcatcaac
      541 atcaactacg tccctctgaa gttccgggtg ctcttcgcca acctggcagc tctgttctgg
      601 tatgcctacc tggcctcctt ggggaagagc ggctccggta gcggaccggt cgccaccatg
      661 gtgagcgagc tgattaagga gaacatgccc atgaagctgt acatggaggg caccgtgaac
      721 aaccaccact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc
      781 atgagaatca aggtcgtcga gggcggccct ctccccttcg ccttcgacat cctggctacc
      841 agcttcatgt acggcagcag aaccttcatc aagcaccctc cgggcatccc cgacttcttt
      901 aagcagtcct tccctgaggg cttcacatgg gagagagtca ccacatacga agacgggggc
      961 gtgctgaccg ctacccagga caccagcctc caggacggct gcctcatcta caacgtcaag
     1021 gttagagggg tgaacttccc agccaacggc cctgtgatgc agaagaaaac actcggctgg
     1081 gaggcctcca ccgagacgat gtaccccgct gacggcggcc tggaaggcgc atgtgacatg
     1141 gccctgaagc tcgtgggcgg gggccacctg atctgcaacc ttgagaccac atacagatcc
     1201 aagaaacccg ctacgaacct caagatgccc ggcgtctaca acgtggacca cagactggaa
     1261 agaatcaagg aggccgacga tgagacctac gtcgagcagc acgaggtggc tgtggccaga
     1321 tactctactg gtggcgctgg tgatggaggt aaatgagcgg ccgcgactct agatcataat
     1381 cagccatacc acatt
//
