LOCUS       sequence-87040-e         961 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Golgi TGN, Excitation: 580, Emission: 608.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Shemiakina II, Ermakova GV, Cranfill PJ, Baird MA, Evans RA, 
            Souslova EA, Staroverov DB, Gorokhovatsky AY, Putintseva EV, 
            Gorodnicheva TV, Chepurnykh TV, Strukova L, Lukyanov S, Zaraisky AG,
            Davidson MW, Chudakov DM, Shcherbo D
  TITLE     A monomeric red fluorescent protein with low cytotoxicity.
  JOURNAL   Nat Commun. 2012;3:1204. doi: 10.1038/ncomms2208.
  PUBMED    23149748
REFERENCE   2  (bases 1 to 961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012"; volume: "3:1204. doi"; pages: " 
            10.1038/ncomms2208"
COMMENT     Sequence Label: FusionRed-Sit-N-15
FEATURES             Location/Qualifiers
     source          1..961
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      217..226
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             223..921
                     /codon_start=1
                     /product="monomeric red fluorescent protein optimized for 
                     use as a fusion tag (Shemiakina et al., 2012)"
                     /label=FusionRed
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMPMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFMYGSRTFIKHPPGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKVRGVNFPANGPVMQKKTLGWEASTETMYPADGGLEGACDMAL
                     KLVGGGHLICNLETTYRSKKPATNLKMPGVYNVDHRLERIKEADDETYVEQHEVAVARY
                     STGGAGDGGK"
ORIGIN
        1 aatccgctag cgctaccgga ctcagatctc gagctcgcca ccatgattca caccaacctg
       61 aagaaaaagt tcagctgctg cgtcctggtc tttcttctgt ttgcagtcat ctgtgtgtgg
      121 aaggaaaaga agaaagggag ttactatgat tcctttaaat tgcaaaccaa ggaattcctg
      181 cagtcgacgg taccgcgggc ccgggatcca ccggtcgcca ccatggtgag cgagctgatt
      241 aaggagaaca tgcccatgaa gctgtacatg gagggcaccg tgaacaacca ccacttcaag
      301 tgcacatccg agggcgaagg caagccctac gagggcaccc agaccatgag aatcaaggtc
      361 gtcgagggcg gccctctccc cttcgccttc gacatcctgg ctaccagctt catgtacggc
      421 agcagaacct tcatcaagca ccctccgggc atccccgact tctttaagca gtccttccct
      481 gagggcttca catgggagag agtcaccaca tacgaagacg ggggcgtgct gaccgctacc
      541 caggacacca gcctccagga cggctgcctc atctacaacg tcaaggttag aggggtgaac
      601 ttcccagcca acggccctgt gatgcagaag aaaacactcg gctgggaggc ctccaccgag
      661 acgatgtacc ccgctgacgg cggcctggaa ggcgcatgtg acatggccct gaagctcgtg
      721 ggcgggggcc acctgatctg caaccttgag accacataca gatccaagaa acccgctacg
      781 aacctcaaga tgcccggcgt ctacaacgtg gaccacagac tggaaagaat caaggaggcc
      841 gacgatgaga cctacgtcga gcagcacgag gtggctgtgg ccagatactc tactggtggc
      901 gctggtgatg gaggtaaatg agcggccgcg actctagatc ataatcagcc ataccacatt
      961 t
//
