LOCUS       sequence-87042-e        1917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 580, Emission: 608.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Shemiakina II, Ermakova GV, Cranfill PJ, Baird MA, Evans RA, 
            Souslova EA, Staroverov DB, Gorokhovatsky AY, Putintseva EV, 
            Gorodnicheva TV, Chepurnykh TV, Strukova L, Lukyanov S, Zaraisky AG,
            Davidson MW, Chudakov DM, Shcherbo D
  TITLE     A monomeric red fluorescent protein with low cytotoxicity.
  JOURNAL   Nat Commun. 2012;3:1204. doi: 10.1038/ncomms2208.
  PUBMED    23149748
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012"; volume: "3:1204. doi"; pages: " 
            10.1038/ncomms2208"
COMMENT     Sequence Label: FusionRed-VASP-5
FEATURES             Location/Qualifiers
     source          1..1917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      22..31
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             28..723
                     /codon_start=1
                     /product="monomeric red fluorescent protein optimized for 
                     use as a fusion tag (Shemiakina et al., 2012)"
                     /label=FusionRed
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMPMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFMYGSRTFIKHPPGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKVRGVNFPANGPVMQKKTLGWEASTETMYPADGGLEGACDMAL
                     KLVGGGHLICNLETTYRSKKPATNLKMPGVYNVDHRLERIKEADDETYVEQHEVAVARY
                     STGGAGDGGK"
ORIGIN
        1 gatccgctag cgctaccggt cgccaccatg gtgagcgagc tgattaagga gaacatgccc
       61 atgaagctgt acatggaggg caccgtgaac aaccaccact tcaagtgcac atccgagggc
      121 gaaggcaagc cctacgaggg cacccagacc atgagaatca aggtcgtcga gggcggccct
      181 ctccccttcg ccttcgacat cctggctacc agcttcatgt acggcagcag aaccttcatc
      241 aagcaccctc cgggcatccc cgacttcttt aagcagtcct tccctgaggg cttcacatgg
      301 gagagagtca ccacatacga agacgggggc gtgctgaccg ctacccagga caccagcctc
      361 caggacggct gcctcatcta caacgtcaag gttagagggg tgaacttccc agccaacggc
      421 cctgtgatgc agaagaaaac actcggctgg gaggcctcca ccgagacgat gtaccccgct
      481 gacggcggcc tggaaggcgc atgtgacatg gccctgaagc tcgtgggcgg gggccacctg
      541 atctgcaacc ttgagaccac atacagatcc aagaaacccg ctacgaacct caagatgccc
      601 ggcgtctaca acgtggacca cagactggaa agaatcaagg aggccgacga tgagacctac
      661 gtcgagcagc acgaggtggc tgtggccaga tactctactg gtggcgctgg tgatggaggt
      721 aaaggtggag gaggttccgg actcagatct atgagcgaga cggtcatctg ttccagccgg
      781 gctactgtga tgctttatga tgacagcaac aagcgatggc tccctgctgg cactggtccc
      841 caggccttca gccgcgtcca gatataccac aaccccactg ctaactcctt ccgagttgtt
      901 ggccgcaaga tgcagccgga tcagcaggtg gttatcaact gtgccatcat tcggggtgtc
      961 aagtacaatc aggccactcc catcttccat cagtggcgag atgcccgcca ggtctggggc
     1021 ctcaacttcg gcagcaagga ggacgccata cagtttgcaa caggcatggc caacgcccta
     1081 gaggccttgg aaggaggtgg gcctccccca gccccagcac cccctgcctg gtctgcccag
     1141 aatggtccct ccccagagga gctggaacaa cagaaaaggc agccggagca tatggagcgc
     1201 cgggtctcca atgcaggagg cccacctgct cccccagctg ggggccctcc tccacctcca
     1261 ggacctcccc ctcctccagg tcccccccca cccccaggtc tgccctcctc aggggtatct
     1321 ggggcaggtc atggagcagg ggcagcccca ccccctgcac ccccactccc tacaacacag
     1381 ggccccaata gtgggggttc cggggcccca ggcctggctg ctgccattgc tggagccaaa
     1441 ctcaggaaag tgagcaagca ggaggaggcc tctggggggc ccctggcccc caaagctgag
     1501 aacagtcgaa gcactggtgg ggggcttatg gaagagatga acgccatgct ggcccggaga
     1561 agaaaagcca cacaggttgg ggagaagccc cccaaagacg agtcagccag tgaggagtca
     1621 gaggcccgac tccctgccca gagtgaacct gtgagaagac cctgggagaa gaacagcaca
     1681 accttgccaa ggatgaagtc gtcctcttct gtgactacct ccgaggccca cccctcgacg
     1741 ccctgctcca gtgatgactc cgacttggag agggtgaagc aggagcttct ggaagaggtg
     1801 cggaaggagc tacagaaaat gaaagaggaa atcatcgaag tctttgtcca ggagctgagg
     1861 aagcggggtt ctccttgagc ggccgcgact ctagatcata atcagccata ccacatt
//
