LOCUS       sequence-87050-e        1976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 604, Emission: 659.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Chu J, Haynes RD, Corbel SY, Li P, Gonzalez-Gonzalez E, Burg JS, 
            Ataie NJ, Lam AJ, Cranfill PJ, Baird MA, Davidson MW, Ng HL, Garcia 
            KC, Contag CH, Shen K, Blau HM, Lin MZ
  TITLE     Non-invasive intravital imaging of cellular differentiation with a 
            bright red-excitable fluorescent protein.
  JOURNAL   Nat Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888.
  PUBMED    24633408
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888."
COMMENT     Sequence Label: mCardinal-Actin-C-18
FEATURES             Location/Qualifiers
     source          1..1976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      16..25
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             22..753
                     /codon_start=1
                     /product="far-red fluorescent protein derived from 
                     mNeptune, with further red-shifted spectra"
                     /label=mCardinal
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTTEGEGKPYEGTQTQ
                     RIKVVEGGPLPFAFDILATCFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTVTQDTSLQDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEATTETLYPADGGLEGRCD
                     MALKLVGGGHLHCNLKTTYRSKKPAKNLKMPGVYFVDRRLERIKEADNETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 ctagcgctac cggtcgccac catggtgagc aagggcgagg agctgatcaa ggagaacatg
       61 cacatgaagc tgtacatgga aggcaccgtg aacaaccacc acttcaagtg caccaccgaa
      121 ggggagggca agccctacga gggcacccag acccagagga ttaaggtggt ggagggaggc
      181 cccctgccgt tcgcattcga catcctggcc acctgcttta tgtacgggag caagaccttc
      241 atcaaccaca cccagggcat ccccgatttc tttaagcagt ccttccctga gggcttcaca
      301 tgggagagag tcaccacata cgaagacggg ggcgtgctta ccgttaccca ggacaccagc
      361 ctccaggacg gctgcttgat ctacaacgtc aagctcagag gggtgaactt cccatccaac
      421 ggccctgtga tgcagaagaa aacactcggc tgggaggcca ccaccgagac cctgtacccc
      481 gctgacggcg gcctggaagg cagatgcgac atggccctga agctcgtggg cgggggccac
      541 ctgcactgca acctgaagac cacatacaga tccaagaaac ccgctaagaa cctcaagatg
      601 cccggcgtct actttgtgga ccgcagactg gaaagaatca aggaggccga caatgagacc
      661 tacgtcgagc agcacgaggt ggctgtggcc agatactgcg acctccctag caaactgggg
      721 cacaaactta atggcatgga cgagctgtac aagtccggac tcagatctgg cagcggtgga
      781 ggcagcgcat ccggcggaag cggaagcgat gatgatatcg ccgcgctcgt cgtcgacaac
      841 ggctccggca tgtgcaaggc cggcttcgcg ggcgacgatg ccccccgggc cgtcttcccc
      901 tccatcgtgg ggcgccccag gcaccagggc gtgatggtgg gcatgggtca gaaggattcc
      961 tatgtgggcg acgaggccca gagcaagaga ggcatcctca ccctgaagta ccccatcgag
     1021 cacggcatcg tcaccaactg ggacgacatg gagaaaatct ggcaccacac cttctacaat
     1081 gagctgcgtg tggctcccga ggagcacccc gtgctgctga ccgaggcccc cctgaacccc
     1141 aaggccaacc gcgagaagat gacccagatc atgtttgaga ccttcaacac cccagccatg
     1201 tacgttgcta tccaggctgt gctatccctg tacgcctctg gccgtaccac tggcatcgtg
     1261 atggactccg gtgacggggt cacccacact gtgcccatct acgaggggta tgccctcccc
     1321 catgccatcc tgcgtctgga cctggctggc cgggacctga ctgactacct catgaagatc
     1381 ctcaccgagc gcggctacag cttcaccacc acggccgagc gggaaatcgt gcgtgacatt
     1441 aaggagaagc tgtgctacgt cgccctggac ttcgagcaag agatggccac ggctgcttcc
     1501 agctcctccc tggagaagag ctacgagctg cctgacggcc aggtcatcac cattggcaat
     1561 gagcggttcc gctgccctga ggcactcttc cagccttcct tcctgggcat ggagtcctgt
     1621 ggcatccacg aaactacctt caactccatc atgaagtgtg acgtggacat ccgcaaagac
     1681 ctgtacgcca acacagtgct gtctggcggc accaccatgt accctggcat tgccgacagg
     1741 atgcagaagg agatcactgc cctggcaccc agcacaatga agatcaagat cattgctcct
     1801 cctgagcgca agtactccgt gtggatcggc ggctccatcc tggcctcgct gtccaccttc
     1861 cagcagatgt ggatcagcaa gcaggagtat gacgagtccg gcccctccat cgtccaccgc
     1921 aaatgcttct agggatccac cggatctaga taactgatca taatcagcca taccac
//
