LOCUS       sequence-87052-e        3550 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Chromatin Assembly Factor 1, Excitation: 604, 
            Emission: 659.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3550)
  AUTHORS   Chu J, Haynes RD, Corbel SY, Li P, Gonzalez-Gonzalez E, Burg JS, 
            Ataie NJ, Lam AJ, Cranfill PJ, Baird MA, Davidson MW, Ng HL, Garcia 
            KC, Contag CH, Shen K, Blau HM, Lin MZ
  TITLE     Non-invasive intravital imaging of cellular differentiation with a 
            bright red-excitable fluorescent protein.
  JOURNAL   Nat Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888.
  PUBMED    24633408
REFERENCE   2  (bases 1 to 3550)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888."
COMMENT     Sequence Label: mCardinal-CAF1-C-10
FEATURES             Location/Qualifiers
     source          1..3550
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             24..755
                     /codon_start=1
                     /product="far-red fluorescent protein derived from 
                     mNeptune, with further red-shifted spectra"
                     /label=mCardinal
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTTEGEGKPYEGTQTQ
                     RIKVVEGGPLPFAFDILATCFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTVTQDTSLQDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEATTETLYPADGGLEGRCD
                     MALKLVGGGHLHCNLKTTYRSKKPAKNLKMPGVYFVDRRLERIKEADNETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     protein_bind    2574..2585
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
ORIGIN
        1 cgctagcgct accggtcgcc accatggtga gcaagggcga ggagctgatc aaggagaaca
       61 tgcacatgaa gctgtacatg gaaggcaccg tgaacaacca ccacttcaag tgcaccaccg
      121 aaggggaggg caagccctac gagggcaccc agacccagag gattaaggtg gtggagggag
      181 gccccctgcc gttcgcattc gacatcctgg ccacctgctt tatgtacggg agcaagacct
      241 tcatcaacca cacccagggc atccccgatt tctttaagca gtccttccct gagggcttca
      301 catgggagag agtcaccaca tacgaagacg ggggcgtgct taccgttacc caggacacca
      361 gcctccagga cggctgcttg atctacaacg tcaagctcag aggggtgaac ttcccatcca
      421 acggccctgt gatgcagaag aaaacactcg gctgggaggc caccaccgag accctgtacc
      481 ccgctgacgg cggcctggaa ggcagatgcg acatggccct gaagctcgtg ggcgggggcc
      541 acctgcactg caacctgaag accacataca gatccaagaa acccgctaag aacctcaaga
      601 tgcccggcgt ctactttgtg gaccgcagac tggaaagaat caaggaggcc gacaatgaga
      661 cctacgtcga gcagcacgag gtggctgtgg ccagatactg cgacctccct agcaaactgg
      721 ggcacaaact taatggcatg gacgagctgt acaagtccgg actcagatct ggcagcggtg
      781 gaggcttgga ggagccggag gccgcgacgc ggacagccgc ggccgtggat tgcaaagaca
      841 gacccgggtt cccagtgaag aggttaatac aagcccgctt gcccttcaaa cgcctgaacc
      901 ttgttcccaa ggagaaagtt gaggaagaca catcaccaaa agcagctgtg gaaagcaaag
      961 tcccggatct acaattgtct ttgggcactt ttgaaagcca gtgtcacaca ggctctcacg
     1021 taggcttgag tacaaagttg gtcggtggcc agggccccat tgatagcttc ttgagggcaa
     1081 caatcaagcc agtaccgagt gtggtcatta tcgacctgac ggagaactgc agtgacattc
     1141 cagacagccc tgagggccac agtgaactaa gtcctgacac tgctggtgtg gtcaccacag
     1201 tggaaggagc tgccaagcag caggagcact ctgcagcaga gctgtgtctc ctagagaccc
     1261 cttcagacat cacttgtcac atggaagagg agcctgggag tccaggagac ccaaagagga
     1321 cgggtgactg tcaggctggt tcactacaga gctgtccgga gctgacccca ggttctagga
     1381 catgccccac caaggagtta tcaagctgga gcaaggcagg ggatctccta tttatagaga
     1441 aagtgcctgt ggtggtgcta gaggacatcc tggccactaa gccctccatt gcctctcttc
     1501 ccatgatgtc cctggatagg agtgtcactt cagagagtga aatactggag tcctgccccg
     1561 aggatgactc catactgagc cactcctcca ccaactcctc ttctccaacc agctctcctg
     1621 agggaccatc cacaccccca gaacatcggg gtgggagaag ctccccctcc accccagcct
     1681 gcagagttgc caagaacttt gtcaaaggct ccacagagaa gggcaggagc aaactgcaca
     1741 gagatagaga acagcagagg gaagagaagg aaaagctgag ggaagagata cgacgagcca
     1801 aggaggaggc acggaagaag aaggaggagg agaaggagct gaaggaaaag gagcggaggg
     1861 aaaagcggga gaaagatgag aaggagaagg cggagaagca gcggctaaag gaggaaaaac
     1921 gcaaggagcg gcaggaagct ctggaggcta aactggagga gaagaggaag aaggaggaag
     1981 agaagcggtt acgggaggaa gagaagcggt tacgggaaga agagaagcgc attaaggcag
     2041 aaaaggcaga gatcactcga ttcttccaga aaccgaagac tccgcaggct cccaagaccc
     2101 tggccggttc ctgtggtaag tttgccccct ttgaaatcaa agaacacatg gtgctggctc
     2161 cacggtgccg agctgccttg gatcaagacc tgtgcgatca gctggaccag ctcctgcagc
     2221 agcagagtgt ggcaagcacc ttcctcagtg acctaaaaag ccggctgccc ttgagatcgg
     2281 ggcccacccg ggtctgtggc catgacacag acatcatgaa cagggatgtg gtcattgtgg
     2341 agagcagcaa ggtggacggt gtgtctgaaa ggaagaagtt tggccgcatg aagcttctgc
     2401 agttctcaga gaaccaccgg ccagcatact gggggacgtg gaacaagaag acagccatca
     2461 tccgcccccg gaacccctgg gcccaggaca aagaccttct tgactatgag gtagacagtg
     2521 acgatgagtg ggaagaggag gaacctgggg agtctttgtc ccacagtgag ggggatgagg
     2581 atgacgatgt gggggaagat gaggatgagg acgatggctt ctttgtgccc catgggtact
     2641 tatctgaaga tgaaggcgtg actgaggagt gtgcagaccc tgagaaccac aaagtacacc
     2701 agaagctgaa ggccaaggag tgggatgagc tcctagccaa gggcaagcgc tttcgtgtgc
     2761 tgcagcctgt gcacgtggga tgtgtatggg catcagaggc agccaactgc acgagttccg
     2821 acttgaagct gctgcagcag ttcactgcat gcctgctgga tgtggcatcc cctgatgagc
     2881 cagaacccgg ggcctccagg agggaaaaga gggaccagca tatcctggcc cagttgcttc
     2941 cgctgctaca tggcaatgtg aatgggagca aggtgatcat ccatgaattc caggagcaat
     3001 gccgcagggg cctgctgacc cttccaagtc ccaccccaca cctgcagatg cccaacttgg
     3061 aggatgctgt tgctgtgcca tccaaggcca ggctgaagcg ccttatctct gagaactctg
     3121 cctatgagaa gcggcccaac ttccgtatgt gctggtatgt gcacccagag gtgctcaaga
     3181 gctttggcca ggagtgcctg ccagtgccct gccagtggac ctacatcacc accatgccct
     3241 cagcacccag ggaagacagt ggcagtgctt ctactgaggg gcctggccag agcacaccta
     3301 tgctactcaa gcggaagcca gcggccacca tgtgcatcac gcagtttatg aagaagcggc
     3361 gctatgatgg acaggttggg tctggggaca tggatggctt ccaggcagac acagaagagg
     3421 atgaagagga tgacacagac tgtatgatca tagatgtgcc agatgttggg agtgacgtgt
     3481 cagaagctcc catacctgct cccacacttt gcaagtgagg atccaccgga ttctagataa
     3541 ctgatcataa
//
