LOCUS       sequence-87053-e        2627 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 604, Emission: 659.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Chu J, Haynes RD, Corbel SY, Li P, Gonzalez-Gonzalez E, Burg JS, 
            Ataie NJ, Lam AJ, Cranfill PJ, Baird MA, Davidson MW, Ng HL, Garcia 
            KC, Contag CH, Shen K, Blau HM, Lin MZ
  TITLE     Non-invasive intravital imaging of cellular differentiation with a 
            bright red-excitable fluorescent protein.
  JOURNAL   Nat Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888.
  PUBMED    24633408
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888."
COMMENT     Sequence Label: mCardinal-Calnexin-N-14
FEATURES             Location/Qualifiers
     source          1..2627
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1852..1861
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1858..2592
                     /codon_start=1
                     /product="far-red fluorescent protein derived from 
                     mNeptune, with further red-shifted spectra"
                     /label=mCardinal
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTTEGEGKPYEGTQTQ
                     RIKVVEGGPLPFAFDILATCFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTVTQDTSLQDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEATTETLYPADGGLEGRCD
                     MALKLVGGGHLHCNLKTTYRSKKPAKNLKMPGVYFVDRRLERIKEADNETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 gatccgctag cgctaccgga ctcagatctc gagaccgcca tggaagggaa gtggttgctg
       61 tgtatgttac tggtgcttgg aactgctatt gttgaggctc atgatggaca tgatgatgat
      121 gtgattgata ttgaggatga ccttgacgat gtcattgaag aggtagaaga ctcaaaacca
      181 gataccactg ctcctccttc atctcccaag gttacttaca aagctccagt tccaacaggg
      241 gaagtatatt ttgctgattc ttttgacaga ggaactctgt cagggtggat tttatccaaa
      301 gccaagaaag acgataccga tgatgaaatt gccaaatatg atggaaagtg ggaggtagag
      361 gaaatgaagg agtcaaagct tccaggtgat aaaggacttg tgttgatgtc tcgggccaag
      421 catcatgcca tctctgctaa actgaacaag cccttcctgt ttgacaccaa gcctctcatt
      481 gttcagtatg aggttaattt ccaaaatgga atagaatgtg gtggtgccta tgtgaaactg
      541 ctttctaaaa caccagaact caacctggat cagttccatg acaagacccc ttatacgatt
      601 atgtttggtc cagataaatg tggagaggac tataaactgc acttcatctt ccgacacaaa
      661 aaccccaaaa cgggtatcta tgaagaaaaa catgctaaga ggccagatgc agatctgaag
      721 acctatttta ctgataagaa aacacatctt tacacactaa tcttgaatcc agataatagt
      781 tttgaaatac tggttgacca atctgtggtg aatagtggaa atctgctcaa tgacatgact
      841 cctcctgtaa atccttcacg tgaaattgag gacccagaag accggaagcc cgaggattgg
      901 gatgaaagac caaaaatccc agatccagaa gctgtcaagc cagatgactg ggatgaagat
      961 gcccctgcta agattccaga tgaagaggcc acaaaacccg aaggctggtt agatgatgag
     1021 cctgagtacg tacctgatcc agacgcagag aaacctgagg attgggatga agacatggat
     1081 ggagaatggg aggctcctca gattgccaac cctagatgtg agtcagctcc tggatgtggt
     1141 gtctggcagc gacctgtgat tgacaacccc aattataaag gcaaatggaa gcctcctatg
     1201 attgacaatc ccagttacca gggaatctgg aaacccagga aaataccaaa tccagatttc
     1261 tttgaagatc tggaaccttt cagaatgact ccttttagtg ctattggttt ggagctgtgg
     1321 tccatgacct ctgacatttt ttttgacaac tttatcattt gtgctgatcg aagaatagtt
     1381 gatgattggg ccaatgatgg atggggcctg aagaaagctg ctgatggggc tgctgagcca
     1441 ggcgttgtgg ggcagatgaa cgaggcagct gaagagcgcc cgtggctgtg ggtagtctat
     1501 attctaactg tagcccttcc tgtgttcctg gttatcctct tctgctgttc tggaaagaaa
     1561 cagaccagtg gtatggagta taagaaaact gatgcacctc aaccggatgt gaaggaagag
     1621 gaagaagaga aggaagagga aaaggacaag ggagatgagg aggaggaagg agaagagaaa
     1681 cttgaagaga aacagaaaag tgatgctgaa gaagatggtg gcactgtcag tcaagaggag
     1741 gaagacagaa aacctaaagc agaggaggat gaaattttga acagatcacc aagaaacaga
     1801 aagccacgaa gagagggggg tgcgtcaggc ggcagcggat ctggaccggt cgccaccatg
     1861 gtgagcaagg gcgaggagct gatcaaggag aacatgcaca tgaagctgta catggaaggc
     1921 accgtgaaca accaccactt caagtgcacc accgaagggg agggcaagcc ctacgagggc
     1981 acccagaccc agaggattaa ggtggtggag ggaggccccc tgccgttcgc attcgacatc
     2041 ctggccacct gctttatgta cgggagcaag accttcatca accacaccca gggcatcccc
     2101 gatttcttta agcagtcctt ccctgagggc ttcacatggg agagagtcac cacatacgaa
     2161 gacgggggcg tgcttaccgt tacccaggac accagcctcc aggacggctg cttgatctac
     2221 aacgtcaagc tcagaggggt gaacttccca tccaacggcc ctgtgatgca gaagaaaaca
     2281 ctcggctggg aggccaccac cgagaccctg taccccgctg acggcggcct ggaaggcaga
     2341 tgcgacatgg ccctgaagct cgtgggcggg ggccacctgc actgcaacct gaagaccaca
     2401 tacagatcca agaaacccgc taagaacctc aagatgcccg gcgtctactt tgtggaccgc
     2461 agactggaaa gaatcaagga ggccgacaat gagacctacg tcgagcagca cgaggtggct
     2521 gtggccagat actgcgacct ccctagcaaa ctggggcaca aacttaatgg catggacgag
     2581 ctgtacaagt aagcggccgc gactctagat cataatcagc catacca
//
