LOCUS       sequence-87055-e        1565 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Gap Junctions, Excitation: 604, Emission: 659.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Chu J, Haynes RD, Corbel SY, Li P, Gonzalez-Gonzalez E, Burg JS, 
            Ataie NJ, Lam AJ, Cranfill PJ, Baird MA, Davidson MW, Ng HL, Garcia 
            KC, Contag CH, Shen K, Blau HM, Lin MZ
  TITLE     Non-invasive intravital imaging of cellular differentiation with a 
            bright red-excitable fluorescent protein.
  JOURNAL   Nat Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888.
  PUBMED    24633408
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888."
COMMENT     Sequence Label: mCardinal-Cx26-7
FEATURES             Location/Qualifiers
     source          1..1565
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      791..800
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             797..1531
                     /codon_start=1
                     /product="far-red fluorescent protein derived from 
                     mNeptune, with further red-shifted spectra"
                     /label=mCardinal
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTTEGEGKPYEGTQTQ
                     RIKVVEGGPLPFAFDILATCFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTVTQDTSLQDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEATTETLYPADGGLEGRCD
                     MALKLVGGGHLHCNLKTTYRSKKPAKNLKMPGVYFVDRRLERIKEADNETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 gatccgctag cgctaccgga ctcagatctc gagctcaagc ttcgaattcg cggctgcacg
       61 ctcctcggga cacagtgcca accatccaga ggacaagatg gattggggca cactacagag
      121 catcctcggg ggtgtcaaca agcactccac cagcattggg aaaatctggc tcactgtcct
      181 cttcatcttc cgcatcatga tcctcgtggt ggccgcgaag gaggtgtggg gagatgagca
      241 agccgatttt gtttgcaaca ctctccagcc tggctgtaag aatgtgtgct acgaccacta
      301 cttccccatc tctcacatcc ggctctgggc tctgcagctg atcatggtgt ccacgccggc
      361 cctcctggta gctatgcacg tggcctaccg gagacacgaa aagaaacgga agttcatgaa
      421 gggagagata aagaacgagt ttaaggacat cgaagagatc aaaacccaga aggtccgtat
      481 cgaagggtcc ctgtggtgga cctacaccac cagcatcttc ttccgggtca tcttcgaagc
      541 tgtcttcatg tatgtctttt acatcatgta caatggcttc ttcatgcagc gtctggtgaa
      601 gtgtaacgcc tggccttgtc ccaatacagt ggactgcttc atttccaggc ccacagaaaa
      661 gactgtcttc acggtgttca tgatctctgt gtctggaatt tgcatcctgc taaacatcac
      721 agagctgtgc tatctgttca ttaggtattg ctcagggaag tccaaaagac cagtcgcgga
      781 tccaccggtc gccaccatgg tgagcaaggg cgaggagctg atcaaggaga acatgcacat
      841 gaagctgtac atggaaggca ccgtgaacaa ccaccacttc aagtgcacca ccgaagggga
      901 gggcaagccc tacgagggca cccagaccca gaggattaag gtggtggagg gaggccccct
      961 gccgttcgca ttcgacatcc tggccacctg ctttatgtac gggagcaaga ccttcatcaa
     1021 ccacacccag ggcatccccg atttctttaa gcagtccttc cctgagggct tcacatggga
     1081 gagagtcacc acatacgaag acgggggcgt gcttaccgtt acccaggaca ccagcctcca
     1141 ggacggctgc ttgatctaca acgtcaagct cagaggggtg aacttcccat ccaacggccc
     1201 tgtgatgcag aagaaaacac tcggctggga ggccaccacc gagaccctgt accccgctga
     1261 cggcggcctg gaaggcagat gcgacatggc cctgaagctc gtgggcgggg gccacctgca
     1321 ctgcaacctg aagaccacat acagatccaa gaaacccgct aagaacctca agatgcccgg
     1381 cgtctacttt gtggaccgca gactggaaag aatcaaggag gccgacaatg agacctacgt
     1441 cgagcagcac gaggtggctg tggccagata ctgcgacctc cctagcaaac tggggcacaa
     1501 acttaatggc atggacgagc tgtacaagta agcggccgcg actctagatc ataatcagcc
     1561 atacc
//
