LOCUS       sequence-87056-e        2172 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Gap Junctions, Excitation: 604, Emission: 659.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Chu J, Haynes RD, Corbel SY, Li P, Gonzalez-Gonzalez E, Burg JS, 
            Ataie NJ, Lam AJ, Cranfill PJ, Baird MA, Davidson MW, Ng HL, Garcia 
            KC, Contag CH, Shen K, Blau HM, Lin MZ
  TITLE     Non-invasive intravital imaging of cellular differentiation with a 
            bright red-excitable fluorescent protein.
  JOURNAL   Nat Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888.
  PUBMED    24633408
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888."
COMMENT     Sequence Label: mCardinal-Cx43-7
FEATURES             Location/Qualifiers
     source          1..2172
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1400..1409
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1406..2140
                     /codon_start=1
                     /product="far-red fluorescent protein derived from 
                     mNeptune, with further red-shifted spectra"
                     /label=mCardinal
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTTEGEGKPYEGTQTQ
                     RIKVVEGGPLPFAFDILATCFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTVTQDTSLQDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEATTETLYPADGGLEGRCD
                     MALKLVGGGHLHCNLKTTYRSKKPAKNLKMPGVYFVDRRLERIKEADNETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 gatccgctag cgctaccgga ctcagatctc gagctcaagc ttcgaattcg gcagcacttt
       61 tctttcattg gggggaaggc gtgaggaaag taccaaacag cagcagactt ttaaacttta
      121 aacagacagg tctgagagcc tgaactctca tttttccttt gacttcagcc tccaaggagt
      181 tccaccaact ttggcgcgcc ggcttcactt tcattaagtg aaagagaggt gcccagacat
      241 gggtgactgg agtgccttgg ggaagcttct ggacaaggtc caagcctact ccaccgctgg
      301 agggaaggtg tggctgtcag tgctcttcat attcagaatc ctgctcctgg ggacagctgt
      361 tgagtcagct tggggtgatg aacagtctgc ctttcgctgt aacactcaac aacctggctg
      421 cgaaaacgtc tgctatgaca agtccttccc catctctcac gtgcgcttct gggtccttca
      481 gatcatattc gtgtctgtgc ccacactcct gtacttggcc catgtgttct atgtgatgag
      541 gaaggaagag aagctaaaca agaaagaaga ggagctcaaa gtggcccaga ctgacggggt
      601 caacgtggag atgcacctga agcagattga aatcaagaag ttcaagtacg ggattgaaga
      661 gcacggcaag gtgaaaatga ggggcggctt gctgagaacc tacatcatca gcatcctctt
      721 caagtctgtc ttcgaggtgg ccttcctgct catccagtgg tacatctatg ggttcagctt
      781 gagcgcggtc tacacctgca agagagatcc ctgcccccac caggtagact gcttcctctc
      841 acgtcccacg gagaaaacca tcttcatcat cttcatgctg gtggtgtcct tggtgtctct
      901 cgctttgaac atcattgagc tcttctacgt cttcttcaaa ggcgttaagg atcgcgtgaa
      961 gggaagaagc gatccttacc acgccaccac tggcccactg agcccatcaa aagactgcgg
     1021 atctccaaaa tacgcctact tcaatggctg ctcctcacca acggctccac tctcgcctat
     1081 gtctcctcct gggtacaagc tggttactgg tgacagaaac aattcctcgt gccgcaatta
     1141 caacaagcaa gctagcgagc aaaactgggc gaactacagc gcagagcaaa atcgcatggg
     1201 gcaggccgga agcaccatct ccaactcgca cgcccagccg ttcgatttcc ccgacgacaa
     1261 ccagaatgcc aaaaaagttg ctgctggaca tgaactccag ccattagcca tcgtggacca
     1321 acgaccttcc agcagagcca gcagccgcgc cagcagcagg cctcggcctg atgacctgga
     1381 gattgcggat ccaccggtcg ccaccatggt gagcaagggc gaggagctga tcaaggagaa
     1441 catgcacatg aagctgtaca tggaaggcac cgtgaacaac caccacttca agtgcaccac
     1501 cgaaggggag ggcaagccct acgagggcac ccagacccag aggattaagg tggtggaggg
     1561 aggccccctg ccgttcgcat tcgacatcct ggccacctgc tttatgtacg ggagcaagac
     1621 cttcatcaac cacacccagg gcatccccga tttctttaag cagtccttcc ctgagggctt
     1681 cacatgggag agagtcacca catacgaaga cgggggcgtg cttaccgtta cccaggacac
     1741 cagcctccag gacggctgct tgatctacaa cgtcaagctc agaggggtga acttcccatc
     1801 caacggccct gtgatgcaga agaaaacact cggctgggag gccaccaccg agaccctgta
     1861 ccccgctgac ggcggcctgg aaggcagatg cgacatggcc ctgaagctcg tgggcggggg
     1921 ccacctgcac tgcaacctga agaccacata cagatccaag aaacccgcta agaacctcaa
     1981 gatgcccggc gtctactttg tggaccgcag actggaaaga atcaaggagg ccgacaatga
     2041 gacctacgtc gagcagcacg aggtggctgt ggccagatac tgcgacctcc ctagcaaact
     2101 ggggcacaaa cttaatggca tggacgagct gtacaagtaa gcggccgcga ctctagatca
     2161 taatcagcca ta
//
