LOCUS       sequence-87059-e        1780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nuclear - Nucleolus, Excitation: 604, Emission: 659.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Chu J, Haynes RD, Corbel SY, Li P, Gonzalez-Gonzalez E, Burg JS, 
            Ataie NJ, Lam AJ, Cranfill PJ, Baird MA, Davidson MW, Ng HL, Garcia 
            KC, Contag CH, Shen K, Blau HM, Lin MZ
  TITLE     Non-invasive intravital imaging of cellular differentiation with a 
            bright red-excitable fluorescent protein.
  JOURNAL   Nat Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888.
  PUBMED    24633408
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888."
COMMENT     Sequence Label: mCardinal-Fibrillarin-7
FEATURES             Location/Qualifiers
     source          1..1780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      23..32
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             29..760
                     /codon_start=1
                     /product="far-red fluorescent protein derived from 
                     mNeptune, with further red-shifted spectra"
                     /label=mCardinal
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTTEGEGKPYEGTQTQ
                     RIKVVEGGPLPFAFDILATCFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTVTQDTSLQDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEATTETLYPADGGLEGRCD
                     MALKLVGGGHLHCNLKTTYRSKKPAKNLKMPGVYFVDRRLERIKEADNETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     CDS             complement(1556..1567)
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing 
                     et al., 2004)"
                     /label=TEE
                     /translation="NHKV"
ORIGIN
        1 agatccgcta gcgctaccgg tcgccaccat ggtgagcaag ggcgaggagc tgatcaagga
       61 gaacatgcac atgaagctgt acatggaagg caccgtgaac aaccaccact tcaagtgcac
      121 caccgaaggg gagggcaagc cctacgaggg cacccagacc cagaggatta aggtggtgga
      181 gggaggcccc ctgccgttcg cattcgacat cctggccacc tgctttatgt acgggagcaa
      241 gaccttcatc aaccacaccc agggcatccc cgatttcttt aagcagtcct tccctgaggg
      301 cttcacatgg gagagagtca ccacatacga agacgggggc gtgcttaccg ttacccagga
      361 caccagcctc caggacggct gcttgatcta caacgtcaag ctcagagggg tgaacttccc
      421 atccaacggc cctgtgatgc agaagaaaac actcggctgg gaggccacca ccgagaccct
      481 gtaccccgct gacggcggcc tggaaggcag atgcgacatg gccctgaagc tcgtgggcgg
      541 gggccacctg cactgcaacc tgaagaccac atacagatcc aagaaacccg ctaagaacct
      601 caagatgccc ggcgtctact ttgtggaccg cagactggaa agaatcaagg aggccgacaa
      661 tgagacctac gtcgagcagc acgaggtggc tgtggccaga tactgcgacc tccctagcaa
      721 actggggcac aaacttaatg gcatggacga gctgtacaag tccggactca gatctcgagc
      781 catgaagcca ggattcagtc cccgtggggg tggctttggc ggccgagggg gctttggtga
      841 ccgtggtggt cgtggaggcc gagggggctt tggcgggggc cgaggccaag gcggaggctt
      901 tagaggtcgt ggacgaggag gaggtggagg cggcggcggc ggtggaggag gaggaagagg
      961 tggtggaggc ttccattttg gtggcaaccg gggtcgtggt cggggaggaa aaagaggaaa
     1021 ccagtcgggg aagaatgtga tggtggagcc gcatcggcat gagggtgtct tcatttgtcg
     1081 gggaaaggaa gatgcactgg tcaccaagaa cctggtccct ggggaatcag tttatggaga
     1141 gaagagagtc tcgatttcgg aaggagatga caaaattgag taccgagcct ggaacccctt
     1201 ccgctccaag cttgcagcag caatcctggg tggtgtggac cagatccaca tcaaaccggg
     1261 ggctaaggtt ctctacctcg gggctgcctc gggcaccacg gtctcccatg tctctgacat
     1321 cgttggtccg gatggtctag tctatgcagt cgagttctcc caccgctctg gccgtgacct
     1381 cattaacttg gccaagaaga ggaccaacat cattcctgtg atcgaggatg ctcgacaccc
     1441 acacaaatac cgcatgctca tcgcaatggt ggatgtgatc tttgctgatg tggcccagcc
     1501 agaccagacc cggattgtgg ccctgaatgc ccacaccttc ctgcgtaatg gaggacactt
     1561 tgtgatttcc attaaggcca actgcattga ctccacagcc tcagccgagg ccgtgtttgc
     1621 ctccgaagtg aaaaagatgc aacaggagaa catgaagccg caggagcagt tgacccttga
     1681 gccatatgaa agagaccatg ccgtggtcgt gggagtgtac aggccacccc ccaaggtgaa
     1741 gaactgagga tccaccggat ctagataact gatcataatc
//
