LOCUS       sequence-87060-e        1400 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 604, Emission: 659.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Chu J, Haynes RD, Corbel SY, Li P, Gonzalez-Gonzalez E, Burg JS, 
            Ataie NJ, Lam AJ, Cranfill PJ, Baird MA, Davidson MW, Ng HL, Garcia 
            KC, Contag CH, Shen K, Blau HM, Lin MZ
  TITLE     Non-invasive intravital imaging of cellular differentiation with a 
            bright red-excitable fluorescent protein.
  JOURNAL   Nat Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888.
  PUBMED    24633408
REFERENCE   2  (bases 1 to 1400)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888."
COMMENT     Sequence Label: mCardinal-H1-10
FEATURES             Location/Qualifiers
     source          1..1400
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      624..633
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             630..1364
                     /codon_start=1
                     /product="far-red fluorescent protein derived from 
                     mNeptune, with further red-shifted spectra"
                     /label=mCardinal
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTTEGEGKPYEGTQTQ
                     RIKVVEGGPLPFAFDILATCFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTVTQDTSLQDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEATTETLYPADGGLEGRCD
                     MALKLVGGGHLHCNLKTTYRSKKPAKNLKMPGVYFVDRRLERIKEADNETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 atccgctagc cgccaccatg accgagaact ccacctccgc cccggcggcg aagcccaaac
       61 gggccaaggc ttccaagaag tccacggacc accccaagta ttcagacatg atcgtggctg
      121 ctatccaggc agagaagaac cgtgccggct cctcgcgcca gtccatccaa aagtatatca
      181 agagccacta caaggtgggt gagaacgccg actcccagat caagttgtcc atcaagcgcc
      241 tagtgaccac cggtgttctc aagcaaacca aaggggtggg cgcctcgggg tccttcaggc
      301 tggccaaggg cgatgagccc aaaaggtcgg tggctttcaa gaagaccaag aaggaagtca
      361 agaaagtggc cactccaaag aaggcagcca agcccaagaa ggctgcctcc aaagccccaa
      421 gcaagaaacc caaagccacc cctgtcaaga aggccaagaa gaagccggct gccacgccca
      481 agaaagccaa aaagcccaag gttgtcaaag tcaaaccagt caaggcctcc aaacccaaga
      541 aggccaaaac cgtgaagccc aaagccaagt cgagtgccaa gagggggagc aagaagaagt
      601 cgggaagcac ggatccaccg gtcgccacca tggtgagcaa gggcgaggag ctgatcaagg
      661 agaacatgca catgaagctg tacatggaag gcaccgtgaa caaccaccac ttcaagtgca
      721 ccaccgaagg ggagggcaag ccctacgagg gcacccagac ccagaggatt aaggtggtgg
      781 agggaggccc cctgccgttc gcattcgaca tcctggccac ctgctttatg tacgggagca
      841 agaccttcat caaccacacc cagggcatcc ccgatttctt taagcagtcc ttccctgagg
      901 gcttcacatg ggagagagtc accacatacg aagacggggg cgtgcttacc gttacccagg
      961 acaccagcct ccaggacggc tgcttgatct acaacgtcaa gctcagaggg gtgaacttcc
     1021 catccaacgg ccctgtgatg cagaagaaaa cactcggctg ggaggccacc accgagaccc
     1081 tgtaccccgc tgacggcggc ctggaaggca gatgcgacat ggccctgaag ctcgtgggcg
     1141 ggggccacct gcactgcaac ctgaagacca catacagatc caagaaaccc gctaagaacc
     1201 tcaagatgcc cggcgtctac tttgtggacc gcagactgga aagaatcaag gaggccgaca
     1261 atgagaccta cgtcgagcag cacgaggtgg ctgtggccag atactgcgac ctccctagca
     1321 aactggggca caaacttaat ggcatggacg agctgtacaa gtaagcggcc gcgactctag
     1381 atcataatca gccataccac
//
